The inhibitory impacts of Lactobacillus rhamnosus GG-derived extracellular vesicles on the growth of hepatic cancer cells.

Abstract:

:Bacterial extracellular vesicles (EVs) have come forth into notice as possible important agent to mediate host-pathogen interactions. In this scientific research, the authors have tried to find out the effect of EVs derived from Lactobacillus rhamnosus GG (LDEVs) on the apoptosis induction in HepG2 cell line. The EVs were purified from the conditioned medium of Lactobacillus rhamnosus GG using ultrafiltration and confirmed by transmission electron microscopy (TEM). The HepG2 cells were treated with different concentrations of purified LDEVs and the cytotoxicity and their effects on the expression of bcl-2 and bax genes were assessed by the MTT assay and semi-quantitative RT-PCR, respectively. The MTT assay showed that only 100 μg/ml of LDEVs had a significant cytotoxic effect on cancer cells (p < 0.05). The apoptotic index (bax/bcl2 expression ratio) was significantly increased after treating with 50 and 100 μg/ml LDEVs (p < 0.05). Increased bax/bcl-2 ratio was led to cancer cell death.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Behzadi E,Mahmoodzadeh Hosseini H,Imani Fooladi AA

doi

10.1016/j.micpath.2017.06.016

subject

Has Abstract

pub_date

2017-09-01 00:00:00

pages

1-6

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(17)30474-6

journal_volume

110

pub_type

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