Novel role of the Candida albicans ferric reductase gene CFL1 in iron acquisition, oxidative stress tolerance, morphogenesis and virulence.


:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surface ferric reductase activity in Candida albicans, revealing that there existed functional redundancy and/or a compensatory upregulation mechanism among ferric reductase genes. The absence of CFL1 resulted in increased expression levels of other alternative ferric reductase genes, including FRP1, CFL2 and FRE10. In addition, CFL1 played an important role in the response to different oxidative stresses. Further research revealed that the cfl1Δ/Δ mutant exhibited higher levels of both ROS production and SOD activity under oxidative conditions. Moreover, deletion of CFL1 led to a profound defect in filamentous development in an iron-independent manner at both 30 and 37 °C. The cfl1Δ/Δ mutant exhibited highly attenuated virulence and reduced fungal burdens in the mouse systemic infection model, indicating that CFL1 might be a potential target for antifungal drug development. In summary, our results provide new insights into the roles of ferric reductase gene in C. albicans.


Res Microbiol


Research in microbiology


Xu N,Qian K,Dong Y,Chen Y,Yu Q,Zhang B,Xing L,Li M




Has Abstract


2014-04-01 00:00:00














  • Isolation, purification and partial analysis of the lipopolysaccharide antigenic determinant recognized by a monoclonal antibody to Legionella pneumophila serogroup 1.

    abstract::Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Petitjean F,Dournon E,Strosberg AD,Hoebeke J

    更新日期:1990-11-01 00:00:00

  • The Institut Pasteur attic dwellers: their origins, their paths to discovery.

    abstract::Although this volume is dedicated to honoring François Jacob, I would like my contribution to broaden the context by recalling the background within which the scientists of those times operated. The specific scientific accomplishments of Jacob will certainly be covered by the other contributors who collaborated with h...

    journal_title:Research in microbiology

    pub_type: 传,历史文章,杂志文章


    authors: Cohn M

    更新日期:2014-06-01 00:00:00

  • Mitochondrial ABC transporters.

    abstract::In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


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  • Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes.

    abstract::This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Sibold L,Henriquet M,Possot O,Aubert JP

    更新日期:1991-01-01 00:00:00

  • Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.

    abstract::The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Koblavi S,Grimont F,Grimont PA

    更新日期:1990-07-01 00:00:00

  • Limited selection of sodium channel blocking toxin-producing bacteria from paralytic shellfish toxin-contaminated mussels (Aulacomya ater).

    abstract::Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Vásquez M,Grüttner C,Möeller B,Moore ER

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  • Results of passive and active immunization directed against ferric aerobactin in experimental enterobacterial infections in mice and chickens.

    abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Le Roy D,Crouzier C,Dho-Moulin M,Dumont AS,Bouchet A,Lafont JP,Andremont A

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  • Phylogenetic affiliation of Pseudomonas putida biovar A and B strains.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


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  • Lactovum miscens gen. nov., sp. nov., an aerotolerant, psychrotolerant, mixed-fermentative anaerobe from acidic forest soil.

    abstract::An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Matthies C,Gössner A,Acker G,Schramm A,Drake HL

    更新日期:2004-12-01 00:00:00

  • Regulation of expression of the tricarballylate utilization operon (tcuABC) of Salmonella enterica.

    abstract::The tricarballylate utilization locus (tcuRABC) of Salmonella enterica serovar Typhimurium is comprised of a 3-gene operon (tcuABC) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcuR gene, which encodes a putative LysR-type transcriptional regulator. ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Lewis JA,Stamper LW,Escalante-Semerena JC

    更新日期:2009-04-01 00:00:00

  • A transcriptional luxAB reporter fusion responding to fluorene in Sphingomonas sp. LB126 and its initial characterisation for whole-cell bioreporter purposes.

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  • A century of typhus, lice and Rickettsia.

    abstract::At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...

    journal_title:Research in microbiology

    pub_type: 传,历史文章,杂志文章,评审


    authors: Andersson JO,Andersson SG

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  • Deciphering the molecular basis of multidrug recognition: crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR.

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    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


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  • Characterization and chromosomal organization of the murD-murC-ftsQ region of Corynebacterium glutamicum ATCC 13869.

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    journal_title:Research in microbiology

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  • A combination of a SEM technique and X-ray microanalysis for studying the spore germination process of Clostridium tyrobutyricum.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Bassi D,Cappa F,Cocconcelli PS

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  • Rhodobacter massiliensis sp. nov., a new amoebae-resistant species isolated from the nose of a patient.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


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  • Multiple fungal enzymes possess cysteine synthase activity in vitro.

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    journal_title:Research in microbiology

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    journal_title:Research in microbiology

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    journal_title:Research in microbiology

    pub_type: 杂志文章


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    journal_title:Research in microbiology

    pub_type: 杂志文章


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  • Extraction, partial purification and characterization of a bacteriocin (fragicilin) produced by a strain of Bacteroides fragilis isolated from Callithrix penicillata.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


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    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


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  • Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Lescure T,Joulian C,Charles C,Ben Ali Saanda T,Charron M,Breeze D,Bauda P,Battaglia-Brunet F

    更新日期:2020-01-01 00:00:00

  • Characterization of sporulation histidine kinases of Paenibacillus polymyxa.

    abstract::Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Park SY,Park SH,Choi SK

    更新日期:2012-05-01 00:00:00

  • How François Jacob bridged the gap between the "two cultures".

    abstract::While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...

    journal_title:Research in microbiology

    pub_type: 传,历史文章,杂志文章


    authors: Morange M

    更新日期:2014-06-01 00:00:00

  • Induction of lactate production associated with a decrease in NADH cell content enables growth resumption of Clostridium cellulolyticum in batch cultures on cellobiose.

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    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Payot S,Guedon E,Gelhaye E,Petitdemange H

    更新日期:1999-09-01 00:00:00

  • Acidithrix ferrooxidans gen. nov., sp. nov.; a filamentous and obligately heterotrophic, acidophilic member of the Actinobacteria that catalyzes dissimilatory oxido-reduction of iron.

    abstract::A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Jones RM,Johnson DB

    更新日期:2015-02-01 00:00:00

  • rRNA gene restriction patterns of Legionella species: a molecular identification system.

    abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...

    journal_title:Research in microbiology

    pub_type: 杂志文章


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    更新日期:1989-11-01 00:00:00

  • Anaerobic transformation of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) by ovine rumen microorganisms.

    abstract::Explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) provide a challenge in terms of bioremediation. In the present study, sheep rumen was studied for its potential to detoxify HMX using analytical chemistry and molecular microbial ecology tools. Results indicated significant loss (p < 0.05) of HM...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Perumbakkam S,Craig AM

    更新日期:2012-09-01 00:00:00