当前位置： SCI文献检索 > MICROBIAL PATHOGENESIS期刊下所有文献 > Uptake of Neisserial autotransporter lipoprotein (NalP) promotes an increase in human brain microvascular endothelial cell metabolic activity.

Uptake of Neisserial autotransporter lipoprotein (NalP) promotes an increase in human brain microvascular endothelial cell metabolic activity.

Abstract：

:Neisseria meningitidis is normally a human nasopharyngeal commensal but is also capable of causing life-threatening sepsis and meningitis. N. meningitidis secretes several virulence-associated proteins including Neisserial autotransporter lipoprotein (NalP), an immunogenic, type Va autotransporter harboring an S8-family serine endopeptidase domain. NalP has been previously characterized as a cell-surface maturation protease which processes other virulence-associated meningococcal surface proteins, and as a factor contributing to the survival of meningococci in human serum due to its ability to cleave complement factor C3. Here, recombinant NalP (rNalP) fragments were purified and used to investigate the interaction of NalP with host cells. Flow cytometry and confocal microscopy demonstrated binding and uptake of rNalP into different human cell types. High-resolution microscopy confirmed that internalized rNalP predominantly localized to the perinuclear region of cells. Abolition of rNalP protease activity using site-directed mutagenesis did not influence uptake or sub-cellular localization, but inactive rNalP (rNalPS426A) was unable to induce an increase in human brain microvascular endothelial cell metabolic activity provoked by proteolytically-active rNalP. Our data suggests a more complex and multifaceted role for NalP in meningococcal pathogenesis than was previously understood which includes novel intra-host cell functions.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Dufailu OA,Mahdavi J,Ala'Aldeen DAA,Wooldridge KG,Oldfield NJ

doi

10.1016/j.micpath.2018.08.001

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

70-75

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(18)30476-5

journal_volume

124

pub_type

杂志文章

相关文献

MICROBIAL PATHOGENESIS文献大全
  • Expression and mutagenesis of recombinant cholera toxin A subunit.

    abstract：:ADP-ribosylating protein exotoxins from Vibrio cholerae (CT) and Escherichia coli (LT-I) share two short regions of sequence similarity with Bordetella pertussis toxin (PT). Previous studies have indicated that substitution of arginine for lysine 7 within the first region of CT drastically decreases ADP ribosyltransfe...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1994.1079

    authors： Vadheim KL,Singh Y,Keith JM

    更新日期：1994-11-01 00:00:00

  • Identification of a nuclear targeting signal in YopM from Yersinia spp.

    abstract：:YopM is a type III secretion effector from Yersinia which contributes to pathogenicity but whose action still remains unclear. It is an acidic, leucine-rich repeats (LRR) containing protein which migrates to the nucleus of target cells in spite of the fact that it does not contain any classical nuclear localization si...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2003.12.006

    authors： Benabdillah R,Mota LJ,Lützelschwab S,Demoinet E,Cornelis GR

    更新日期：2004-05-01 00:00:00

  • Immunomodulatory effects and anti-Candida activity of lactobacilli in macrophages and in invertebrate model of Galleria mellonella.

    abstract：:Due to the growing number of multi-resistant Candida spp., adjuvant treatments that may help combat these fungal pathogens are relevant and useful. This study evaluated the immunomodulation and anti-Candida activity of Lactobacillus rhamnosus (LR), Lactobacillus acidophilus and Lactobacillus paracasei suspensions, eit...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.08.006

    authors： de Oliveira FE,Rossoni RD,de Barros PP,Begnini BE,Junqueira JC,Jorge AOC,Leão MVP,de Oliveira LD

    更新日期：2017-09-01 00:00:00

  • Effect of mucin on Campylobacter jejuni association and invasion on HEp-2 cells.

    abstract：:We report the development of an in vitro assay using HEp-2 cell monolayers in which cell association of five C. jejuni strains and E. coli K12 C600 were tested under different experimental conditions. Both total cell associated colony forming units (CFU) and the CFU protected from gentamicin killing (i.e. internalized...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/0882-4010(88)90083-6

    authors： de Melo MA,Pechère JC

    更新日期：1988-07-01 00:00:00

  • An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen.

