Abstract：
:Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmus maximus), with the Agrobacterium tumefaciens reporter strain KYC55 (pJZ372)(pJZ384)(pJZ410). DA3 produced AHLs during the entire growth phase, and the level of AHLs increased, accompanied by an increase in cell population density. DA3 was also shown to have autoinducer 2 (AI-2) activities. The level of AI-2 activity in DA3 increased rapidly during the exponential phase of growth and maintained a high level up until the stationary phase. The coding sequence for a luxS-like gene of DA3 was obtained showing maximum similarity of 99.6% with that of Vibrio scophthalmi. A mutation in luxS produced no detectable AI-2 activity, but did not result in noticeable changes in growth rate, biofilm formation or virulence of V. ichthyoenteri to turbot, implying that luxS may not play a significant role in regulating virulence gene expression in this bacterium, or that different signal systems need to be inactivated at once in order to decrease virulence. Neither V. ichthyoenteri DA3 nor type strain LMG19664(T) produced any cholerae autoinducer 1 (CAI-1) activity.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Li X,Han Y,Yang Q,Zhang XHdoi
10.1016/j.resmic.2009.10.004subject
Has Abstractpub_date
2010-01-01 00:00:00pages
51-7issue
1eissn
0923-2508issn
1769-7123pii
S0923-2508(09)00197-1journal_volume
161pub_type
杂志文章abstract：:Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.010
更新日期：2016-10-01 00:00:00
abstract：:Quorum sensing is a cell-density-dependent regulatory mechanism which, in Gram-negative bacteria, usually involves the production and detection of N-acyl homoserine lactones (HSLs). In the last four years HSL-dependent quorum sensing has been identified in members of the Burkholderia cepacia complex, and this mini-rev...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2004.01.006
更新日期：2004-05-01 00:00:00
abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01328-1
更新日期：2002-07-01 00:00:00
abstract：:The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2007.09.006
更新日期：2007-12-01 00:00:00
abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.10.009
更新日期：2005-04-01 00:00:00
abstract：:A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.08.004
更新日期：2005-01-01 00:00:00
abstract：:In this review, we synthesise current models and recent comparative genomic studies describing how bacterial species may emerge through adaptation to a new ecological niche and maintain themselves in the same niche over long time periods. We notably consider the impact of genetic exchange with phylogenetically close r...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2015.06.008
更新日期：2015-12-01 00:00:00
abstract：:The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80304-5
更新日期：1998-04-01 00:00:00
abstract：:Monopolization of the vaginal econiche by a limited number of Lactobacillus species, resulting in low pH of 3.5-4.5, has been shown to protect women against vaginal dysbiosis, sexually transmitted infections and adverse pregnancy outcomes. Still, controversy exists as to which characteristics of lactobacilli are most ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.08.001
更新日期：2017-01-01 00:00:00
abstract：:From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.08.002
更新日期：2003-11-01 00:00:00
abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:The structure of spiroplasmas in the Chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. A spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the fi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.04.010
更新日期：2004-10-01 00:00:00
abstract：:Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.09.014
更新日期：2009-12-01 00:00:00
abstract：:Thirty mosquitocidal strains of Bacillus sphaericus isolated from different sources and localities in Brazil were characterized phenotypically and genetically to determine their relationship. Among the strains tested, 93.3% were shown to be resistant to lincomycin, 96.6% to novobiocin, 60% to chloramphenicol and all s...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80032-1
更新日期：1999-03-01 00:00:00
abstract：:It has been suggested that, in rod-shaped bacteria, two sites for peptidoglycan assembly exist: one which is responsible for septum formation and the other, for lateral wall extension. The balance between the activities of these two sites enables bacteria to conserve their own morphology during cell growth. The effect...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)81895-5
更新日期：1997-01-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.10.012
更新日期：2009-03-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:Klebsiella pneumoniae is one of the major Gram-negative bacterial pathogens causing hospital-acquired multidrug-resistant infections, and the antimicrobial treatment options are scarce. The lack of available antimicrobials has prompted the development of alternative strategies for the treatment of these infections. In...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.01.003
更新日期：2019-01-01 00:00:00
abstract：:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01322-0
更新日期：2002-06-01 00:00:00
abstract：:In this study, the use of flow cytometry to analyze phage-mediated killing of Enterobacter aerogenes under varying conditions of temperature and nutrient availability was assessed. Bacteriophage UZ1, specific for an E. aerogenes strain, was applied at a multiplicity of infection (MOI) of 1 and 1000 to a Teflon surface...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.02.007
更新日期：2006-09-01 00:00:00
abstract：:Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.06.002
更新日期：2014-09-01 00:00:00
abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug developm...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/S0923-2508(02)00013-X
更新日期：2003-03-01 00:00:00
abstract：:Development of effective strategies for treatment of candidiasis and other fungal diseases has posed a challenge, considering the increase in opportunistic fungal infections in HIV-positive and immunocompromised patients. The in vitro antifungal activity of essential oil from Ocimum gratissimum was investigated in ord...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.04.004
更新日期：2004-09-01 00:00:00
abstract：:The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.11.003
更新日期：2004-04-01 00:00:00
abstract：:We showed in 1988 that there are two strains of Chlamydia psittaci which infect the koala (Phascolarctos cinereus). In order to further investigate the role of these chlamydial strains in pathogenesis, we have attempted to identify genes of koala type I strain chlamydia which are involved in the immunogenic response. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)83872-7
更新日期：1997-06-01 00:00:00
abstract：:Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)00168-6
更新日期：2000-07-01 00:00:00
abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.03.004
更新日期：2010-05-01 00:00:00
abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.09.005
更新日期：2005-03-01 00:00:00
