Abstract：
:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing Escherichia coli (STEC). Both generic methods detected all stx genes. In tests with the reference isolates, all methods detected stx1 and stx2, seven subtyping methods detected stx2v(EH250), seven detected stx2e and only two detected stx2f. Four of the subtyping protocols identified stx genes in all of the field isolates. The PCR-RFLP protocols gave contradictory results for approximately 20% of the strains tested. The observed limitations of the protocols were shown to be due to nucleotide sequence variation in the region of the PCR primers. One subtyping protocol that detected the virulence-related genes, eae and ehxA, and all stx except for the stx2f gene, was modified by newly designed primers so that it identified all stx genes. This modified protocol provides comprehensive characterization of STEC in a single multiplex reaction.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Ziebell KA,Read SC,Johnson RP,Gyles CLdoi
10.1016/s0923-2508(02)01322-0subject
Has Abstractpub_date
2002-06-01 00:00:00pages
289-300issue
5eissn
0923-2508issn
1769-7123pii
S0923-2508(02)01322-0journal_volume
153pub_type
杂志文章abstract：:In the genus Pseudomonas, two main lineages and 19 phylogenetic groups or subgroups have been defined based on DNA sequence studies of 16S rRNA, gyrB and rpoD genes. In the present study, 33 strains previously classified as Pseudomonas putida were phylogenetically affiliated with their closest relatives in the genus b...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.01.009
更新日期：2013-05-01 00:00:00
abstract：:The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01239-6
更新日期：2001-09-01 00:00:00
abstract：:The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.07.008
更新日期：2007-10-01 00:00:00
abstract：:The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80012-6
更新日期：1998-10-01 00:00:00
abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90089-7
更新日期：1992-06-01 00:00:00
abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z39:-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.01.006
更新日期：2005-05-01 00:00:00
abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90031-i
更新日期：1992-01-01 00:00:00
abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.011
更新日期：2007-04-01 00:00:00
abstract：:Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.08.003
更新日期：2014-10-01 00:00:00
abstract：:Azo dye derivatives of azobenzene constitute the largest group of dyes used in the textile industry and possess recalcitrant chemical groups, such as those of azo and sulphonic acid. Some microorganisms are able to degrade these aromatic compounds. In the present work, decolourisation of culture media containing azo d...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01332-3
更新日期：2002-07-01 00:00:00
abstract：:Magnetotactic bacteria (MTB) are a group of Gram-negative bacteria synthesizing magnetic crystals that allow them aligning along magnetic field lines. They have diverse morphologies including cocci, rods, vibrio, spirilla, and multicellular magnetotactic prokaryotes (MMPs). MMPs are composed of 10-57 cells with peritr...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.012
更新日期：2014-09-01 00:00:00
abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.03.006
更新日期：2018-01-01 00:00:00
abstract：:Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80037-0
更新日期：1999-04-01 00:00:00
abstract：:For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title：Research in microbiology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.resmic.2014.09.007
更新日期：2015-05-01 00:00:00
abstract：:Klebsiella pneumoniae is one of the major Gram-negative bacterial pathogens causing hospital-acquired multidrug-resistant infections, and the antimicrobial treatment options are scarce. The lack of available antimicrobials has prompted the development of alternative strategies for the treatment of these infections. In...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.01.003
更新日期：2019-01-01 00:00:00
abstract：:Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were func...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.04.011
更新日期：2018-01-01 00:00:00
abstract：:The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90194-7
更新日期：1993-06-01 00:00:00
abstract：:Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.05.008
更新日期：2004-11-01 00:00:00
abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.08.022
更新日期：2009-12-01 00:00:00
abstract：:Jarosite precipitates formed in iron oxidising bioreactors have been shown to harbour iron-oxidisers. The aim of this study was to develop an iron oxidising bioprocess where microorganisms are retained solely on biogenic jarosite particles. Based on preliminary experiments using a fluidised-bed bioreactor (FBR), the f...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.06.004
更新日期：2020-01-01 00:00:00
abstract：:The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90073-j
更新日期：1991-09-01 00:00:00
abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.06.002
更新日期：2013-10-01 00:00:00
abstract：:An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90061-r
更新日期：1992-09-01 00:00:00
abstract：:Mollicute evolution has been marked by significant changes in genome structure and use of their genetic information. These include a reduction in their genome G+C content and the use by most mollicutes of the UGA universal stop codon as tryptophan. More striking is the size reduction in their genome which, for some sp...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.09.007
更新日期：2007-12-01 00:00:00
abstract：:Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.12.005
更新日期：2005-05-01 00:00:00
abstract：:A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.11.005
更新日期：2004-03-01 00:00:00
abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.08.001
更新日期：2006-04-01 00:00:00
abstract：:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug developm...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/S0923-2508(02)00013-X
更新日期：2003-03-01 00:00:00
