Abstract：
:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Trench in the Northwest Pacific Ocean. The genome size of PstS-1 was 48,666 bp; its genome displayed a 59.8% G + C content and a total of 79 coding sequences were identified in its genome. The PstS-1 phage belongs to the family Siphoviridae, but its genomic sequence and organization are distinct from those of any other well-known Siphoviridae phage. The mosaic genomic structure of PstS-1 suggests the occurrence of genetic exchange between distinct temperate phages in deep-sea Pseudomonas populations. The PstS-1 genome also harbors three distinct sequence regions corresponding to spacers within a single clustered regularly interspaced short palindromic repeat (CRISPR) locus in the rhizosphere-associated diazotrophic P. stutzeri A1501 genome. The extension of these spacers to the soil environment and the presence of many homologs of both the hadal deep-sea phage PstS-1 and terrestrial Pseudomonas phages suggest the early co-evolution of temperate phages and their host genus Pseudomonas prior to the divergence of their habitational and physiological adaptation.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai Kdoi
10.1016/j.resmic.2015.05.001subject
Has Abstractpub_date
2015-11-01 00:00:00pages
668-76issue
9eissn
0923-2508issn
1769-7123pii
S0923-2508(15)00076-5journal_volume
166pub_type
杂志文章abstract：:When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
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