Abstract：
:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis of AI-2. In this study, we investigated the AI-2 activity of RA using an AI-2 bioassay, which showed that RA does not produce AI-2. Bioinformatic analysis indicated that the RA genome has a pfs, but not a luxS, homologue. To investigate the function of RA pfs, an avian pathogenic Escherichia coli (APEC) pfs mutant was constructed, which was subsequently transformed with a recombinant plasmid carrying RA pfs. An AI-2 bioassay demonstrated that RA pfs restored AI-2 production to the APEC pfs mutant, suggesting that RA pfs functions in AI-2 synthesis. Furthermore, we found that RA utilizes exogenous AI-2 to regulate biofilm formation. RA biofilm formation decreased significantly upon addition of exogenous AI-2. Real-time quantitative PCR results showed that expression of 13 genes related to RA biofilm formation decreased significantly when exogenous AI-2 was added to the RA culture media. These findings will benefit future studies on AI-2 regulation in RA.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Han X,Liu L,Fan G,Zhang Y,Xu D,Zuo J,Wang S,Wang X,Tian M,Ding C,Yu Sdoi
10.1016/j.resmic.2015.06.004subject
Has Abstractpub_date
2015-07-01 00:00:00pages
486-93issue
6eissn
0923-2508issn
1769-7123pii
S0923-2508(15)00101-1journal_volume
166pub_type
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