Abstract:
:From the nose of a patient with aspiration pneumonia, we isolated a new bacterium by amoebal co-culture using Acanthamoeba polyphaga. Based on phenotypic, 16S rRNA and phylogenetic analysis, we show that this isolate represents a new species within the genus Rhodobacter and and we have named it Rhodobacter massiliensis. The type strain has been deposited in the Collection de l'Institut Pasteur, Paris, France (CIP 107725), and in the Culture Collection of the University of Göteborg, Sweden (CCUG 47968), as strain Framboise.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Greub G,Raoult Ddoi
10.1016/j.resmic.2003.08.002subject
Has Abstractpub_date
2003-11-01 00:00:00pages
631-5issue
9eissn
0923-2508issn
1769-7123pii
S0923-2508(03)00195-5journal_volume
154pub_type
杂志文章abstract::Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.014
更新日期:2009-12-01 00:00:00
abstract::Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(91)90059-j
更新日期:1991-09-01 00:00:00
abstract::The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.01.003
更新日期:2006-09-01 00:00:00
abstract::Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.02.003
更新日期:2012-05-01 00:00:00
abstract::General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2009.08.018
更新日期:2009-11-01 00:00:00
abstract::Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A1 and A2 and discriminated by two levels of DNA/DNA hybridization (delta Tm values of 0 t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90006-v
更新日期:1991-11-01 00:00:00
abstract::Bacterial membranes are known to form domains with specific lipid compositions and functions. Recently, using membrane binding fluorescent dyes, lipid spiral structures extending along the long axis of the cell were detected. These spirals were absent when the synthesis of phosphatidylglycerol and cardiolipin was disr...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.07.006
更新日期:2010-11-01 00:00:00
abstract::The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(99)00130-8
更新日期:1999-11-01 00:00:00
abstract::During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90086-7
更新日期:1989-10-01 00:00:00
abstract::Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(94)90182-1
更新日期:1994-05-01 00:00:00
abstract::There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80003-x
更新日期:1998-07-01 00:00:00
abstract::This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01322-0
更新日期:2002-06-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80020-5
更新日期:1998-11-01 00:00:00
abstract::Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony sprea...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.02.003
更新日期:2008-05-01 00:00:00
abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90193-9
更新日期:1989-11-01 00:00:00
abstract::Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were func...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.04.011
更新日期:2018-01-01 00:00:00
abstract::Explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) provide a challenge in terms of bioremediation. In the present study, sheep rumen was studied for its potential to detoxify HMX using analytical chemistry and molecular microbial ecology tools. Results indicated significant loss (p < 0.05) of HM...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.08.001
更新日期:2012-09-01 00:00:00
abstract::Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01328-1
更新日期:2002-07-01 00:00:00
abstract::The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2003.11.003
更新日期:2004-04-01 00:00:00
abstract::Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.03.004
更新日期:2004-09-01 00:00:00
abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80894-1
更新日期:1995-02-01 00:00:00
abstract::Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.07.006
更新日期:2008-11-01 00:00:00
abstract::Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.05.002
更新日期:2011-09-01 00:00:00
abstract::The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)00008-6
更新日期:2003-01-01 00:00:00
abstract::There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, n...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)00222-9
更新日期:2000-09-01 00:00:00
abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90061-r
更新日期:1992-09-01 00:00:00
abstract::Microbial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one - the arsenivorans strain of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.09.006
更新日期:2020-01-01 00:00:00
abstract::Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.03.003
更新日期:2009-04-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80565-1
更新日期:1995-09-01 00:00:00
abstract::The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or externa...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90024-7
更新日期:1989-05-01 00:00:00