The inhibitory effect of the combination of two new peptides on biofilm formation by Acinetobacter baumannii.

Abstract:

:The emergence of extensively drug-resistant (XDR) Acinetobacter baumannii strains and the limited number of efficacious antibiotics demonstrate an urgent need to develop novel agents to treat infections caused by this dangerous pathogen. To find antimicrobial peptides against A. baumannii growing either in planktonic or in biofilm mode, biopanning was carried out with a peptide library on five XDR A. baumannii strains grown in the medium containing human blood (blood biopanning) and biofilms formed by these strains (biofilm biopanning). Two groups of peptides were identified, among which two peptides N10 (from blood biopanning) and NB2 (from biofilm biopanning) were selected and synthesized for more assessments. The selected peptides showed significant binding to A. baumannii rather than to the human cell line Caco-2. Both peptides were effective against A. baumannii and showed antibacterial activities (minimum inhibitory concentration (MIC) 500 μg/ml). In the biofilm inhibition assay, NB2 reduced biofilm more efficiently (75%) than N10 (50%). The combination of the two peptides could function better than each peptide alone to prevent biofilm formation by A. baumannii. Supplementation of conventional therapy with a mixture of peptides targeting A. baumannii or using peptides to deliver antibiotics specifically to the site of infection may be promising to control A. baumannii-related diseases.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Irani N,Basardeh E,Samiee F,Fateh A,Shooraj F,Rahimi A,Shahcheraghi F,Vaziri F,Masoumi M,Pazhouhandeh M,Siadat SD,Kazemi-Lomedasht F,Jamnani FR

doi

10.1016/j.micpath.2018.05.051

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

310-317

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(17)31780-1

journal_volume

121

pub_type

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