Abstract：
:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses, were the most discriminating for the study of nifH gene diversity as they resulted in differences between strains at the species level. Amplification by selected primers and RFLP were applied to assess the genetic diversity of the nifH gene pool in soil. Pair soils, one under cultivation, the second under permanent pasture, were found to harbor a contrasting diversity of nifH genes. Pure strain profiles could not be recognized in the nifH soil patterns. Using the simple procedure described, it was shown that the structure of nitrogen fixers in soil was influenced by soil functioning.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Poly F,Monrozier LJ,Bally Rdoi
10.1016/s0923-2508(00)01172-4subject
Has Abstractpub_date
2001-01-01 00:00:00pages
95-103issue
1eissn
0923-2508issn
1769-7123pii
S0923250800011724journal_volume
152pub_type
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journal_title：Research in microbiology
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doi：10.1016/0923-2508(90)90019-m
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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更新日期：2005-04-01 00:00:00
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journal_title：Research in microbiology
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doi：10.1016/s0923-2508(99)80077-1
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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abstract：:rRNA gene restriction patterns (ribotyping) were compared with phage typing, serotyping, enterotoxins and exfoliatin production in the analysis of 26 Staphylococcus aureus strains isolated from two different nosocomial outbreaks. Total DNA was cleaved by EcoRI restriction endonuclease. After agarose gel electrophoresi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90212-k
更新日期：1993-01-01 00:00:00
abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
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