Restriction fragment length polymorphism of PCR amplified papE gene products is correlated with complete serotype among uropathogenic Escherichia coli isolates.

Abstract:

:Using whole bacteria, rather than extracted, purified DNA samples, we amplified the papE gene sequences from 63 Escherichia coli isolates belonging to O serogroups O1, O2 and O6. These isolates were from collections separated temporally as well as geographically: from four cities in the US and one in Sweden. PCR amplified papE products were digested with restriction endonucleases and the relative sizes of the fragments compared for each strain. For 41 of the strains, we found a correlation between the papE restriction fragment length polymorphism (RFLP) and the complete serotype. Furthermore, we were able to detect the presence of duplicate copies of the gene in 14 of the isolates; these 14 isolates were among the 22 that did not exhibit a correlation between the RFLP of their amplified papE sequences and their complete serotype. We conclude that RFLP analysis of PCR products is a rapid and relatively simple method for examining the DNA of E. coli containing the pap gene sequence.

journal_name

Microb Pathog

journal_title

Microbial pathogenesis

authors

Jantausch BA,Hull SI

doi

10.1006/mpat.1996.0033

subject

Has Abstract

pub_date

1996-06-01 00:00:00

pages

351-60

issue

6

eissn

0882-4010

issn

1096-1208

pii

S0882-4010(96)90033-9

journal_volume

20

pub_type

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