Ecological speciation in bacteria: reverse ecology approaches reveal the adaptive part of bacterial cladogenesis.

abstract：:Integrative and conjugative elements (ICEs) are self-transmissible mobile genetic elements that are increasingly recognized to contribute to lateral gene flow in prokaryotes. ICEs, like most temperate bacteriophages integrate into the genome and like conjugative plasmids disseminate by conjugative transfer to new host...

journal_title：Research in microbiology

authors： Burrus V,Waldor MK

更新日期：2004-06-01 00:00:00

abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...

journal_title：Research in microbiology

authors： Cavaglieri L,Orlando J,Rodríguez MI,Chulze S,Etcheverry M

更新日期：2005-06-01 00:00:00

abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...

journal_title：Research in microbiology

authors： Lopatina A,Krylenkov V,Severinov K

更新日期：2013-11-01 00:00:00

abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...

journal_title：Research in microbiology

authors： Kupka D,Liljeqvist M,Nurmi P,Puhakka JA,Tuovinen OH,Dopson M

更新日期：2009-12-01 00:00:00

abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...

journal_title：Research in microbiology

authors： Cloeckaert A,Zygmunt MS,Bézard G,Dubray G

更新日期：1996-05-01 00:00:00

abstract：:Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Prot...

journal_title：Research in microbiology

authors： Talia P,Sede SM,Campos E,Rorig M,Principi D,Tosto D,Hopp HE,Grasso D,Cataldi A

更新日期：2012-04-01 00:00:00

abstract：:An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...

journal_title：Research in microbiology

authors： Matthies C,Gössner A,Acker G,Schramm A,Drake HL

更新日期：2004-12-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...

journal_title：Research in microbiology

authors： Paffetti D,Barberio C,Casalone E,Cavalieri D,Fani R,Fia G,Mori E,Polsinelli M

更新日期：1995-09-01 00:00:00

abstract：:The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...

journal_title：Research in microbiology

authors： Baati H,Guermazi S,Gharsallah N,Sghir A,Ammar E

更新日期：2010-09-01 00:00:00

abstract：:Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...

journal_title：Research in microbiology

authors： Fournier D,Mouton C

更新日期：1993-07-01 00:00:00

abstract：:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis o...

journal_title：Research in microbiology

authors： Han X,Liu L,Fan G,Zhang Y,Xu D,Zuo J,Wang S,Wang X,Tian M,Ding C,Yu S

更新日期：2015-07-01 00:00:00

abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...

journal_title：Research in microbiology

authors： Gulati PS,Virdi JS

更新日期：2007-04-01 00:00:00

abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...

journal_title：Research in microbiology

authors： Hong HA,Khaneja R,Tam NM,Cazzato A,Tan S,Urdaci M,Brisson A,Gasbarrini A,Barnes I,Cutting SM

更新日期：2009-03-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...

journal_title：Research in microbiology

authors： Boumedine KS,Rodolakis A

更新日期：1998-11-01 00:00:00

abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...

journal_title：Research in microbiology

authors： Poly F,Monrozier LJ,Bally R

更新日期：2001-01-01 00:00:00

abstract：:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...

journal_title：Research in microbiology

authors： von Freiesleben U,Rasmussen KV

更新日期：1992-09-01 00:00:00

abstract：:The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at ...

journal_title：Research in microbiology

authors： Castro C,Zhang R,Liu J,Bellenberg S,Neu TR,Donati E,Sand W,Vera M

更新日期：2016-09-01 00:00:00

abstract：:Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...

journal_title：Research in microbiology

authors： Desplats C,Krisch HM

更新日期：2003-05-01 00:00:00

abstract：:The catabolite repressor-activator (Cra) protein controls the direction of carbon flux through metabolic pathways in enteric bacteria. Cra binds to the control regions of target genes and exerts a negative effect on the expression of genes encoding glycolytic and Entner-Doudoroff enzymes, while exerting a positive eff...

journal_title：Research in microbiology

authors： Ramseier TM

更新日期：1996-07-01 00:00:00

abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...

journal_title：Research in microbiology

authors： Bastiaens L,Springael D,Dejonghe W,Wattiau P,Verachtert H,Diels L

更新日期：2001-12-01 00:00:00

abstract：:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...

journal_title：Research in microbiology

authors： Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai K

更新日期：2015-11-01 00:00:00

abstract：:The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...

journal_title：Research in microbiology

authors： Ribeiro SM,Cavalcanti LH,Pereira EC,Silva NH

更新日期：2003-01-01 00:00:00

abstract：:KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in...

journal_title：Research in microbiology

authors： Mazzoni C,Santori F,Saliola M,Falcone C

更新日期：2000-01-01 00:00:00

abstract：:The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...

journal_title：Research in microbiology

authors： Vasilieva S

更新日期：2002-09-01 00:00:00

abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...

journal_title：Research in microbiology

authors： Masi M,Dumont E,Vergalli J,Pajovic J,Réfrégiers M,Pagès JM

更新日期：2018-01-01 00:00:00

abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...

journal_title：Research in microbiology

authors： Ercolini D,Fusco V,Blaiotta G,Coppola S

更新日期：2005-03-01 00:00:00

abstract：:Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...

journal_title：Research in microbiology

authors： Serra R,Braga A,Venâncio A

更新日期：2005-05-01 00:00:00

abstract：:A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...

journal_title：Research in microbiology

authors： Li X,He J,Li S

更新日期：2007-03-01 00:00:00

abstract：:The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...

journal_title：Research in microbiology

authors： Edskes HK

更新日期：2001-09-01 00:00:00

abstract：:The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...

journal_title：Research in microbiology

authors： Ivannikova YV,Naumova ES,Naumov GI

更新日期：2007-10-01 00:00:00