Purification and peptidase activity of a bacteriolytic extracellular enzyme from Pseudomonas aeruginosa.

abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...

journal_title：Research in microbiology

authors： Parsot C

更新日期：1992-01-01 00:00:00

abstract：:While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...

journal_title：Research in microbiology

authors： Morange M

更新日期：2014-06-01 00:00:00

abstract：:WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identi...

journal_title：Research in microbiology

authors： Hutter B,Dick T

更新日期：1999-06-01 00:00:00

abstract：:This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, prei...

journal_title：Research in microbiology

authors： Rossano F,Ortega De Luna L,Buommino E,Cusumano V,Losi E,Catania MR

更新日期：1999-01-01 00:00:00

abstract：:We have used a mouse Peyer's patch gut loop model to investigate the role of the intestinal membranous epithelial (M) cells in the pathogenesis of Salmonella typhimurium. These specialized antigen sampling cells are located in the follicle-associated epithelium (FAE) overlying the isolated and aggregated lymphoid foll...

journal_title：Research in microbiology

authors： Clark MA,Jepson MA,Simmons NL,Hirst BH

更新日期：1994-09-01 00:00:00

abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...

journal_title：Research in microbiology

authors： Santi L,Silva WO,Pinto AF,Schrank A,Vainstein MH

更新日期：2009-12-01 00:00:00

abstract：:There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, n...

journal_title：Research in microbiology

authors： Regnault B,Martin-Delautre S,Lejay-Collin M,Lefèvre M,Grimont PA

更新日期：2000-09-01 00:00:00

abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...

journal_title：Research in microbiology

authors： Hong HA,Khaneja R,Tam NM,Cazzato A,Tan S,Urdaci M,Brisson A,Gasbarrini A,Barnes I,Cutting SM

更新日期：2009-03-01 00:00:00

abstract：:Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...

journal_title：Research in microbiology

authors： Feil EJ,Enright MC,Spratt BG

更新日期：2000-07-01 00:00:00

abstract：:Brucella spp. are Gram-negative, facultative intracellular bacteria that cause a zoonotic world-wide disease. As in other Gram-negative bacteria, its S-LPS (smooth lipopolysaccharide) is a major determinant of virulence. The Brucella melitensis 16M LPS O-antigen is a homopolymer of 4-formamido-4,6, dideoxymannose. In ...

journal_title：Research in microbiology

authors： Godfroid F,Cloeckaert A,Taminiau B,Danese I,Tibor A,de Bolle X,Mertens P,Letesson JJ

更新日期：2000-10-01 00:00:00

abstract：:Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing...

journal_title：Research in microbiology

authors： Fickers P,Leclère V,Guez JS,Béchet M,Coucheney F,Joris B,Jacques P

更新日期：2008-07-01 00:00:00

abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...

journal_title：Research in microbiology

authors： Kadouri DE,Shanks RM

更新日期：2013-10-01 00:00:00

abstract：:In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...

journal_title：Research in microbiology

authors： Levine MM,Hone D,Tacket C,Ferreccio C,Cryz S

更新日期：1990-09-01 00:00:00

abstract：:Benzene is biodegraded in the absence of oxygen under a variety of terminal electron-accepting conditions. However, the mechanism by which anaerobic benzene degradation occurs is unclear. Phenol and benzoate have been consistently detected as intermediates of anaerobic benzene degradation, suggesting that the hydroxyl...

journal_title：Research in microbiology

authors： Coates JD,Chakraborty R,McInerney MJ

更新日期：2002-12-01 00:00:00

abstract：:Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...

journal_title：Research in microbiology

authors： Finkenwirth F,Eitinger T

更新日期：2019-01-01 00:00:00

abstract：:Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in t...

journal_title：Research in microbiology

authors： Bengtsson J,Hartmann M,Unterseher M,Vaishampayan P,Abarenkov K,Durso L,Bik EM,Garey JR,Eriksson KM,Nilsson RH

更新日期：2012-07-01 00:00:00

abstract：:Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...

journal_title：Research in microbiology

authors： Lavrov KV,Shemyakina AO,Grechishnikova EG,Novikov AD,Derbikov DD,Kalinina TI,Yanenko AS

更新日期：2018-01-01 00:00:00

abstract：:Tellurite-containing media are widely used for the screening and isolation of Shiga toxin-producing Escherichia coli (STEC) O157:H7, but tellurite resistance among non-O157 STEC is poorly characterized. Therefore, we investigated 202 STEC strains representing 61 different serotypes from humans, animals or food for the...

journal_title：Research in microbiology

authors： Orth D,Grif K,Dierich MP,Würzner R

更新日期：2007-03-01 00:00:00

abstract：:In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...

journal_title：Research in microbiology

authors： Lill R,Kispal G

更新日期：2001-04-01 00:00:00

abstract：:Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...

journal_title：Research in microbiology

authors： Yamaguchi T,Matsumoto M,Sugimoto Y,Soutome S,Oho T

更新日期：2009-12-01 00:00:00

abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...

journal_title：Research in microbiology

authors： Biéler S,Silva F,Belin D

更新日期：2010-10-01 00:00:00

abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...

journal_title：Research in microbiology

authors： Decat E,Van Mechelen E,Saerens B,Vermeulen SJ,Boekhout T,De Blaiser S,Vaneechoutte M,Deschaght P

更新日期：2013-02-01 00:00:00

abstract：:Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...

journal_title：Research in microbiology

authors： Kaper JB,Levine MM

更新日期：1990-09-01 00:00:00

abstract：:Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...

journal_title：Research in microbiology

authors： Shin H,Bandara N,Shin E,Ryu S,Kim KP

更新日期：2011-10-01 00:00:00

abstract：:The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...

journal_title：Research in microbiology

authors： Germani Y,Dauga C,Duval P,Huerre M,Levy M,Pialoux G,Sansonetti P,Grimont PA

更新日期：1997-05-01 00:00:00

abstract：:This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

journal_title：Research in microbiology

authors： Sibold L,Henriquet M,Possot O,Aubert JP

更新日期：1991-01-01 00:00:00

abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...

journal_title：Research in microbiology

authors： Lo YL,Chen CL,Shen L,Chen YC,Wang YH,Lee CC,Wang LC,Chuang CH,Janapatla RP,Chiu CH,Chang HY

更新日期：2018-04-01 00:00:00

abstract：:The expression of the feruloyl esterase gene faeA, the alpha-glucuronidase gene aguA, the endoxylanase gene xlnB, and the beta-xylosidase gene xlnD from Aspergillus niger on xylose was studied in a wild-type strain and in a CreA mutant. A decrease in expression of all four genes was observed with increasing xylose con...

journal_title：Research in microbiology

authors： de Vries RP,Visser J,de Graaff LH

更新日期：1999-05-01 00:00:00

abstract：:Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...

journal_title：Research in microbiology

authors： Cavaglieri L,Orlando J,Rodríguez MI,Chulze S,Etcheverry M

更新日期：2005-06-01 00:00:00

abstract：:Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...

journal_title：Research in microbiology

authors： Llanes C,Couturier M,Asfeld L,Grimont F,Michel-Briand Y

更新日期：1994-01-01 00:00:00