Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation.

abstract：:The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...

journal_title：Research in microbiology

authors： Lopatina A,Krylenkov V,Severinov K

更新日期：2013-11-01 00:00:00

abstract：:This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

journal_title：Research in microbiology

authors： Sibold L,Henriquet M,Possot O,Aubert JP

更新日期：1991-01-01 00:00:00

abstract：:There is still an urgent requirement for more sensitive, cost-effective methods for detection and susceptibility testing of mycobacteria in clinical samples. We have been investigating a simple bacteriophage-based system which could be used for both purposes. As this depends upon the detection of phages which have suc...

journal_title：Research in microbiology

authors： McNerney R,Wilson SM,Sidhu AM,Harley VS,al Suwaidi Z,Nye PM,Parish T,Stoker NG

更新日期：1998-07-01 00:00:00

abstract：:The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...

journal_title：Research in microbiology

authors： Debruyne L,Samyn E,De Brandt E,Vandenberg O,Heyndrickx M,Vandamme P

更新日期：2008-03-01 00:00:00

abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...

journal_title：Research in microbiology

authors： Colón-González M,Méndez-Ortiz MM,Membrillo-Hernández J

更新日期：2004-09-01 00:00:00

abstract：:Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...

journal_title：Research in microbiology

authors： Merabishvili M,Verhelst R,Glonti T,Chanishvili N,Krylov V,Cuvelier C,Tediashvili M,Vaneechoutte M

更新日期：2007-09-01 00:00:00

abstract：:By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...

journal_title：Research in microbiology

authors： Boye E,Løbner-Olesen A

更新日期：1991-02-01 00:00:00

abstract：:Macroscopic and microscopic measurements of the growth of Trichoderma viride and Rhizopus oligosporus were compared at different values of water activity of the solid medium. The relationship between radial extension rate and hyphal growth rate was found to be dependent on water activity. The observed difference betwe...

journal_title：Research in microbiology

authors： Molin P,Gervais P,Lemière JP,Davet T

更新日期：1992-10-01 00:00:00

abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...

journal_title：Research in microbiology

authors： Ercolini D,Fusco V,Blaiotta G,Coppola S

更新日期：2005-03-01 00:00:00

abstract：:The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...

journal_title：Research in microbiology

authors： Triplett LR,Wedemeyer WJ,Sundin GW

更新日期：2010-09-01 00:00:00

abstract：:OprI, a small outer membrane lipoprotein from Pseudomonas aeruginosa, can be produced in large amounts and anchored at the surface on Escherichia coli cells. A four-time repeated (His-Cys) motif was fused to the C-terminal part of OprI. After induction, E. coli cells harbouring the recombinant oprI gene became more se...

journal_title：Research in microbiology

authors： Bouia A,Kholti A,Saghi M,Cornelis P

更新日期：2001-11-01 00:00:00

abstract：:The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...

journal_title：Research in microbiology

authors： Willems A,East AK,Lawson PA,Collins MD

更新日期：1993-09-01 00:00:00

abstract：:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

journal_title：Research in microbiology

authors： Chai BF,Wang W,Liang AH

更新日期：2006-04-01 00:00:00

abstract：:Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...

journal_title：Research in microbiology

authors： Henry T,Pommier S,Journet L,Bernadac A,Gorvel JP,Lloubès R

更新日期：2004-07-01 00:00:00

abstract：:The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...

journal_title：Research in microbiology

authors： Smith HO,Gwinn ML,Salzberg SL

更新日期：1999-11-01 00:00:00

abstract：:Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...

journal_title：Research in microbiology

authors： Yim G,Spiegelman GB,Davies JE

更新日期：2013-06-01 00:00:00

abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...

journal_title：Research in microbiology

authors： Cloeckaert A,Zygmunt MS,Bézard G,Dubray G

更新日期：1996-05-01 00:00:00

abstract：:Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...

journal_title：Research in microbiology

authors： Thorel MF,Blom-Potar MC,Rastogi N

更新日期：1990-06-01 00:00:00

abstract：:A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...

journal_title：Research in microbiology

authors： Minnan L,Jinli H,Xiaobin W,Huijuan X,Jinzao C,Chuannan L,Fengzhang Z,Liangshu X

更新日期：2005-01-01 00:00:00

abstract：:It has been suggested that, in rod-shaped bacteria, two sites for peptidoglycan assembly exist: one which is responsible for septum formation and the other, for lateral wall extension. The balance between the activities of these two sites enables bacteria to conserve their own morphology during cell growth. The effect...

journal_title：Research in microbiology

authors： Lleò MM,Canepari P,Fontana R,Satta G

更新日期：1997-01-01 00:00:00

abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...

journal_title：Research in microbiology

authors： Koblavi S,Grimont F,Grimont PA

更新日期：1990-07-01 00:00:00

abstract：:In this study, the use of flow cytometry to analyze phage-mediated killing of Enterobacter aerogenes under varying conditions of temperature and nutrient availability was assessed. Bacteriophage UZ1, specific for an E. aerogenes strain, was applied at a multiplicity of infection (MOI) of 1 and 1000 to a Teflon surface...

journal_title：Research in microbiology

authors： Verthé K,Verstraete W

更新日期：2006-09-01 00:00:00

abstract：:When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...

journal_title：Research in microbiology

authors： Payot S,Guedon E,Gelhaye E,Petitdemange H

更新日期：1999-09-01 00:00:00

abstract：:An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...

journal_title：Research in microbiology

authors： Kato M,Furuno A

更新日期：1992-09-01 00:00:00

abstract：:The sequence of a 4.6-kb region of DNA from Corynebacterium glutamicum ATCC 13869 lying upstream from the ftsQ-ftsZ region has been determined. The region contains four genes with high similarity to the murD, ftsW, murG, and murC genes from different microorganisms. The products of these mur genes probably catalyse se...

journal_title：Research in microbiology

authors： Ramos A,Honrubia MP,Vega D,Ayala JA,Bouhss A,Mengin-Lecreulx D,Gil JA

更新日期：2004-04-01 00:00:00

abstract：:An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...

journal_title：Research in microbiology

authors： Matthies C,Gössner A,Acker G,Schramm A,Drake HL

更新日期：2004-12-01 00:00:00

abstract：:This study evaluated two generic polymerase chain reaction (PCR) protocols, and nine subtyping protocols and three PCR-restriction fragment length polymorphism (RFLP) protocols for detection of stx genes. The PCR protocols were evaluated by testing 12 reference isolates and 496 field strains of Shiga toxin-producing E...

journal_title：Research in microbiology

authors： Ziebell KA,Read SC,Johnson RP,Gyles CL

更新日期：2002-06-01 00:00:00

abstract：:Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...

journal_title：Research in microbiology

authors： Decat E,Van Mechelen E,Saerens B,Vermeulen SJ,Boekhout T,De Blaiser S,Vaneechoutte M,Deschaght P

更新日期：2013-02-01 00:00:00

abstract：:The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...

journal_title：Research in microbiology

authors： Matthijs S,Koedam N,Cornelis P,De Greve H

更新日期：2000-12-01 00:00:00

abstract：:Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...

journal_title：Research in microbiology

authors： Kosikowska U,Andrzejczuk S,Plech T,Malm A

更新日期：2016-10-01 00:00:00