Abstract：
:Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were functionally important during cold shock because of their gradual upregulation during a temperature decrease under our assay conditions. cspE also showed higher expression during alkane degradation and antibiotic exposure. The transcriptional start site of the cspE gene was determined using 5' rapid amplification of complementary DNA ends. Next, promoter analysis using numerous constructed gfp reporter strains containing deleted fragments of cspE upstream regions identified possible 5' untranslated region (UTR) cis-DNA elements that could be involved in modulating cspE expression. Deletion of cspE led to a growth defect and enhanced biofilm formation, but only at a low temperature. Collectively, our findings show the importance of CspE during cold shock, dynamic regulation of cspE expression under various stressful conditions and a possible 5'-UTR cis-DNA element for regulation of cspE expression. These data provide molecular insight into cspE gene expression during cold-shock adaptation in soil bacteria.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Kim J,Ha S,Park Wdoi
10.1016/j.resmic.2018.04.011subject
Has Abstractpub_date
2018-01-01 00:00:00pages
244-253issue
4-5eissn
0923-2508issn
1769-7123pii
S0923-2508(18)30069-Xjournal_volume
169pub_type
杂志文章abstract：:(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title：Research in microbiology
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pub_type： 杂志文章,评审
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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abstract：:DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01145-1
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journal_title：Research in microbiology
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doi：10.1016/s0923-2508(02)01322-0
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00130-8
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90075-y
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.06.009
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2003-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.02.005
更新日期：2015-04-01 00:00:00
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pub_type： 杂志文章,评审
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.01.003
更新日期：2019-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81383-0
更新日期：1996-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.014
更新日期：2007-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90031-5
更新日期：1991-02-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90026-k
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
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更新日期：2013-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.10.004
更新日期：2014-01-01 00:00:00
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journal_title：Research in microbiology
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更新日期：2004-09-01 00:00:00
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journal_title：Research in microbiology
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doi：10.1016/j.resmic.2008.10.012
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80055-2
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2004.01.012
更新日期：2004-06-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.04.003
更新日期：2009-06-01 00:00:00
abstract：:Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.03.003
更新日期：2009-04-01 00:00:00
abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90003-7
更新日期：1992-02-01 00:00:00
abstract：:Ecologists have documented the process of plant succession for centuries, yet the successional patterns exhibited by microbial communities have received relatively little attention. We examine recent work on microbial succession and show how, despite some key differences, studies of plant succession can serve as a tem...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2010.06.002
更新日期：2010-10-01 00:00:00
