Abstract：
:Mercury methylation and demethylation processes govern the fate of methylmercury in aquatic ecosystems. Under anoxic conditions, methylation activity is mainly of biological origin and is often the result of sulfate-reducing bacteria. In this study, the use of a luminescent biosensor for screening methylmercury production was validated by exposing the reporter strain to methylating or non-methylating Desulfovibrio strains. The sensitivity of the biosensor to methylmercury was shown to depend on sulfate-reducing bacterial growth conditions. Bioluminescence was measured using 1-10 mM of sulfides. As the sulfide level increased, luminescence decreased by 40-70%, respectively. Nevertheless, assuming an average of 5 mM of sulfide produced during sulfate-reducing growth, a mercury methylation potential of over 4% was detected when using 185 nM of inorganic mercury. Due to technical limitations, mercury speciation has, to date, only been investigated in a small number of bacterial strains, and no consistent phylogenetic distribution has been identified. Here, the biosensor was further used to assess the Hg methylation capacities of an additional 21 strains related to the Desulfobulbaceae. Seven of them were identified as methylmercury producers. Cultivation procedures combined with bacterial biosensors could provide innovative tools to identify new methylator clades amongst the prokaryotes.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Colin Y,Gury J,Monperrus M,Gentes S,Ayala Borda P,Goni-Urriza M,Guyoneaud Rdoi
10.1016/j.resmic.2017.09.005subject
Has Abstractpub_date
2018-01-01 00:00:00pages
44-51issue
1eissn
0923-2508issn
1769-7123pii
S0923-2508(17)30163-8journal_volume
169pub_type
杂志文章abstract：:The effects of tunicamycin, an inhibitor of N-asparagine-linked glycosylation, on the secretion, adhesion and activities of the cellulase complex produced by Clostridium cellulolyticum have been studied. Tunicamycin at 0.1 micrograms/ml slightly inhibited growth on cellobiose. Endoglucanase, p-nitrophenylcellobiosidas...
journal_title：Research in microbiology
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doi：10.1016/s0923-2508(01)01194-9
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journal_title：Research in microbiology
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doi：10.1016/j.resmic.2007.04.004
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journal_title：Research in microbiology
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pub_type： 杂志文章
doi：10.1016/0923-2508(90)90019-m
更新日期：1990-06-01 00:00:00
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journal_title：Research in microbiology
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doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2005.12.002
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)00008-6
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90051-3
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pub_type： 杂志文章
doi：10.1016/0923-2508(91)90091-n
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journal_title：Research in microbiology
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journal_title：Research in microbiology
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90084-n
更新日期：1991-07-01 00:00:00
abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...
journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2003-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2019.06.007
更新日期：2019-01-01 00:00:00
abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...
journal_title：Research in microbiology
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doi：10.1016/j.resmic.2012.10.005
更新日期：2013-01-01 00:00:00
