Abstract：
:Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cells at different growth phases, and evidence of an active system was obtained in all analyzed microorganisms, with comparable enzymatic levels. Immunochemical analyses with polyclonal antibodies against both eukaryotic anti-(ADP-ribose) transferase and anti-poly(ADP-ribose) polymerase revealed, for all tested organisms, an immunosignal localized in the range of molecular masses between 43-53 kD. Several proteins of various molecular masses were found as ADP-ribose acceptors. Reaction product analyses showed mono(ADP-ribose) to be the only synthesized compound.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Faraone-Mennella MR,De Maio A,Petrella A,Romano M,Favaloro P,Gambacorta A,Lama L,Nicolaus B,Farina Bdoi
10.1016/j.resmic.2006.01.004subject
Has Abstractpub_date
2006-07-01 00:00:00pages
531-7issue
6eissn
0923-2508issn
1769-7123pii
S0923-2508(06)00054-4journal_volume
157pub_type
杂志文章abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01279-7
更新日期：2002-01-01 00:00:00
abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.03.004
更新日期：2004-09-01 00:00:00
abstract：:Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.02.003
更新日期：2013-06-01 00:00:00
abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2003.11.007
更新日期：2004-04-01 00:00:00
abstract：:Although this volume is dedicated to honoring François Jacob, I would like my contribution to broaden the context by recalling the background within which the scientists of those times operated. The specific scientific accomplishments of Jacob will certainly be covered by the other contributors who collaborated with h...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.resmic.2014.05.027
更新日期：2014-06-01 00:00:00
abstract：:Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80894-1
更新日期：1995-02-01 00:00:00
abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01328-1
更新日期：2002-07-01 00:00:00
abstract：:Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.06.002
更新日期：2007-09-01 00:00:00
abstract：:We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.03.002
更新日期：2007-06-01 00:00:00
abstract：:Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.10.012
更新日期：2009-03-01 00:00:00
abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00113-X
更新日期：2003-07-01 00:00:00
abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01172-4
更新日期：2001-01-01 00:00:00
abstract：:The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.01.003
更新日期：2006-09-01 00:00:00
abstract：:Scedosporium apiospermum, a recently isolated phenol-degrading hyphomycete, was shown to be able to productively utilise the diaryl ester phenylbenzoate as its sole source of carbon and energy. The characterisation of degradation intermediates together with the detection of the corresponding catabolic enzymes in crude...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80077-1
更新日期：1999-07-01 00:00:00
abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:In heap bioleaching and waste-rock dumps, complex microbial communities exist in the flowing and interstitial liquid phases and mineral surface-associated biofilms, often embedded in extracellular polymeric substances (EPS). Microbial activity in the interstitial phase and mineral ore surface facilitates mineral degra...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.09.002
更新日期：2020-01-01 00:00:00
abstract：:Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.05.008
更新日期：2004-11-01 00:00:00
abstract：:In this review, we synthesise current models and recent comparative genomic studies describing how bacterial species may emerge through adaptation to a new ecological niche and maintain themselves in the same niche over long time periods. We notably consider the impact of genetic exchange with phylogenetically close r...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2015.06.008
更新日期：2015-12-01 00:00:00
abstract：:Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90082-2
更新日期：1990-11-01 00:00:00
abstract：:A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.008
更新日期：2014-09-01 00:00:00
abstract：:Explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) provide a challenge in terms of bioremediation. In the present study, sheep rumen was studied for its potential to detoxify HMX using analytical chemistry and molecular microbial ecology tools. Results indicated significant loss (p < 0.05) of HM...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.08.001
更新日期：2012-09-01 00:00:00
abstract：:Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.04.007
更新日期：2004-07-01 00:00:00
abstract：:Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(99)80064-3
更新日期：1997-11-01 00:00:00
abstract：:Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony sprea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.02.003
更新日期：2008-05-01 00:00:00
abstract：:The development of nascent cell division sites was studied in Escherichia coli strains containing ftsAts and ftsZts mutations in which septal development is arrested after shift to the restrictive temperature. Division sites were studied by measuring positions of plasmolysis bays, a visible marker for periseptal annul...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90047-e
更新日期：1991-02-01 00:00:00
abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)80565-1
更新日期：1995-09-01 00:00:00
abstract：:Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.04.005
更新日期：2005-11-01 00:00:00
abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.08.005
更新日期：2013-11-01 00:00:00
abstract：:Glucose kinase (Glk) from Streptomyces coelicolor was purified, characterized biochemically and kinetically, and compared to Glk from Streptomyces peucetius var. caesius. In both Streptomyces, 96% of the enzyme activity was detected in the cytosolic fraction. The use of a Glk activity gel showed no isoforms of the enz...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.11.001
更新日期：2005-04-01 00:00:00
abstract：:WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80055-2
更新日期：1999-06-01 00:00:00