    abstract：:Rice is an important food crop in the world, and rice blast is one of the major fungal diseases that cause large-scale rice yield reduction. This study aimed to investigate the antagonistic activity and its molecular mechanism of bacterial strain JK isolated from seeds of super hybrid rice 'Shenliangyou 5814' (Oryza s...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104422

    authors： Jing R,Li N,Wang W,Liu Y

    更新日期：2020-10-01 00:00:00

  • Pathophysiology of peste des petits ruminants in sheep (Dorper & Kajli) and goats (Boer & Beetal).

    abstract：:Peste des petits ruminants (PPR), an economically important viral transboundary disease of small ruminants is not only prevalent in Pakistan but also in other countries where people rely on agriculture and animal products. The present study was aimed at describing the pathology and antigen localization in natural PPR ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.02.009

    authors： Khan A,Saleemi MK,Ali F,Abubakar M,Hussain R,Abbas RZ,Khan IA

    更新日期：2018-04-01 00:00:00

  • Alveolar microbiota profile in patients with human pulmonary tuberculosis and interstitial pneumonia.

    abstract：BACKGROUND:The presence of the human lung microbiota has been demonstrated in patients with different lung diseases, mainly in sputum samples. However, for study of the alveolar microbiota, a bronchoalveolar lavage (BAL) sample represents the lower respiratory tract (LRT) environment. It is currently unknown whether th...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103851

    authors： Vázquez-Pérez JA,Carrillo CO,Iñiguez-García MA,Romero-Espinoza I,Márquez-García JE,Falcón LI,Torres M,Herrera MT

    更新日期：2020-02-01 00:00:00

  • The major circulating immunosuppressive activity in American visceral leishmaniasis patients is associated with a high-molecular weight fraction and is not mediated by IgG, IgG immune complexes or lipoproteins.

    abstract：:Opportunistic infections, due to disease-related immunosuppression, constitute the major cause of death in American visceral leishmaniasis (AVL). Sera from these patients (AVL sera) non-specifically inhibit the in vitro proliferative response of normal human lymphocytes to lectins or antigens. In the present work, the...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2006.02.005

    authors： Soares NM,Ferraz TP,Nascimento EG,Carvalho EM,Pontes-de-Carvalho L

    更新日期：2006-06-01 00:00:00

  • Evaluation of antibacterial and antioxidant potential of the zinc oxide nanoparticles synthesized by aqueous and polyol method.

    abstract：:In this paper, we have reported the synthesis, characterization, and evaluation of antimicrobial and antioxidant potential of monodispersed Zinc Oxide (ZnO) nanoparticles synthesized by the room temperature precipitation (aqueous phase) and polyol method (organic phase). ZnO nanoparticle synthesized by both the method...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.03.048

    authors： Soren S,Kumar S,Mishra S,Jena PK,Verma SK,Parhi P

    更新日期：2018-06-01 00:00:00

  • Motility, biofilm formation, apoptotic effect and virulence gene expression of atypical Salmonella Typhimurium outside and inside Caco-2 cells.

    abstract：:Disease outbreaks related to waterborne pathogen contamination throughout the world as well as challenges that lie ahead for addressing persistent infection are of renewed interest. In this research, we studied the effects of prolonged exposure of Salmonella enterica serovar Typhimurium to the cues encountered in the ...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.11.010

    authors： Chakroun I,Mahdhi A,Morcillo P,Cordero H,Cuesta A,Bakhrouf A,Mahdouani K,Esteban MÁ

    更新日期：2018-01-01 00:00:00

  • Resveratrol improves efficacy of oral amoxicillin against childhood fast breathing pneumonia in a randomized placebo-controlled double blind clinical trial.

