当前位置： SCI文献检索 > RESEARCH IN MICROBIOLOGY期刊下所有文献 > Identification of a lacZ gene in Vibrio cholerae.

Identification of a lacZ gene in Vibrio cholerae.

Abstract：

:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the gene product was identified as a protein of approximately 110 kDa. Primary sequence comparisons indicated that this V. cholerae gene is homologous to the E. coli lacZ gene. In contrast to the lac loci of other bacteria, no gene that could specify a lactose transport system was detected in the vicinity of the V. cholerae lacZ gene, which may account for the inability of this species to use lactose. In V. cholerae, portions of open reading frames encoding proteins homologous to the Alcaligenes eutrophus chrA and E. coli galR gene products were detected upstream and downstream from the lacZ gene, respectively.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Parsot C

doi

10.1016/0923-2508(92)90031-i

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

27-36

issue

1

eissn

0923-2508

issn

1769-7123

pii

0923-2508(92)90031-I

journal_volume

143

pub_type

杂志文章

相关文献

RESEARCH IN MICROBIOLOGY文献大全
  • The bacterial phosphotransferase system as a potential vehicle for the entry of novel antibiotics.

    abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/0923-2508(92)90089-7

    authors： Parr TR Jr,Saier MH Jr

    更新日期：1992-06-01 00:00:00

  • Isolation and characterization of a diazotrophic, oxalate-oxidizing bacterium from sour grass (Oxalis pes-caprae L.).

    abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2004.10.009

    authors： Sahin N

    更新日期：2005-04-01 00:00:00

  • First description of bla IMP-8 in a Pseudomonas mendocina isolated at the Hospital Infante D. Pedro, Aveiro, Portugal.

    abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2010.03.004

    authors： Santos C,Caetano T,Ferreira S,Mendo S

    更新日期：2010-05-01 00:00:00

  • Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium.

    abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2005.07.006

    authors： Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

    更新日期：2006-04-01 00:00:00

  • Digitized fluorescent RFLP analysis (fRFLP) as a universal method for comparing genomes of culturable dsDNA viruses: application to bacteriophages.

    abstract：:Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2007.06.002

    authors： Merabishvili M,Verhelst R,Glonti T,Chanishvili N,Krylov V,Cuvelier C,Tediashvili M,Vaneechoutte M

    更新日期：2007-09-01 00:00:00

  • The crystal-forming strains of Bacillus laterosporus.

    abstract：:Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(96)84709-7

    authors： Smirnova TA,Minenkova IB,Orlova MV,Lecadet MM,Azizbekyan RR

    更新日期：1996-06-01 00:00:00

  • The (ADP-ribosyl)ation reaction in thermophilic bacteria.

    abstract：:Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cell...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2006.01.004

    authors： Faraone-Mennella MR,De Maio A,Petrella A,Romano M,Favaloro P,Gambacorta A,Lama L,Nicolaus B,Farina B

    更新日期：2006-07-01 00:00:00

  • Inhibition of bacterial cell surface extension by various means causes blocking of macromolecular synthesis.

    abstract：:It has been suggested that, in rod-shaped bacteria, two sites for peptidoglycan assembly exist: one which is responsible for septum formation and the other, for lateral wall extension. The balance between the activities of these two sites enables bacteria to conserve their own morphology during cell growth. The effect...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/S0923-2508(97)81895-5

    authors： Lleò MM,Canepari P,Fontana R,Satta G

    更新日期：1997-01-01 00:00:00

  • Quantitative investigations into the elimination of in vitro-obtained spores of the non-pathogenic Clostridium butyricum strain CNRZ 528, and their persistence in organs of different species following intravenous spore administration.

    abstract：:Before the Clostridium tumour assay can be applied to the diagnosis of cancer, we sought to investigate--within the framework of a biopharmaceutical safety test--the organ persistence of test spores of Clostridium butyricum CNRZ 528. We found that non-pathogenic spores obtained in vitro, like pathogenic native spores,...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(93)90038-4

    authors： Fabricius EM,Schneeweiss U,Schau HP,Schmidt W,Benedix A

    更新日期：1993-11-01 00:00:00

  • Fever and the control of gram-negative bacteria.

    abstract：:Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/0923-2508(94)90182-1

    authors： Green MH,Vermeulen CW

    更新日期：1994-05-01 00:00:00

  • Antibiotic resistance with particular reference to soil microorganisms.

    abstract：:Evidence of increasing resistance to antibiotics in soil and other natural isolates highlights the importance of horizontal transfer of resistance genes in facilitating gene flux in bacteria. Horizontal gene transfer in bacteria is favored by the presence of mobile genetic elements and by the organization of bacterial...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/s0923-2508(01)01215-3

    authors： Nwosu VC

    更新日期：2001-06-01 00:00:00

  • Microbial community profiling of the Chinoike Jigoku ("Blood Pond Hell") hot spring in Beppu, Japan: isolation and characterization of Fe(III)-reducing Sulfolobus sp. strain GA1.

    abstract：:Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2016.04.011

    authors： Masaki Y,Tsutsumi K,Hirano S,Okibe N

    更新日期：2016-09-01 00:00:00

  • Production and consumption of methane in freshwater lake ecosystems.

    abstract：:The atmospheric concentration of methane (CH(4)), a major greenhouse gas, is mainly controlled by the activities of methane-producing (methanogens) and methane-consuming (methanotrophs) microorganisms. Freshwater lakes are identified as one of the main CH(4) sources, as it was estimated that they contribute to 6-16% o...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2011.06.004

    authors： Borrel G,Jézéquel D,Biderre-Petit C,Morel-Desrosiers N,Morel JP,Peyret P,Fonty G,Lehours AC

