Clinical and field trials with attenuated Salmonella typhi as live oral vaccines and as "carrier" vaccines.

abstract：:Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...

journal_title：Research in microbiology

authors： Ikeda R,Sawamura K

更新日期：2008-11-01 00:00:00

abstract：:A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...

journal_title：Research in microbiology

authors： Barbati S,Grenga L,Luzi G,Paolozzi L,Ghelardini P

更新日期：2010-03-01 00:00:00

abstract：:A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...

journal_title：Research in microbiology

authors： Mellouli L,Ben Ameur-Mehdi R,Sioud S,Salem M,Bejar S

更新日期：2003-06-01 00:00:00

abstract：:Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...

journal_title：Research in microbiology

authors： Loubradou G,Turcq B

更新日期：2000-05-01 00:00:00

abstract：:Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...

journal_title：Research in microbiology

authors： Smirnova TA,Minenkova IB,Orlova MV,Lecadet MM,Azizbekyan RR

更新日期：1996-06-01 00:00:00

abstract：:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...

journal_title：Research in microbiology

authors： von Freiesleben U,Rasmussen KV

更新日期：1992-09-01 00:00:00

abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...

journal_title：Research in microbiology

authors： Briggs CE,Khandekar SS,Jacobson GR

更新日期：1992-02-01 00:00:00

abstract：:In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...

journal_title：Research in microbiology

authors： Piffaretti JC,Soldati L

更新日期：1990-06-01 00:00:00

abstract：:The structure of spiroplasmas in the Chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. A spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the fi...

journal_title：Research in microbiology

authors： Wang W,Chen J,Du K,Xu Z

更新日期：2004-10-01 00:00:00

abstract：:Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...

journal_title：Research in microbiology

authors： Green MH,Vermeulen CW

更新日期：1994-05-01 00:00:00

abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...

journal_title：Research in microbiology

authors： Debruyne L,Samyn E,De Brandt E,Vandenberg O,Heyndrickx M,Vandamme P

更新日期：2008-03-01 00:00:00

abstract：:Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...

journal_title：Research in microbiology

authors： Desplats C,Krisch HM

更新日期：2003-05-01 00:00:00

abstract：:In this review, we synthesise current models and recent comparative genomic studies describing how bacterial species may emerge through adaptation to a new ecological niche and maintain themselves in the same niche over long time periods. We notably consider the impact of genetic exchange with phylogenetically close r...

journal_title：Research in microbiology

authors： Lassalle F,Muller D,Nesme X

更新日期：2015-12-01 00:00:00

abstract：:Microbial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one - the arsenivorans strain of ...

journal_title：Research in microbiology

authors： Lescure T,Joulian C,Charles C,Ben Ali Saanda T,Charron M,Breeze D,Bauda P,Battaglia-Brunet F

更新日期：2020-01-01 00:00:00

abstract：:RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the...

journal_title：Research in microbiology

authors： Yan H,Cheng Y,Wang L,Chen W

更新日期：2020-01-01 00:00:00

abstract：:Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...

journal_title：Research in microbiology

authors： Paffetti D,Barberio C,Casalone E,Cavalieri D,Fani R,Fia G,Mori E,Polsinelli M

更新日期：1995-09-01 00:00:00

abstract：:The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciproflo...

journal_title：Research in microbiology

authors： Mesak LR,Davies J

更新日期：2009-12-01 00:00:00

abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...

journal_title：Research in microbiology

authors： Kupka D,Liljeqvist M,Nurmi P,Puhakka JA,Tuovinen OH,Dopson M

更新日期：2009-12-01 00:00:00

abstract：:The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

journal_title：Research in microbiology

authors： Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

更新日期：2006-04-01 00:00:00

abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...

journal_title：Research in microbiology

authors： Ledgham F,Soscia C,Chakrabarty A,Lazdunski A,Foglino M

更新日期：2003-04-01 00:00:00

abstract：:The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosu...

journal_title：Research in microbiology

authors： Forestier C,De Champs C,Vatoux C,Joly B

更新日期：2001-03-01 00:00:00

abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...

journal_title：Research in microbiology

authors： Xu N,Qian K,Dong Y,Chen Y,Yu Q,Zhang B,Xing L,Li M

更新日期：2014-04-01 00:00:00

abstract：:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of...

journal_title：Research in microbiology

authors： Bui XT,Wolff A,Madsen M,Bang DD

更新日期：2012-01-01 00:00:00

abstract：:Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...

journal_title：Research in microbiology

authors： Prabu SK,Mahadevan A

更新日期：1992-03-01 00:00:00

abstract：:The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...

journal_title：Research in microbiology

authors： Frey J,Cheng X,Monnerat MP,Abdo EM,Krawinkler M,Bölske G,Nicolet J

更新日期：1998-01-01 00:00:00

abstract：:Jarosite precipitates formed in iron oxidising bioreactors have been shown to harbour iron-oxidisers. The aim of this study was to develop an iron oxidising bioprocess where microorganisms are retained solely on biogenic jarosite particles. Based on preliminary experiments using a fluidised-bed bioreactor (FBR), the f...

journal_title：Research in microbiology

authors： Ahoranta S,Hulkkonen H,Salminen T,Kuula P,Puhakka JA,Lakaniemi AM

更新日期：2020-01-01 00:00:00

abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...

journal_title：Research in microbiology

authors： Parr TR Jr,Saier MH Jr

更新日期：1992-06-01 00:00:00

abstract：:This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...

journal_title：Research in microbiology

authors： Le Marrec C,Moreau S,Leret V,Schäffer A,Blanco C,Trautwetter A

更新日期：1998-03-01 00:00:00

abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...

journal_title：Research in microbiology

authors： Bastiaens L,Springael D,Dejonghe W,Wattiau P,Verachtert H,Diels L

更新日期：2001-12-01 00:00:00

abstract：:The response of Acidithiobacillus ferrooxidans ATCC 23270 to copper was analyzed in sulfur-grown cells by using quantitative proteomics. Forty-seven proteins showed altered levels in cells grown in the presence of 50 mM copper sulfate. Of these proteins, 24 were up-regulated and 23 down-regulated. As seen before in fe...

journal_title：Research in microbiology

authors： Almárcegui RJ,Navarro CA,Paradela A,Albar JP,von Bernath D,Jerez CA

更新日期：2014-11-01 00:00:00