    abstract：:Childhood pneumonia has been considered as a major cause of child morbidity and mortality worldwide. We aimed to investigate the effect of resveratral in synergizing with oral amoxicillin to improve the treatment outcome of oral amoxicillin administration against childhood fast breathing pneumonia. 647 children diagno...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.micpath.2017.11.062

    authors： Qiang L,Di Y,Jiang Z,Xu J

    更新日期：2018-01-01 00:00:00

  • Identification of viable and non-viable Mycobacterium tuberculosis in mouse organs by directed RT-PCR for antigen 85B mRNA.

    abstract：:Mycobacterium tuberculosis (MTB) the causative organism of tuberculosis can remain dormant as a non-culturable organism, reactivate and cause disease in man and animals. There is a need for proof of viability of such organisms in order to understand the process of reactivation. PCR for bacterial DNA cannot distinguish...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.2000.0353

    authors： Pai SR,Actor JK,Sepulveda E,Hunter RL Jr,Jagannath C

    更新日期：2000-06-01 00:00:00

  • Comparison of the RE-529 sequence and B1 gene for Toxoplasma gondii detection in blood samples of the at-risk seropositive cases using uracil DNA glycosylase supplemented loop-mediated isothermal amplification (UDG-LAMP) assay.

    abstract：:Diagnosis of toxoplasmosis is an important issue, especially in at-risk patients. The molecular methods showed a promising future for such diagnosis; however, the method itself and the target sequence to be detected is an important part of accurate detection of the infection. The aim of the present study was to evalua...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2019.103938

    authors： Valian HK,Mirhendi H,Mohebali M,Shojaee S,Fallahi S,Jafari R,Kheirandish F,Mousavi P

    更新日期：2020-03-01 00:00:00

  • Discrimination between apo and iron-loaded forms of transferrin by transferrin binding protein B and its N-terminal subfragment.

    abstract：:Many pathogens of the Pasteurellaceae and Neisseriaceae possess a surface receptor that binds transferrin (Tf) as an initial step in an iron acquisition process. This receptor is comprised of two proteins, transferrin binding protein A (TbpA) and transferrin binding protein B (TbpB). Since the ability to recognize the...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1998.0226

    authors： Retzer MD,Yu Rh,Zhang Y,Gonzalez GC,Schryvers AB

    更新日期：1998-10-01 00:00:00

  • Comparative analysis of Bacterial Vaginosis microbiota among pregnant and non-pregnant females and isolation of phages against Enterococcus faecalis, Enterococcus faecium, and Shigella flexneri strains.

    abstract：BACKGROUND AND OBJECTIVES:Bacterial vaginosis (BV) is the most common vaginal infection in women of reproductive age. It shifts the paradigms of the vagina from healthy, beneficial microbiota to facultative and strict anaerobes. BV remains one of the most arduous and controversial challenges in modern-day clinical micr...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104588

    authors： Javed A,Manzoor S

    更新日期：2020-12-01 00:00:00

  • hsp65 mRNA+ macrophages and gamma delta T cells in influenza virus-infected mice depleted of the CD4+ and CD8+ lymphocyte subsets.

    abstract：:The effects of depleting CD4+ and CD8+ T cells on macrophage recruitment have been analyzed for bronchoalveolar lavage (BAL) populations from mice with primary or secondary influenza pneumonia. Macrophages were characterized by both the capacity to engulf latex particles and the expression of mRNA for a 65 kD heat sho...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1993.1008

    authors： Allan W,Carding SR,Eichelberger M,Doherty PC

    更新日期：1993-01-01 00:00:00

  • Exosomes carring gag/env of ALV-J possess negative effect on immunocytes.

    abstract：:J subgroup avian leukosis virus (ALV-J) is an exogenous retrovirus of avian. A key feature of ALV-J infection is leading to severe immunosuppressive characteristic of diseases. Viral components of retrovirus were reported closely associated with immunosuppression, and several similarities between exosomes and retrovir...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.09.013

    authors： Wang G,Wang Z,Zhuang P,Zhao X,Cheng Z

    更新日期：2017-11-01 00:00:00

  • Characterization of rickettsial adhesin Adr2 belonging to a new group of adhesins in α-proteobacteria.

