Abstract：
:RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the key activity sites of Escherichia coli RNase E. The results of degradation assays demonstrated that Asp296 is important to RNase E activity in Anabaena sp. PCC 7120 (hereafter PCC 7120). The docking model of RNase E in PCC 7120 (AnaRne) and RNA suggested a possible recognition mechanism of AnaRne to RNA. Moreover, overexpression of AnaRne and its N-terminal catalytic domain (AnaRneN) in vivo led to the abnormal cell division and inhibited the growth of PCC 7120. The quantitative analysis showed a significant decrease of ftsZ transcription in the case of overexpression of AnaRne or AnaRneN and ftsZ mRNA could be directly degraded by AnaRne through degradation assays in vitro, indicating that AnaRne was related to the expression of ftsZ and eventually affected cell division. In essence, our studies expand the understanding of the structural and functional evolutionary basis of RNase E and lay a foundation for further analysis of RNA metabolism in cyanobacteria.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Yan H,Cheng Y,Wang L,Chen Wdoi
10.1016/j.resmic.2020.06.001subject
Has Abstractpub_date
2020-01-01 00:00:00pages
194-202issue
5-6eissn
0923-2508issn
1769-7123pii
S0923-2508(20)30055-3journal_volume
171pub_type
杂志文章abstract：:The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosu...
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doi：10.1016/j.resmic.2009.03.003
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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pub_type： 杂志文章
doi：10.1016/0923-2508(93)90051-3
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.07.008
更新日期：2007-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)81587-2
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pub_type： 杂志文章
doi：10.1016/0923-2508(92)90106-x
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journal_title：Research in microbiology
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更新日期：2018-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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pub_type： 杂志文章
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更新日期：2002-07-01 00:00:00
abstract：:Although this volume is dedicated to honoring François Jacob, I would like my contribution to broaden the context by recalling the background within which the scientists of those times operated. The specific scientific accomplishments of Jacob will certainly be covered by the other contributors who collaborated with h...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
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更新日期：2014-06-01 00:00:00
abstract：:The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.11.003
更新日期：2004-04-01 00:00:00
