Abstract：
:Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony spreading on the surface of soft agar media. This phenomenon seemed to be independent of biofilm-forming ability. It occurred in different culture media and was dependent on temperature. Formation of a giant S. xylosus colony did not involve a biosurfactant. Microscopic observation showed that the front of the giant colony comprised a single layer of spacing cells with more packed cells in the median area. Supplementation of the soft media with DNase I increased S. xylosus colony spreading, indicating that extracellular DNA may be involved in limiting the phenomenon. The ability of S. xylosus to spread on semi-solid surfaces may constitute an advantage for surface colonization.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Dordet-Frisoni E,Gaillard-Martinie B,Talon R,Leroy Sdoi
10.1016/j.resmic.2008.02.003subject
Has Abstractpub_date
2008-05-01 00:00:00pages
263-9issue
4eissn
0923-2508issn
1769-7123pii
S0923-2508(08)00029-6journal_volume
159pub_type
杂志文章abstract：:Several specific primers for the nifH gene were tested with different pure telluric N2-fixing strains. A PolF/PolR primer set provided successful amplification of 19 representative N2-fixing strains. Three restriction enzymes, HaeIII, NdeII and MnlI, chosen for restriction fragment length polymorphism (RFLP) analyses,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01172-4
更新日期：2001-01-01 00:00:00
abstract：:Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.011
更新日期：2007-04-01 00:00:00
abstract：:Riemerella anatipestifer (RA) causes major economic losses to the duck industry. Autoinducer-2 (AI-2) is a quorum-sensing signal that regulates bacterial physiology. The luxS and pfs genes are required for AI-2 synthesis in many bacterial species. pfs encodes Pfs, which functions upstream of LuxS in the biosynthesis o...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.06.004
更新日期：2015-07-01 00:00:00
abstract：:Adhesion to the intestinal mucosa is considered to be one of the main selection criteria of lactic acid bacteria for probiotic use. Adhesive probiotics are, for example, considered to provide better antagonism against pathogenic bacteria when compared to non-adhesive strains. Here a new model is described for studying...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.08.012
更新日期：2005-03-01 00:00:00
abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.07.006
更新日期：2006-04-01 00:00:00
abstract：:Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.05.004
更新日期：2016-10-01 00:00:00
abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.017
更新日期：2013-02-01 00:00:00
abstract：:Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90082-2
更新日期：1990-11-01 00:00:00
abstract：:The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90073-j
更新日期：1991-09-01 00:00:00
abstract：:Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.03.006
更新日期：2018-01-01 00:00:00
abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00024-X
更新日期：2003-04-01 00:00:00
abstract：:Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90075-y
更新日期：1992-11-01 00:00:00
abstract：:Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.03.004
更新日期：2010-05-01 00:00:00
abstract：:A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 d...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90062-0
更新日期：1994-01-01 00:00:00
abstract：:In order to simplify diagnostic susceptibility testing of mycobacteria against thiacetazone, hydroxylamine, p-nitrobenzoic acid and picric acid, we modified the standard procedure by using Middlebrook 7H11 agar in a single quadrant petri dish. This plate can be inoculated and read in parallel with routine susceptibili...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90078-8
更新日期：1989-03-01 00:00:00
abstract：:The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01342-6
更新日期：2002-09-01 00:00:00
abstract：:RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2020.06.001
更新日期：2020-01-01 00:00:00
abstract：:Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.05.008
更新日期：2004-11-01 00:00:00
abstract：:Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.09.003
更新日期：2007-10-01 00:00:00
abstract：:A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90046-6
更新日期：1989-02-01 00:00:00
abstract：:Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90013-2
更新日期：1989-07-01 00:00:00
abstract：:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90078-7
更新日期：1994-10-01 00:00:00
abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90003-7
更新日期：1992-02-01 00:00:00
abstract：:A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.08.004
更新日期：2005-01-01 00:00:00
abstract：:Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.12.005
更新日期：2005-05-01 00:00:00
abstract：:Scedosporium apiospermum, a recently isolated phenol-degrading hyphomycete, was shown to be able to productively utilise the diaryl ester phenylbenzoate as its sole source of carbon and energy. The characterisation of degradation intermediates together with the detection of the corresponding catabolic enzymes in crude...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80077-1
更新日期：1999-07-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:In this study, the use of flow cytometry to analyze phage-mediated killing of Enterobacter aerogenes under varying conditions of temperature and nutrient availability was assessed. Bacteriophage UZ1, specific for an E. aerogenes strain, was applied at a multiplicity of infection (MOI) of 1 and 1000 to a Teflon surface...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.02.007
更新日期：2006-09-01 00:00:00
abstract：:For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title：Research in microbiology
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.resmic.2014.09.007
更新日期：2015-05-01 00:00:00
abstract：:The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.10.004
更新日期：2014-01-01 00:00:00