    abstract：BACKGROUND:Rickettsia prowazekii is the etiological agent of epidemic typhus and is an obligate intracellular bacterium that grows as a parasite freely within the cytoplasm of a eukaryotic host cell. Previous studies have shown that rOmpA and rOmpB which belong to the family of rickettsial cell surface antigens are inv...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2011.01.009

    authors： Vellaiswamy M,Kowalczewska M,Merhej V,Nappez C,Vincentelli R,Renesto P,Raoult D

    更新日期：2011-05-01 00:00:00

  • Molecular-genetic peculiarities of classical biotype Vibrio cholerae, the etiological agent of the last outbreak Asiatic cholera in Russia.

    abstract：:Molecular-genetic properties of classical biotype Vibrio cholerae strains that caused the Asiatic cholera outbreak in 1942 in Russia have been investigated for the first time. Being characterized by high-level production of cholera toxin and toxin-coregulated adhesion pili both of which are the major virulence factors...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2003.10.004

    authors： Smirnova NI,Cheldyshova NB,Zadnova SP,Kutyrev VV

    更新日期：2004-03-01 00:00:00

  • Epigallocatechin gallate can attenuate human alveolar epithelial cell injury induced by alpha-haemolysin.

    abstract：:Staphylococcus aureus (S. aureus) is a common cause of hospital-acquired infection and has become an epidemic globally. Alpha-haemolysin (α-haemolysin), a pore-forming toxin, is one of the most important virulence factors secreted by most S. aureus strains. α-haemolysin monomers form a 232.4-kDa membrane-inserted hept...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2017.12.054

    authors： Zhou Y,Chen C,Pan J,Deng X,Wang J

    更新日期：2018-02-01 00:00:00

  • Quantitation of H. pylori cytotoxin mRNA by real-time RT-PCR shows a wide expression range that does not correlate with promoter sequences.

    abstract：:Up to 28-fold differences in vacA expression in Helicobacter pylori strains grown in vitro were demonstrated by real time quantitative RT-PCR. These large differences in expression were unrelated to putative -35 and -10 motifs or to other untranslated sequences upstream of the ATG start site. The lack of correlation b...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2004.06.003

    authors： Ayala G,Chihu L,Perales G,Fierros-Zárate G,Hansen LM,Solnick JV,Sánchez J

    更新日期：2004-09-01 00:00:00

  • Chlamydia pneumoniae multiplies in human endothelial cells in vitro.

    abstract：:The ability of three C. pneumoniae isolates, Kajaani 6, Helsinki 12 and TW-183, to grow in human umbilical vein endothelial cells (HUVEC) and in an immortalized endothelial cell line EA.hy 926 was studied. All C. pneumoniae isolates were capable of multiplying in endothelial cells. EA.hy926 cells could support the gro...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1006/mpat.1994.1032

    authors： Kaukoranta-Tolvanen SS,Laitinen K,Saikku P,Leinonen M

    更新日期：1994-04-01 00:00:00

  • Alteration of gene expression in human middle ear epithelial cells induced by influenza A virus and its implication for the pathogenesis of otitis media.

    abstract：:Influenza A virus infection plays a significant role in the pathogenesis of Streptococcus pneumoniae-induced acute otitis media in children. An understanding of how influenza A virus modulates host cellular responses is critically important in efforts to explore the molecular mechanisms of this synergism. We used micr...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2004.06.012

    authors： Tong HH,Long JP,Li D,DeMaria TF

    更新日期：2004-10-01 00:00:00

  • Application of artificial neural networks to describe the combined effect of pH, time, NaCl and ethanol concentrations on the biofilm formation of Staphylococcus aureus.

    abstract：:Biofilms are organized communities, adherent to the surface and resistant to adverse environmental and antimicrobial agents. So, its control is very important. Staphylococcus aureus is an opportunistic pathogen with the biofilm-forming ability that causes numerous problems in the medicine and food industry. Therefore,...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.103986

    authors： Vaezi SS,Poorazizi E,Tahmourespour A,Aminsharei F

    更新日期：2020-04-01 00:00:00

  • Preparation of porcine enterotoxigenic Escherichia coli (ETEC) ghosts and immunogenic analysis in a mouse model.

