Abstract：
:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of biofilm after 4 to 8 h, displaying maximal accumulation of formed biofilm at 24 h, corresponding to the onset of stationary phase. Then an abrupt reduction in the biomass of the biofilm was observed. This decrease was not prevented by external addition of fresh nutrients and coincided with the depletion of oxygen as measured by the enzymatic activity of the AdhE protein. No biofilm formation was detected in cultures grown anaerobically in LB or LB supplemented with nitrate, nitrite, DMSO or fumarate, even after 72 h of incubation, well inside the stationary phase, suggesting that under anaerobic growth conditions E. coli cannot form biofilms.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Colón-González M,Méndez-Ortiz MM,Membrillo-Hernández Jdoi
10.1016/j.resmic.2004.03.004subject
Has Abstractpub_date
2004-09-01 00:00:00pages
514-21issue
7eissn
0923-2508issn
1769-7123pii
S0923-2508(04)00076-2journal_volume
155pub_type
杂志文章abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...
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abstract：:While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
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abstract：:This paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of L. monocyto...
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.06.004
更新日期：2015-07-01 00:00:00
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abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
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pub_type： 杂志文章
doi：10.1016/j.resmic.2005.08.001
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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doi：10.1016/j.resmic.2008.05.004
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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