Hybridization of transposon Tn501 for detection of mercury resistance sequences in a marine environment.

abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...

journal_title：Research in microbiology

authors： Mocali S,Benedetti A

更新日期：2010-07-01 00:00:00

abstract：:The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...

journal_title：Research in microbiology

authors： Youenou B,Brothier E,Nazaret S

更新日期：2014-01-01 00:00:00

abstract：:The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...

journal_title：Research in microbiology

authors： Willems A,East AK,Lawson PA,Collins MD

更新日期：1993-09-01 00:00:00

abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...

journal_title：Research in microbiology

authors： Ledgham F,Soscia C,Chakrabarty A,Lazdunski A,Foglino M

更新日期：2003-04-01 00:00:00

abstract：:The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...

journal_title：Research in microbiology

authors： Smith HO,Gwinn ML,Salzberg SL

更新日期：1999-11-01 00:00:00

abstract：:Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...

journal_title：Research in microbiology

authors： Mangin I,Bourget N,Simonet JM,Decaris B

更新日期：1995-01-01 00:00:00

abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...

journal_title：Research in microbiology

authors： Colón-González M,Méndez-Ortiz MM,Membrillo-Hernández J

更新日期：2004-09-01 00:00:00

abstract：:Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...

journal_title：Research in microbiology

authors： Lemaire ON,Honoré FA,Jourlin-Castelli C,Méjean V,Fons M,Iobbi-Nivol C

更新日期：2016-10-01 00:00:00

abstract：:The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...

journal_title：Research in microbiology

authors： Gailus V,Ramsperger U,Johner C,Kramer H,Rasched I

更新日期：1994-11-01 00:00:00

abstract：:A variety of pathways for carbon and electron flow in the bacterium Escherichia coli and in other enteric bacteria are differentially expressed depending on whether molecular oxygen is present in the cell environment. This review briefly summarizes the metabolic pathways operative during aerobic versus anaerobic cell ...

journal_title：Research in microbiology

authors： Gunsalus RP,Park SJ

更新日期：1994-06-01 00:00:00

abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...

journal_title：Research in microbiology

authors： Pérez-Montaño F,Guasch-Vidal B,González-Barroso S,López-Baena FJ,Cubo T,Ollero FJ,Gil-Serrano AM,Rodríguez-Carvajal MÁ,Bellogín RA,Espuny MR

更新日期：2011-09-01 00:00:00

abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...

journal_title：Research in microbiology

authors： Voulhoux R,Tommassen J

更新日期：2004-04-01 00:00:00

abstract：:The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...

journal_title：Research in microbiology

authors： Ramos A,Honrubia MP,Vega D,Ayala JA,Bouhss A,Mengin-Lecreulx D,Gil JA

更新日期：2004-04-01 00:00:00

abstract：:Measuring gene expression at the single cell and single molecule level has recently made possible the quantitative measurement of stochasticity of gene expression. This enables identification of the probable sources and roles of noise. Gene expression noise can result in bacterial population heterogeneity, offering sp...

journal_title：Research in microbiology

authors： Bury-Moné S,Sclavi B

更新日期：2017-01-01 00:00:00

abstract：:The response of Acidithiobacillus ferrooxidans ATCC 23270 to copper was analyzed in sulfur-grown cells by using quantitative proteomics. Forty-seven proteins showed altered levels in cells grown in the presence of 50 mM copper sulfate. Of these proteins, 24 were up-regulated and 23 down-regulated. As seen before in fe...

journal_title：Research in microbiology

authors： Almárcegui RJ,Navarro CA,Paradela A,Albar JP,von Bernath D,Jerez CA

更新日期：2014-11-01 00:00:00

abstract：:The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...

journal_title：Research in microbiology

authors： Fenice M,Selbmann L,Di Giambattista R,Federici F

更新日期：1998-04-01 00:00:00

abstract：:A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...

journal_title：Research in microbiology

authors： Tsang RS,Schlecht S,Aleksic S,Chan KH,Chau PY

更新日期：1991-06-01 00:00:00

abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...

journal_title：Research in microbiology

authors： Kupka D,Liljeqvist M,Nurmi P,Puhakka JA,Tuovinen OH,Dopson M

更新日期：2009-12-01 00:00:00

abstract：:Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...

journal_title：Research in microbiology

authors： Bernhardt TG,Wang IN,Struck DK,Young R

更新日期：2002-10-01 00:00:00

abstract：:Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...

journal_title：Research in microbiology

authors： Petitjean F,Dournon E,Strosberg AD,Hoebeke J

更新日期：1990-11-01 00:00:00

abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...

journal_title：Research in microbiology

authors： Rotem O,Biran D,Ron EZ

更新日期：2013-01-01 00:00:00

abstract：:A collection of 57 strains of hyperthermophilic Archaea from the order Thermococcales was screened for the presence of plasmids; 9 plasmids present in six of these strains were isolated and characterized in terms of size and cross-hybridization. The Notl macrorestriction patterns of genomic DNA of strains harbouring t...

journal_title：Research in microbiology

authors： Benbouzid-Rollet N,López-García P,Watrin L,Erauso G,Prieur D,Forterre P

更新日期：1997-12-01 00:00:00

abstract：:Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...

journal_title：Research in microbiology

authors： Llanes C,Couturier M,Asfeld L,Grimont F,Michel-Briand Y

更新日期：1994-01-01 00:00:00

abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...

journal_title：Research in microbiology

authors： Lopatina A,Krylenkov V,Severinov K

更新日期：2013-11-01 00:00:00

abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...

journal_title：Research in microbiology

authors： Santos C,Caetano T,Ferreira S,Mendo S

更新日期：2010-05-01 00:00:00

abstract：:We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...

journal_title：Research in microbiology

authors： Brzywczy J,Natorff R,Sieńko M,Paszewski A

更新日期：2007-06-01 00:00:00

abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...

journal_title：Research in microbiology

authors： Matthijs S,Koedam N,Cornelis P,De Greve H

更新日期：2000-12-01 00:00:00

abstract：:This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

journal_title：Research in microbiology

authors： Sibold L,Henriquet M,Possot O,Aubert JP

更新日期：1991-01-01 00:00:00

abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

journal_title：Research in microbiology

authors： Chai BF,Wang W,Liang AH

更新日期：2006-04-01 00:00:00

abstract：:A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...

journal_title：Research in microbiology

authors： Garcerá-Teruel A,Xoconostle-Cázares B,Rosas-Quijano R,Ortiz L,León-Ramírez C,Specht CA,Sentandreu R,Ruiz-Herrera J

更新日期：2004-03-01 00:00:00