Abstract：
:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surface ferric reductase activity in Candida albicans, revealing that there existed functional redundancy and/or a compensatory upregulation mechanism among ferric reductase genes. The absence of CFL1 resulted in increased expression levels of other alternative ferric reductase genes, including FRP1, CFL2 and FRE10. In addition, CFL1 played an important role in the response to different oxidative stresses. Further research revealed that the cfl1Δ/Δ mutant exhibited higher levels of both ROS production and SOD activity under oxidative conditions. Moreover, deletion of CFL1 led to a profound defect in filamentous development in an iron-independent manner at both 30 and 37 °C. The cfl1Δ/Δ mutant exhibited highly attenuated virulence and reduced fungal burdens in the mouse systemic infection model, indicating that CFL1 might be a potential target for antifungal drug development. In summary, our results provide new insights into the roles of ferric reductase gene in C. albicans.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Xu N,Qian K,Dong Y,Chen Y,Yu Q,Zhang B,Xing L,Li Mdoi
10.1016/j.resmic.2014.03.001subject
Has Abstractpub_date
2014-04-01 00:00:00pages
252-61issue
3eissn
0923-2508issn
1769-7123pii
S0923-2508(14)00029-1journal_volume
165pub_type
杂志文章abstract：:Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.07.001
更新日期：2012-07-01 00:00:00
abstract：:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.10.007
更新日期：2012-01-01 00:00:00
abstract：:Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90075-y
更新日期：1992-11-01 00:00:00
abstract：:Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.09.003
更新日期：2007-10-01 00:00:00
abstract：:For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title：Research in microbiology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.resmic.2014.09.007
更新日期：2015-05-01 00:00:00
abstract：:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis o...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.06.004
更新日期：2015-07-01 00:00:00
abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90059-y
更新日期：1990-07-01 00:00:00
abstract：:The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00130-8
更新日期：1999-11-01 00:00:00
abstract：:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00120-5
更新日期：1999-11-01 00:00:00
abstract：:When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)00110-2
更新日期：1999-09-01 00:00:00
abstract：:The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and properties of the beta-lactamase(s) and the diffusion barrier that the outer-membrane of Gram-negative bacteria can represe...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90084-n
更新日期：1991-07-01 00:00:00
abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.11.020
更新日期：2008-03-01 00:00:00
abstract：:By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90020-b
更新日期：1991-02-01 00:00:00
abstract：:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80003-x
更新日期：1998-07-01 00:00:00
abstract：:During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90086-7
更新日期：1989-10-01 00:00:00
abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.01.001
更新日期：2010-03-01 00:00:00
abstract：:Scedosporium apiospermum, a recently isolated phenol-degrading hyphomycete, was shown to be able to productively utilise the diaryl ester phenylbenzoate as its sole source of carbon and energy. The characterisation of degradation intermediates together with the detection of the corresponding catabolic enzymes in crude...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80077-1
更新日期：1999-07-01 00:00:00
abstract：:A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 d...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90062-0
更新日期：1994-01-01 00:00:00
abstract：:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90078-7
更新日期：1994-10-01 00:00:00
abstract：:Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive wit...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.02.005
更新日期：2015-04-01 00:00:00
abstract：:Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90026-k
更新日期：1992-03-01 00:00:00
abstract：:The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01239-6
更新日期：2001-09-01 00:00:00
abstract：:In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01204-9
更新日期：2001-04-01 00:00:00
abstract：:KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)00131-5
更新日期：2000-01-01 00:00:00
abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80565-1
更新日期：1995-09-01 00:00:00
abstract：:Benzene is biodegraded in the absence of oxygen under a variety of terminal electron-accepting conditions. However, the mechanism by which anaerobic benzene degradation occurs is unclear. Phenol and benzoate have been consistently detected as intermediates of anaerobic benzene degradation, suggesting that the hydroxyl...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01378-5
更新日期：2002-12-01 00:00:00
abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00024-X
更新日期：2003-04-01 00:00:00
abstract：:The outer membrane of gram-negative bacteria acts as a barrier against harmful lipophilic compounds and larger molecules unable to diffuse freely through the porins. However, outer membrane proteins together with the Tol-Pal and TonB systems have been exploited for the entry of macromolecules such as bacteriocins and ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01226-8
更新日期：2001-07-01 00:00:00
abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01172-4
更新日期：2001-01-01 00:00:00
abstract：:The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(97)83624-8
更新日期：1998-01-01 00:00:00
