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The role of the temperature-regulated acyltransferase (PA3242) on growth, antibiotic resistance and virulence in Pseudomonas aeruginosa.

Abstract：

:Pseudomonas aeruginosa (PAO1) is an important opportunistic pathogen that thrives in various environments. It is known that the structural variations of the lipopolysaccharide (LPS), including lipid A moiety play an important role in encountering environmental changes. Genes PA3242 and PA0011 have recently been reported to be responsible for secondary-acylation of lipid A in P. aeruginosa. In this study, we confirmed that the PA3242-dependant secondary acylation affects the growth, antibiotic resistance and virulence of PAO1 and functions as a more predominant acyltransferase than PA0011. PA3242 mutant showed inhibited growth at 37 °C and inviability at 28 °C in rich medium LB. The inactivation of PA3242 leads to more sensitivity to a wide range of antibiotics than PAO1(ΔPA0011). Moreover, the virulence of PAO1(ΔPA3242) was attenuated more significantly than that of PAO1 and PAO1(ΔPA0011). The outer membrane integrity and stability of PAO1(ΔPA3242) were seriously compromised. Furthermore, PAO1(ΔPA3242) lost most of pilus and exhibited severely damaged cell envelope, which is probably responsible for the deficiency of swimming, swarming and twitching. These results partially explained the decreased antibiotic resistance and attenuated virulence of PAO1(ΔPA3242) compared to PAO1(ΔPA0011) and PAO1. Our study demonstrated that PA3242-dependent secondary acylation of lipid A plays a predominant role in growth, antibiotic resistance and virulence of PAO1 than PA0011.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Liang Y,Guo Z,Gao L,Guo Q,Wang L,Han Y,Duan K,Shen L

doi

10.1016/j.micpath.2016.09.019

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

126-135

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(16)30315-1

journal_volume

101

pub_type

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