Abstract:
:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of sporulation mutants of Bacillus subtilis. One of these genes, Kin1377, significantly restored the sporulation deficiency of kinA kinB double mutant of B. subtilis, but not of B. subtilis spo0B mutant. These results indicated that Kin1377 requires B. subtilis Spo0B and possibly Spo0F to transfer phosphate to B. subtilis Spo0A. Another putative kinase, Kin1038, slightly restored the sporulation deficiencies of both kinA kinB double mutant and spo0B mutant of B. subtilis. However the sporulation deficiency of the B. subtilis spo0B mutant was significantly restored in the presence of both Kin1038 and P. polymyxa Spo0A. These results indicate that the overexpressed Kin1038 is able to interact directly with and activate P. polymyxa Spo0A, and that Spo0A can support spore formation in B. subtilis.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Park SY,Park SH,Choi SKdoi
10.1016/j.resmic.2012.02.003subject
Has Abstractpub_date
2012-05-01 00:00:00pages
272-8issue
4eissn
0923-2508issn
1769-7123pii
S0923-2508(12)00028-9journal_volume
163pub_type
杂志文章abstract::Thirty mosquitocidal strains of Bacillus sphaericus isolated from different sources and localities in Brazil were characterized phenotypically and genetically to determine their relationship. Among the strains tested, 93.3% were shown to be resistant to lincomycin, 96.6% to novobiocin, 60% to chloramphenicol and all s...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80032-1
更新日期:1999-03-01 00:00:00
abstract::The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2007.09.006
更新日期:2007-12-01 00:00:00
abstract::Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)84709-7
更新日期:1996-06-01 00:00:00
abstract::5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90048-7
更新日期:1993-03-01 00:00:00
abstract::We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.03.002
更新日期:2007-06-01 00:00:00
abstract::In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90016-j
更新日期:1990-06-01 00:00:00
abstract::Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.03.006
更新日期:2018-01-01 00:00:00
abstract::The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.07.006
更新日期:2006-04-01 00:00:00
abstract::Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01373-6
更新日期:2002-11-01 00:00:00
abstract::We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains Al12(T) and Al16 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg o...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.06.004
更新日期:2006-11-01 00:00:00
abstract::Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2003.11.001
更新日期:2004-03-01 00:00:00
abstract::The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90060-2
更新日期:1992-09-01 00:00:00
abstract::The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90194-7
更新日期:1993-06-01 00:00:00
abstract::Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.009
更新日期:2016-10-01 00:00:00
abstract::Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)00145-5
更新日期:2000-05-01 00:00:00
abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90061-r
更新日期:1992-09-01 00:00:00
abstract::Although this volume is dedicated to honoring François Jacob, I would like my contribution to broaden the context by recalling the background within which the scientists of those times operated. The specific scientific accomplishments of Jacob will certainly be covered by the other contributors who collaborated with h...
journal_title:Research in microbiology
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.resmic.2014.05.027
更新日期:2014-06-01 00:00:00
abstract::The association between bacteria and leaves in Ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. This intimate interaction suggests that both partners have co-diversified to...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.04.003
更新日期:2011-06-01 00:00:00
abstract::Ecologists have documented the process of plant succession for centuries, yet the successional patterns exhibited by microbial communities have received relatively little attention. We examine recent work on microbial succession and show how, despite some key differences, studies of plant succession can serve as a tem...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2010.06.002
更新日期:2010-10-01 00:00:00
abstract::Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00148-7
更新日期:2003-10-01 00:00:00
abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.11.020
更新日期:2008-03-01 00:00:00
abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90191-c
更新日期:1991-06-01 00:00:00
abstract::The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or externa...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90024-7
更新日期:1989-05-01 00:00:00
abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80037-0
更新日期:1999-04-01 00:00:00
abstract::The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)00008-6
更新日期:2003-01-01 00:00:00
abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title:Research in microbiology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.resmic.2014.09.007
更新日期:2015-05-01 00:00:00
abstract::Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01330-x
更新日期:2002-10-01 00:00:00
abstract::Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.03.001
更新日期:2014-04-01 00:00:00
abstract::The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(01)01268-2
更新日期:2001-12-01 00:00:00