    更新日期：2011-11-01 00:00:00

  • Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes.

    abstract：:This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(91)90091-n

    authors： Sibold L,Henriquet M,Possot O,Aubert JP

    更新日期：1991-01-01 00:00:00

  • Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.

    abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(90)90059-y

    authors： Koblavi S,Grimont F,Grimont PA

    更新日期：1990-07-01 00:00:00

  • The rrn locus and gyrB genotyping confirm the existence of two clonal groups in strains of Yersinia enterocolitica subspecies palearctica biovar 1A.

    abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2006.11.011

    authors： Gulati PS,Virdi JS

    更新日期：2007-04-01 00:00:00

  • TrwB: an F(1)-ATPase-like molecular motor involved in DNA transport during bacterial conjugation.

    abstract：:The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.resmic.2005.12.002

    authors： Cabezon E,de la Cruz F

    更新日期：2006-05-01 00:00:00

  • Mycotoxin-producing and other fungi isolated from grapes for wine production, with particular emphasis on ochratoxin A.

    abstract：:Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2004.12.005

    authors： Serra R,Braga A,Venâncio A

    更新日期：2005-05-01 00:00:00

  • Identification of Listeria species by a semi-nested polymerase chain reaction.

    abstract：:Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(96)84020-4

    authors： Manzano M,Cocolin L,Ferroni P,Gasparini V,Narduzzi D,Cantoni C,Comi G

    更新日期：1996-10-01 00:00:00

  • rRNA gene restriction patterns as an epidemiological marker in nosocomial outbreaks of Staphylococcus aureus infections.

    abstract：:rRNA gene restriction patterns (ribotyping) were compared with phage typing, serotyping, enterotoxins and exfoliatin production in the analysis of 26 Staphylococcus aureus strains isolated from two different nosocomial outbreaks. Total DNA was cleaved by EcoRI restriction endonuclease. After agarose gel electrophoresi...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(93)90212-k

    authors： Meugnier H,Fernandez MP,Bes M,Brun Y,Bornstein N,Freney J,Fleurette J

    更新日期：1993-01-01 00:00:00

  • Genomic diversity in Helicobacter and related organisms.

    abstract：:The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.resmic.2007.09.006

    authors： Linz B,Schuster SC

    更新日期：2007-12-01 00:00:00

  • From data banks to data bases.

    abstract：:The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...

    journal_title：Research in microbiology

    pub_type： 杂志文章,评审

    doi：10.1016/0923-2508(91)90073-j

    authors： Danchin A,Médigue C,Gascuel O,Soldano H,Hénaut A

    更新日期：1991-09-01 00:00:00

  • Characterization and phylogenetic identification of a species of spherical multicellular magnetotactic prokaryotes that produces both magnetite and greigite crystals.

    abstract：:Magnetotactic bacteria (MTB) are a group of Gram-negative bacteria synthesizing magnetic crystals that allow them aligning along magnetic field lines. They have diverse morphologies including cocci, rods, vibrio, spirilla, and multicellular magnetotactic prokaryotes (MMPs). MMPs are composed of 10-57 cells with peritr...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2014.07.012

    authors： Zhang R,Chen YR,Du HJ,Zhang WY,Pan HM,Xiao T,Wu LF

    更新日期：2014-09-01 00:00:00

  • Urease biogenesis in Streptococcus thermophilus.

    abstract：:Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2005.04.005

    authors： Mora D,Monnet C,Parini C,Guglielmetti S,Mariani A,Pintus P,Molinari F,Daffonchio D,Manachini PL

    更新日期：2005-11-01 00:00:00

  • Regulation of nisin biosynthesis by continuous cultures and by resting cells of Lactococcus lactis subsp. lactis.

    abstract：:Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(92)90075-y

    authors： Meghrous J,Huot E,Quittelier M,Petitdemange H

    更新日期：1992-11-01 00:00:00

  • Downregulation of pre-rRNA processing gene Mamrd1 decreases growth, conidiation and virulence in the entomopathogenic fungus Metarhizium acridum.

    abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2011.05.004

    authors： Cao Y,Li K,Xia Y

    更新日期：2011-09-01 00:00:00

  • Characterization of Mycobacterium paratuberculosis and "wood-pigeon" mycobacteria by isoenzyme profile and selective staining of immunoprecipitates.

    abstract：:Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(90)90019-m

    authors： Thorel MF,Blom-Potar MC,Rastogi N

    更新日期：1990-06-01 00:00:00

  • Legionella gratiana sp. nov. isolated from French spa water.

    abstract：:During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/0923-2508(89)90086-7

    authors： Bornstein N,Marmet D,Surgot M,Nowicki M,Meugnier H,Fleurette J,Ageron E,Grimont F,Grimont PA,Thacker WL

    更新日期：1989-10-01 00:00:00

  • Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil.

    abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/s0923-2508(00)01172-4

    authors： Poly F,Monrozier LJ,Bally R

    更新日期：2001-01-01 00:00:00

  • Transcriptome analysis of salt-stressed Deinococcus radiodurans and characterization of salt-sensitive mutants.

    abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

    journal_title：Research in microbiology

    pub_type： 杂志文章

    doi：10.1016/j.resmic.2013.07.005

    authors： Im S,Joe M,Kim D,Park DH,Lim S

    更新日期：2013-11-01 00:00:00