    abstract：:Enterotoxignenic Escherichia coli (ETEC)-associated colibacillosis causes high levels of morbidity and mortality in neonatal piglets. Vaccination is among effective strategy to fight against ETEC-related diseases. Bacterial ghosts (BGs) are empty bacterial envelopes, which substain subtle antigenic comformation in bac...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2018.11.015

    authors： Ran X,Chen X,Wang S,Chang C,Wen X,Zhai J,Ni H

    更新日期：2019-01-01 00:00:00

  • Detection of genetic diversity in linear plasmids 28-3 and 36 in Borrelia burgdorferi sensu stricto isolates by subtractive hybridization.

    abstract：:Recent studies based on sequence divergence in the ospC gene have identified limited subpopulations of B. burgdorferi associated with invasive human disease. Spirochetes with certain OspC types never cause human disease, while some others cause local infection at the primary skin site but do not hematogenously dissemi...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/s0882-4010(03)00152-9

    authors： Xu Y,Bruno JF,Luft BJ

    更新日期：2003-12-01 00:00:00

  • The cold shock family gene cspD3 is involved in the pathogenicity of Ralstonia solanacearum CQPS-1 to tobacco.

    abstract：:Cold shock proteins (Csps) are small and highly conserved proteins that have target RNA- and DNA-binding activities. Csps play roles in different cellular processes and show functional redundancy. Ralstonia solanacearum, the agent of bacterial wilt, has 4 or 5 Csps based on genome analysis. However, the functions of a...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104091

    authors： Liu Y,Tan X,Cheng H,Gong J,Zhang Y,Wang D,Ding W

    更新日期：2020-02-20 00:00:00

  • Mutation in the S-ribosylhomocysteinase (luxS) gene involved in quorum sensing affects biofilm formation and virulence in a clinical isolate of Aeromonas hydrophila.

    abstract：:A diarrheal isolate SSU of Aeromonas hydrophila produces a cytotoxic enterotoxin (Act) with cytotoxic, enterotoxic, and hemolytic activities. Our laboratory has characterized from the above Aeromonas strain, in addition to Act, the type 3- and T6-secretion systems and their effectors, as well as the genes shown to mod...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2008.08.007

    authors： Kozlova EV,Popov VL,Sha J,Foltz SM,Erova TE,Agar SL,Horneman AJ,Chopra AK

    更新日期：2008-11-01 00:00:00

  • Toxoplasma gondii ROP38 protein: Bioinformatics analysis for vaccine design improvement against toxoplasmosis.

    abstract：:Rhoptry proteins (ROPs) play a significant role in various stages of Toxoplasma gondii (T. gondii) life cycle, being critical for both invasion and intracellular survival. ROP38 is a key manipulator of host gene expression and has a function in tachyzoite to bradyzoite conversion. In this study, we've employed various...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2020.104488

    authors： Nosrati MC,Ghasemi E,Shams M,Shamsinia S,Yousefi A,Nourmohammadi H,Javanmardi E,Kordi B,Majidiani H,Ghaffari AD,Shakarami F

    更新日期：2020-12-01 00:00:00

  • Characterization of non-aflatoxigenic strains of Aspergillus flavus as potential biocontrol agent for the management of aflatoxin contamination in groundnut.

    abstract：:In the present study, nine non-aflatoxigenic Aspergillus flavus strains were evaluated for their potential to reduce A. flavus infection and aflatoxin contamination in groundnut. Genetic characterization of these strains revealed six different deletion patterns (A-F) for thirteen examined genes from the aflatoxin bios...

    journal_title：Microbial pathogenesis

    pub_type： 杂志文章

    doi：10.1016/j.micpath.2016.11.007

    authors： Hulikunte Mallikarjunaiah N,Jayapala N,Puttaswamy H,Siddapura Ramachandrappa N

    更新日期：2017-01-01 00:00:00