Ectoine biosynthesis genes from the deep sea halophilic eubacteria, Bacillus clausii NIOT-DSB04: Its molecular and biochemical characterization.

Abstract:

:Ectoine, the most prominent osmolyte in nature, is a vital compatible solute present in halophilic bacterium. It protects the cellular biomolecules of the halophilic bacteria and retains their intrinsic function from extreme circumstances. In the current research, ectoine biosynthesis gene cluster (ectABC) in Bacillus clausii NIOT-DSB04 was expressed heterologically in E. coli M15 (pREP4). RP-HPLC resolved several fractions of the purified recombinant product, one of which had been confirmed as ectoine. The recombinant ectoine was further characterized by 1H and 13C Nuclear Magnetic Resonance. The purified recombinant ectoine was also authenticated by FT-IR studies with the existence of ester carbonyl and C-H group. In IPTG induced E. coli M15 transgenic cells, the enzymatic activity of the ectA, B and C genes were found to be higher than that of uninduced cells.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Anburajan L,Meena B,Sreelatha T,Vinithkumar NV,Kirubagaran R,Dharani G

doi

10.1016/j.micpath.2019.103693

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

103693

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(19)30963-5

journal_volume

136

pub_type

杂志文章