Abstract:
:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have successfully infected cells, a key step is the efficient removal or inactivation of phages remaining free in the culture medium. We demonstrate here the use of ferrous ammonium sulphate as an effective agent for the inactivation of mycobacteriophage D29 without impairing phage replication in previously infected host bacteria. Using this property, we report the detection of viable Mycobacterium smegmatis, M. bovis BCG and M. tuberculosis using simple low-cost technology. The method is highly sensitive, since it is able to detect 10 colony-forming units of M. smegmatis. It is also rapid, with the detection of M. tuberculosis in sputum specimens within 48 h.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
McNerney R,Wilson SM,Sidhu AM,Harley VS,al Suwaidi Z,Nye PM,Parish T,Stoker NGdoi
10.1016/s0923-2508(98)80003-xsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
487-95issue
7eissn
0923-2508issn
1769-7123pii
S092325089880003Xjournal_volume
149pub_type
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journal_title:Research in microbiology
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journal_title:Research in microbiology
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journal_title:Research in microbiology
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abstract::During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
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journal_title:Research in microbiology
pub_type: 杂志文章
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abstract::Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...
journal_title:Research in microbiology
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journal_title:Research in microbiology
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journal_title:Research in microbiology
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