Abstract:
:Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were treated with decoyinine. The genes have been designated dci (for decoyinine-inducible) and gsi (for glucose-starvation-inducible). Using lacZ transcriptional fusions, the dependence of dci and gsi expression on gene products of the sensor and activator classes of bacterial two-component regulatory systems was examined. Transcription of dciA was impaired by a mutation in spoOA, while expression of gsiA was dependent on the early competence genes comP and comA. The implications of these findings are discussed, and a provisional scheme for information flow during the transition phase from growth to sporulation is proposed.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Mueller JP,Mathiopoulos C,Slack FJ,Sonenshein ALdoi
10.1016/0923-2508(91)90059-jsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
805-13issue
7-8eissn
0923-2508issn
1769-7123pii
0923-2508(91)90059-Jjournal_volume
142pub_type
杂志文章,评审abstract::The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80304-5
更新日期:1998-04-01 00:00:00
abstract::Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90019-m
更新日期:1990-06-01 00:00:00
abstract::Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.06.002
更新日期:2014-09-01 00:00:00
abstract::The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90059-y
更新日期:1990-07-01 00:00:00
abstract::Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.012
更新日期:2009-12-01 00:00:00
abstract::Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.10.012
更新日期:2009-03-01 00:00:00
abstract::A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2003.11.005
更新日期:2004-03-01 00:00:00
abstract::Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.05.002
更新日期:2011-09-01 00:00:00
abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.11.020
更新日期:2008-03-01 00:00:00
abstract::Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.04.003
更新日期:2009-06-01 00:00:00
abstract::The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.05.009
更新日期:2010-09-01 00:00:00
abstract::Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90063-9
更新日期:1994-01-01 00:00:00
abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80894-1
更新日期:1995-02-01 00:00:00
abstract::Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.03.001
更新日期:2005-06-01 00:00:00
abstract::In this study, the occurrence and diversity of integrons were evaluated in 697 isolates belonging to Enterobacteriaceae and Aeromonas spp. isolated from urban wastewaters. Screening of integrons was performed by dot blot hybridization and intI-positive strains were further characterized. The global prevalence of integ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.10.010
更新日期:2012-02-01 00:00:00
abstract::A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.04.004
更新日期:2007-07-01 00:00:00
abstract::Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.01.008
更新日期:2013-05-01 00:00:00
abstract::Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.07.003
更新日期:2010-10-01 00:00:00
abstract::In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90016-j
更新日期:1990-06-01 00:00:00
abstract::Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.10.007
更新日期:2012-01-01 00:00:00
abstract::We have used a mouse Peyer's patch gut loop model to investigate the role of the intestinal membranous epithelial (M) cells in the pathogenesis of Salmonella typhimurium. These specialized antigen sampling cells are located in the follicle-associated epithelium (FAE) overlying the isolated and aggregated lymphoid foll...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90031-0
更新日期:1994-09-01 00:00:00
abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80037-0
更新日期:1999-04-01 00:00:00
abstract::During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90086-7
更新日期:1989-10-01 00:00:00
abstract::Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01313-x
更新日期:2002-05-01 00:00:00
abstract::The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.06.005
更新日期:2016-09-01 00:00:00
abstract::Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90031-i
更新日期:1992-01-01 00:00:00
abstract::The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciproflo...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.013
更新日期:2009-12-01 00:00:00
abstract::Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.009
更新日期:2016-10-01 00:00:00
abstract::Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)00168-6
更新日期:2000-07-01 00:00:00