Abstract:
:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. Here we report for the first time the presence of an IMP-8 metallo-beta-lactamase gene in the Pseudomonas genus.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Santos C,Caetano T,Ferreira S,Mendo Sdoi
10.1016/j.resmic.2010.03.004subject
Has Abstractpub_date
2010-05-01 00:00:00pages
305-7issue
4eissn
0923-2508issn
1769-7123pii
S0923-2508(10)00057-4journal_volume
161pub_type
杂志文章abstract::Glucose kinase (Glk) from Streptomyces coelicolor was purified, characterized biochemically and kinetically, and compared to Glk from Streptomyces peucetius var. caesius. In both Streptomyces, 96% of the enzyme activity was detected in the cytosolic fraction. The use of a Glk activity gel showed no isoforms of the enz...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.11.001
更新日期:2005-04-01 00:00:00
abstract::Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.06.002
更新日期:2014-09-01 00:00:00
abstract::Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00069-X
更新日期:2003-05-01 00:00:00
abstract::Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.06.007
更新日期:2019-01-01 00:00:00
abstract::By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90020-b
更新日期:1991-02-01 00:00:00
abstract::Macroscopic and microscopic measurements of the growth of Trichoderma viride and Rhizopus oligosporus were compared at different values of water activity of the solid medium. The relationship between radial extension rate and hyphal growth rate was found to be dependent on water activity. The observed difference betwe...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90106-x
更新日期:1992-10-01 00:00:00
abstract::The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2005.12.002
更新日期:2006-05-01 00:00:00
abstract::The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.06.005
更新日期:2016-09-01 00:00:00
abstract::This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, prei...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80042-4
更新日期:1999-01-01 00:00:00
abstract::Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.004
更新日期:2016-10-01 00:00:00
abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90061-r
更新日期:1992-09-01 00:00:00
abstract::While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...
journal_title:Research in microbiology
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.resmic.2014.05.001
更新日期:2014-06-01 00:00:00
abstract::A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.10.009
更新日期:2005-04-01 00:00:00
abstract::The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and properties of the beta-lactamase(s) and the diffusion barrier that the outer-membrane of Gram-negative bacteria can represe...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(91)90084-n
更新日期:1991-07-01 00:00:00
abstract::The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)81383-0
更新日期:1996-05-01 00:00:00
abstract::When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)00110-2
更新日期:1999-09-01 00:00:00
abstract::The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90194-7
更新日期:1993-06-01 00:00:00
abstract::Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.10.004
更新日期:2010-01-01 00:00:00
abstract::Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are increasingly recognized to contribute to lateral gene flow in prokaryotes. ICEs, like most temperate bacteriophages integrate into the genome and like conjugative plasmids disseminate by conjugative transfer to new host...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2004.01.012
更新日期:2004-06-01 00:00:00
abstract::A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2015.01.003
更新日期:2015-02-01 00:00:00
abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01279-7
更新日期:2002-01-01 00:00:00
abstract::Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90128-d
更新日期:1990-09-01 00:00:00
abstract::Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.03.004
更新日期:2004-09-01 00:00:00
abstract::In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01204-9
更新日期:2001-04-01 00:00:00
abstract::Brucella spp. are Gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. As in other Gram-negative bacteria, its S-LPS (smooth lipopolysaccharide) is a major determinant of virulence. The Brucella melitensis 16M LPS O-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. In ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)90130-x
更新日期:2000-10-01 00:00:00
abstract::The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(01)01268-2
更新日期:2001-12-01 00:00:00
abstract::Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.03.003
更新日期:2009-04-01 00:00:00
abstract::This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80078-8
更新日期:1998-03-01 00:00:00
abstract::Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(94)90182-1
更新日期:1994-05-01 00:00:00
abstract::Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2003.11.001
更新日期:2004-03-01 00:00:00