Abstract：
:The psychotrophic strain Pseudomonas fluorescens MFO is known to express several enzymatic activities in milk, including extracellular proteolytic activity, optimally when cells are grown at 17.5 degrees C. In order to study the nature of the mechanisms controlling the production of the extracellular protease, we devised a defined medium in which this enzymatic activity was induced by an amino acid and small peptides. Regardless of the inducer, optimal proteolytic activity appeared at 17.5 degrees C. SDS-PAGE and isoelectrofocussing revealed a single protease produced by P. fluorescens MFO with all the inducers used and at all temperatures examined. The level of proteolytic activity correlated with the amount of enzyme in the supernatants.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Hellio FC,Orange N,Guespin-Michel JFdoi
10.1016/0923-2508(93)90064-9subject
Has Abstractpub_date
1993-10-01 00:00:00pages
617-25issue
8eissn
0923-2508issn
1769-7123pii
0923-2508(93)90064-9journal_volume
144pub_type
杂志文章abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.10.009
更新日期：2005-04-01 00:00:00
abstract：:5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90048-7
更新日期：1993-03-01 00:00:00
abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.005
更新日期：2013-01-01 00:00:00
abstract：:OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more se...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01263-3
更新日期：2001-11-01 00:00:00
abstract：:Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.008
更新日期：2016-09-01 00:00:00
abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90003-7
更新日期：1992-02-01 00:00:00
abstract：:Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.05.004
更新日期：2008-07-01 00:00:00
abstract：:The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)01163-3
更新日期：2001-01-01 00:00:00
abstract：:Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.011
更新日期：2016-09-01 00:00:00
abstract：:Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.06.007
更新日期：2019-01-01 00:00:00
abstract：:General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2009.08.018
更新日期：2009-11-01 00:00:00
abstract：:A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00077-9
更新日期：2003-06-01 00:00:00
abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01268-2
更新日期：2001-12-01 00:00:00
abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:In heap bioleaching and waste-rock dumps, complex microbial communities exist in the flowing and interstitial liquid phases and mineral surface-associated biofilms, often embedded in extracellular polymeric substances (EPS). Microbial activity in the interstitial phase and mineral ore surface facilitates mineral degra...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.09.002
更新日期：2020-01-01 00:00:00
abstract：:Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.08.003
更新日期：2014-10-01 00:00:00
abstract：:At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章,评审
doi：10.1016/s0923-2508(00)00116-9
更新日期：2000-03-01 00:00:00
abstract：:Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.06.002
更新日期：2014-09-01 00:00:00
abstract：:Glucose kinase (Glk) from Streptomyces coelicolor was purified, characterized biochemically and kinetically, and compared to Glk from Streptomyces peucetius var. caesius. In both Streptomyces, 96% of the enzyme activity was detected in the cytosolic fraction. The use of a Glk activity gel showed no isoforms of the enz...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.11.001
更新日期：2005-04-01 00:00:00
abstract：:Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Prot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.12.001
更新日期：2012-04-01 00:00:00
abstract：:RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.06.001
更新日期：2020-01-01 00:00:00
abstract：:Since Streptococcus mutans is the principal etiologic agent causing dental caries, by encompassing an array of unique virulence traits, emerging treatment strategies that specifically target the virulence of this pathogen may be promising as alternative approaches compared to conventional antibiotic therapy. In this p...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.004
更新日期：2014-05-01 00:00:00
abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.011
更新日期：2007-04-01 00:00:00
abstract：:Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01313-x
更新日期：2002-05-01 00:00:00
abstract：:In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...
journal_title：Research in microbiology
pub_type： 临床试验,杂志文章,评审
doi：10.1016/0923-2508(90)90114-6
更新日期：1990-09-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)84709-7
更新日期：1996-06-01 00:00:00
abstract：:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00120-5
更新日期：1999-11-01 00:00:00
abstract：:A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.008
更新日期：2014-09-01 00:00:00
