Expression, characterization and immunolocalization of translation termination factor eRF3 in the ciliate Euplotes octocarinatus.

Abstract:

:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli can be tested for its ribosome-dependent GTPase activity using an in vitro system. Moreover, polyclonal antibodies against recombinant Eo-eRF3 were used to determine the localization of Eo-eRF3 using immunofluorescence and immunoelectron microscopy, which showed that the Eo-eRF3 is distributed not only around the macronucleus, but also in basal bodies in the cortex of E. octocarinatus cells. The results suggest that Eo-eRF3 may be involved in cytoskeleton organization in addition to its function in translation termination.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Chai BF,Wang W,Liang AH

doi

10.1016/j.resmic.2005.08.001

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

235-40

issue

3

eissn

0923-2508

issn

1769-7123

pii

S0923-2508(05)00187-7

journal_volume

157

pub_type

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