Abstract：
:Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing abortion in ruminants and 2 strains from faecal samples. For the AFLP analysis, the total purified genomic DNA of each strain was submitted to a one-step digestion-ligation reaction for 3 h at 37 degrees C. DNA was digested with a single restriction endonuclease Mspl and ligated to specially constructed adapters. Subsequently, restricted fragments were selectively amplified under high stringency PCR conditions using primers complementary to the adapters. Amplified products were then resolved on agarose gel electrophoresis. The method is easy to perform, fast and reproducible. AFLP enabled characterization of C. psittaci strains at the infra-subspecific level. Thus, AFLP led to the identification of a cluster of strains on the basis of their AFLP patterns, constituted by French chlamydial isolates. It also permitted differentiation among strains in relation to host origin and to clinical syndromes. These data confirmed the highly discriminative power of AFLP towards the differentiation of closely related ruminant C. psittaci strains. The analysis will need to be applied to more samples to check the usefulness of AFLP markers in epidemiological and evolutionary studies.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Boumedine KS,Rodolakis Adoi
10.1016/s0923-2508(99)80020-5subject
Has Abstractpub_date
1998-11-01 00:00:00pages
735-44issue
10eissn
0923-2508issn
1769-7123pii
S0923250899800205journal_volume
149pub_type
杂志文章abstract：:OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more se...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01263-3
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abstract：:The catabolite repressor-activator (Cra) protein controls the direction of carbon flux through metabolic pathways in enteric bacteria. Cra binds to the control regions of target genes and exerts a negative effect on the expression of genes encoding glycolytic and Entner-Doudoroff enzymes, while exerting a positive eff...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(96)84003-4
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abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
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更新日期：2018-04-01 00:00:00
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journal_title：Research in microbiology
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doi：10.1016/s0923-2508(01)01268-2
更新日期：2001-12-01 00:00:00
abstract：:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis o...
journal_title：Research in microbiology
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doi：10.1016/j.resmic.2015.06.004
更新日期：2015-07-01 00:00:00
abstract：:Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01373-6
更新日期：2002-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80078-8
更新日期：1998-03-01 00:00:00
abstract：:A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90185-d
更新日期：1991-06-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90117-7
更新日期：1992-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.08.022
更新日期：2009-12-01 00:00:00
abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.07.001
更新日期：2011-10-01 00:00:00
abstract：:Glucose kinase (Glk) from Streptomyces coelicolor was purified, characterized biochemically and kinetically, and compared to Glk from Streptomyces peucetius var. caesius. In both Streptomyces, 96% of the enzyme activity was detected in the cytosolic fraction. The use of a Glk activity gel showed no isoforms of the enz...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.11.001
更新日期：2005-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.005
更新日期：2013-01-01 00:00:00
abstract：:Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90128-d
更新日期：1990-09-01 00:00:00
abstract：:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01322-0
更新日期：2002-06-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01279-7
更新日期：2002-01-01 00:00:00
abstract：:We have used a mouse Peyer's patch gut loop model to investigate the role of the intestinal membranous epithelial (M) cells in the pathogenesis of Salmonella typhimurium. These specialized antigen sampling cells are located in the follicle-associated epithelium (FAE) overlying the isolated and aggregated lymphoid foll...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90031-0
更新日期：1994-09-01 00:00:00
abstract：:From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.08.002
更新日期：2003-11-01 00:00:00
abstract：:The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.05.009
更新日期：2010-09-01 00:00:00
abstract：:A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.04.004
更新日期：2007-07-01 00:00:00
abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90031-i
更新日期：1992-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90016-j
更新日期：1990-06-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.09.006
更新日期：2020-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90210-s
更新日期：1993-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90078-8
更新日期：1989-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.09.005
更新日期：2005-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90193-9
更新日期：1989-11-01 00:00:00
abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00148-7
更新日期：2003-10-01 00:00:00
abstract：:Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.010
更新日期：2016-10-01 00:00:00
