Unusual structure of plasmid DNA formed in transformants of Escherichia coli.

Abstract:

:An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and the recA genotype may relate to accumulation of the plasmids conforming to the unusual topology.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Kato M,Furuno A

doi

10.1016/0923-2508(92)90061-r

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

665-9

issue

7

eissn

0923-2508

issn

1769-7123

pii

0923-2508(92)90061-R

journal_volume

143

pub_type

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