Abstract：
:The structure of spiroplasmas in the Chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. A spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the first time the presence of spiroplasmas in the cells of the crab. Propagation of spiroplasmas within the cells presented various forms that could be sorted into three morphological types: rounded, regular helical, and pleiomorphic or intermediate forms. The spiroplasmas appeared as round bodies during the fallow stage of development or under poor conditions. They showed various shapes such as helices, saccate, branched, tadpole-like and tortoise-like structures while growing, and became long and congregated in late stages of development. When spiroplasmas were isolated from chicken eggs and cultured in M1D medium they appeared to undergo similar morphological changes to those in the crab. The spiroplasmas contained chromatin filaments and peripheral ribosome-like granules and were delimited by distinct unitary membranes. Average diameters were calculated at 0.1 to 0.35 microm for rounded forms and 0.1-0.2 microm for helical or long forms, and they varied in length from 3-12 microm.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Wang W,Chen J,Du K,Xu Zdoi
10.1016/j.resmic.2004.04.010subject
Has Abstractpub_date
2004-10-01 00:00:00pages
630-5issue
8eissn
0923-2508issn
1769-7123pii
S0923-2508(04)00108-1journal_volume
155pub_type
杂志文章abstract：:A collection of 57 strains of hyperthermophilic Archaea from the order Thermococcales was screened for the presence of plasmids; 9 plasmids present in six of these strains were isolated and characterized in terms of size and cross-hybridization. The Notl macrorestriction patterns of genomic DNA of strains harbouring t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(97)82452-7
更新日期：1997-12-01 00:00:00
abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01279-7
更新日期：2002-01-01 00:00:00
abstract：:Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90117-7
更新日期：1992-07-01 00:00:00
abstract：:Scedosporium apiospermum, a recently isolated phenol-degrading hyphomycete, was shown to be able to productively utilise the diaryl ester phenylbenzoate as its sole source of carbon and energy. The characterisation of degradation intermediates together with the detection of the corresponding catabolic enzymes in crude...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80077-1
更新日期：1999-07-01 00:00:00
abstract：:Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive wit...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.02.005
更新日期：2015-04-01 00:00:00
abstract：:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(98)80003-x
更新日期：1998-07-01 00:00:00
abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.11.005
更新日期：2018-01-01 00:00:00
abstract：:Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.04.007
更新日期：2004-07-01 00:00:00
abstract：:The gene encoding a lipoprotein of 67 kDa, named P67, was cloned from Mycoplasma sp. bovine group 7 strain PG50 and expressed in Escherichia coli K12. Analysis of the amino acid sequence derived from the DNA sequence of the P67 gene revealed a typical prokaryotic signal peptidase II membrane lipoprotein lipid attachme...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(97)83624-8
更新日期：1998-01-01 00:00:00
abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2003.11.007
更新日期：2004-04-01 00:00:00
abstract：:General and regulated proteolysis in bacteria is crucial for cellular homeostasis and relies on high substrate specificity of the executing AAA+ proteases. Here we summarize the various strategies that tightly control substrate degradation from both sides: the generation of accessible degrons and their specific recogn...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2009.08.018
更新日期：2009-11-01 00:00:00
abstract：:Ribosomal DNA fingerprints from 103 pathogenic Leptospira strains were examined using EcoRI restriction and fragment length polymorphisms of rRNA genes. Sixty-nine new leptospiral ribotypes were described, in addition to 49 previously observed. Except for 5 strains, a good correlation between DNA homology data and rib...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90210-s
更新日期：1993-01-01 00:00:00
abstract：:A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.11.005
更新日期：2004-03-01 00:00:00
abstract：:An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.06.006
更新日期：2004-12-01 00:00:00
abstract：:The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.05.015
更新日期：2010-09-01 00:00:00
abstract：:Lipoic acid is a covalently-bound enzyme cofactor required for central metabolism all three domains of life. In the last 20 years the pathway of lipoic acid synthesis and metabolism has been established in Escherichia coli. Expression of the genes of the lipoic acid biosynthesis pathway was believed to be constitutive...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.04.005
更新日期：2014-07-01 00:00:00
abstract：:Although it seems obvious that fever has some important general adaptive value, it is still not clear by what means this function is manifested. One postulate is that febrile conditions result in the sequestration of soluble iron, effectively starving some pathogens of that essential nutrient. On the basis of our rece...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(94)90182-1
更新日期：1994-05-01 00:00:00
abstract：:Microcin E492 (MccE492) is an antibacterial protein whose activity on target cells requires ManYZ, the inner membrane component of the mannose permease. We show here that MceA, the polypeptide core of MccE492, stably associates with ManYZ both in the presence and in the absence of MceB, the MccE492 immunity protein. T...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.02.003
更新日期：2012-05-01 00:00:00
abstract：:The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)81587-2
更新日期：1997-05-01 00:00:00
abstract：:Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.06.002
更新日期：2014-09-01 00:00:00
abstract：:The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.10.004
更新日期：2014-01-01 00:00:00
abstract：:Energy-coupling factor (ECF) transporters mediate the uptake of micronutrients in prokaryotes. They consist of two ATP-binding-cassette family ATPases, a transmembrane coupling protein (T component) and a substrate-binding membrane protein (S component). ECF transporters for Co2+ and Ni2+ ions have one or two addition...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.06.007
更新日期：2019-01-01 00:00:00
abstract：:Ecologists have documented the process of plant succession for centuries, yet the successional patterns exhibited by microbial communities have received relatively little attention. We examine recent work on microbial succession and show how, despite some key differences, studies of plant succession can serve as a tem...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2010.06.002
更新日期：2010-10-01 00:00:00
abstract：:The expression of the feruloyl esterase gene faeA, the alpha-glucuronidase gene aguA, the endoxylanase gene xlnB, and the beta-xylosidase gene xlnD from Aspergillus niger on xylose was studied in a wild-type strain and in a CreA mutant. A decrease in expression of all four genes was observed with increasing xylose con...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80053-9
更新日期：1999-05-01 00:00:00
abstract：:Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.10.004
更新日期：2010-01-01 00:00:00
abstract：:Quorum sensing is a cell-density-dependent regulatory mechanism which, in Gram-negative bacteria, usually involves the production and detection of N-acyl homoserine lactones (HSLs). In the last four years HSL-dependent quorum sensing has been identified in members of the Burkholderia cepacia complex, and this mini-rev...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2004.01.006
更新日期：2004-05-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:The possibility of using kaolinite-immobilized plasmodium fragments of Physarella oblonga (Berk. & Curt.) Morgan to maintain their metabolic activity was examined. The immobilization process was carried out with 1 mg of plasmodium of P. oblonga entrapped in 10 g of kaolinite. Sodium acetate (1 mM) was used as a metabo...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)00008-6
更新日期：2003-01-01 00:00:00
abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.004
更新日期：2011-09-01 00:00:00
