Abstract:
:Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were treated with decoyinine. The genes have been designated dci (for decoyinine-inducible) and gsi (for glucose-starvation-inducible). Using lacZ transcriptional fusions, the dependence of dci and gsi expression on gene products of the sensor and activator classes of bacterial two-component regulatory systems was examined. Transcription of dciA was impaired by a mutation in spoOA, while expression of gsiA was dependent on the early competence genes comP and comA. The implications of these findings are discussed, and a provisional scheme for information flow during the transition phase from growth to sporulation is proposed.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Mueller JP,Mathiopoulos C,Slack FJ,Sonenshein ALdoi
10.1016/0923-2508(91)90059-jsubject
Has Abstractpub_date
1991-09-01 00:00:00pages
805-13issue
7-8eissn
0923-2508issn
1769-7123pii
0923-2508(91)90059-Jjournal_volume
142pub_type
杂志文章,评审abstract::A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00077-9
更新日期:2003-06-01 00:00:00
abstract::The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(97)83624-8
更新日期:1998-01-01 00:00:00
abstract::A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2015.01.003
更新日期:2015-02-01 00:00:00
abstract::The use of plasmid pKK3535 (a pBR322-derived plasmid containing a ribosomal RNA operon) is described for determining rRNA gene restriction patterns. The plasmid allowed the use of both radioactive as well as nonradioactive procedures and provided features that are necessary for the inclusion of internal and/or externa...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90024-7
更新日期:1989-05-01 00:00:00
abstract::Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.08.022
更新日期:2009-12-01 00:00:00
abstract::FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.02.001
更新日期:2018-04-01 00:00:00
abstract::Thirty mosquitocidal strains of Bacillus sphaericus isolated from different sources and localities in Brazil were characterized phenotypically and genetically to determine their relationship. Among the strains tested, 93.3% were shown to be resistant to lincomycin, 96.6% to novobiocin, 60% to chloramphenicol and all s...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80032-1
更新日期:1999-03-01 00:00:00
abstract::Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90082-2
更新日期:1990-11-01 00:00:00
abstract::The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.014
更新日期:2007-04-01 00:00:00
abstract::ATP binding cassette (ABC) systems constitute one of the most abundant superfamilies of proteins. They are involved not only in the transport of a wide variety of substances, but also in many cellular processes and in their regulation. In this paper, we made a comparative analysis of the properties of ABC systems and ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01194-9
更新日期:2001-04-01 00:00:00
abstract::A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90185-d
更新日期:1991-06-01 00:00:00
abstract::In the genus Pseudomonas, two main lineages and 19 phylogenetic groups or subgroups have been defined based on DNA sequence studies of 16S rRNA, gyrB and rpoD genes. In the present study, 33 strains previously classified as Pseudomonas putida were phylogenetically affiliated with their closest relatives in the genus b...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.01.009
更新日期:2013-05-01 00:00:00
abstract::Benzene is biodegraded in the absence of oxygen under a variety of terminal electron-accepting conditions. However, the mechanism by which anaerobic benzene degradation occurs is unclear. Phenol and benzoate have been consistently detected as intermediates of anaerobic benzene degradation, suggesting that the hydroxyl...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01378-5
更新日期:2002-12-01 00:00:00
abstract::Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.03.004
更新日期:2010-05-01 00:00:00
abstract::The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)00008-6
更新日期:2003-01-01 00:00:00
abstract::Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.04.003
更新日期:2009-06-01 00:00:00
abstract::Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z39:-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.01.006
更新日期:2005-05-01 00:00:00
abstract::Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01373-6
更新日期:2002-11-01 00:00:00
abstract::Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A1 and A2 and discriminated by two levels of DNA/DNA hybridization (delta Tm values of 0 t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90006-v
更新日期:1991-11-01 00:00:00
abstract::This paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of L. monocyto...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.03.004
更新日期:2019-01-01 00:00:00
abstract::Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.05.002
更新日期:2011-09-01 00:00:00
abstract::Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.011
更新日期:2007-04-01 00:00:00
abstract::A collection of 57 strains of hyperthermophilic Archaea from the order Thermococcales was screened for the presence of plasmids; 9 plasmids present in six of these strains were isolated and characterized in terms of size and cross-hybridization. The Notl macrorestriction patterns of genomic DNA of strains harbouring t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(97)82452-7
更新日期:1997-12-01 00:00:00
abstract::Since Streptococcus mutans is the principal etiologic agent causing dental caries, by encompassing an array of unique virulence traits, emerging treatment strategies that specifically target the virulence of this pathogen may be promising as alternative approaches compared to conventional antibiotic therapy. In this p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.03.004
更新日期:2014-05-01 00:00:00
abstract::The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)01163-3
更新日期:2001-01-01 00:00:00
abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80894-1
更新日期:1995-02-01 00:00:00
abstract::A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.01.001
更新日期:2010-03-01 00:00:00
abstract::Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00069-X
更新日期:2003-05-01 00:00:00
abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.06.002
更新日期:2013-10-01 00:00:00
abstract::Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.04.008
更新日期:2016-09-01 00:00:00