Abstract:
:Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli can be tested for its ribosome-dependent GTPase activity using an in vitro system. Moreover, polyclonal antibodies against recombinant Eo-eRF3 were used to determine the localization of Eo-eRF3 using immunofluorescence and immunoelectron microscopy, which showed that the Eo-eRF3 is distributed not only around the macronucleus, but also in basal bodies in the cortex of E. octocarinatus cells. The results suggest that Eo-eRF3 may be involved in cytoskeleton organization in addition to its function in translation termination.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Chai BF,Wang W,Liang AHdoi
10.1016/j.resmic.2005.08.001subject
Has Abstractpub_date
2006-04-01 00:00:00pages
235-40issue
3eissn
0923-2508issn
1769-7123pii
S0923-2508(05)00187-7journal_volume
157pub_type
杂志文章abstract::Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)00145-5
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abstract::Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90075-y
更新日期:1992-11-01 00:00:00
abstract::Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.07.001
更新日期:2011-10-01 00:00:00
abstract::Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)81064-3
更新日期:1995-10-01 00:00:00
abstract::In this study, the occurrence and diversity of integrons were evaluated in 697 isolates belonging to Enterobacteriaceae and Aeromonas spp. isolated from urban wastewaters. Screening of integrons was performed by dot blot hybridization and intI-positive strains were further characterized. The global prevalence of integ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.10.010
更新日期:2012-02-01 00:00:00
abstract::The atmospheric concentration of methane (CH(4)), a major greenhouse gas, is mainly controlled by the activities of methane-producing (methanogens) and methane-consuming (methanotrophs) microorganisms. Freshwater lakes are identified as one of the main CH(4) sources, as it was estimated that they contribute to 6-16% o...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.06.004
更新日期:2011-11-01 00:00:00
abstract::Cell death induced by adherence of Staphylococcus aureus was observed in Cryptococcus neoformans. To investigate this death mechanism, we examined three factors: actin dynamics using rhodamine-phalloidin staining, accumulation of reactive oxygen species (ROS) via conversion of 2',7'-dichlorofluorescein diacetate to 2'...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.07.006
更新日期:2008-11-01 00:00:00
abstract::Measuring gene expression at the single cell and single molecule level has recently made possible the quantitative measurement of stochasticity of gene expression. This enables identification of the probable sources and roles of noise. Gene expression noise can result in bacterial population heterogeneity, offering sp...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2017.03.009
更新日期:2017-01-01 00:00:00
abstract::Development of effective strategies for treatment of candidiasis and other fungal diseases has posed a challenge, considering the increase in opportunistic fungal infections in HIV-positive and immunocompromised patients. The in vitro antifungal activity of essential oil from Ocimum gratissimum was investigated in ord...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.04.004
更新日期:2004-09-01 00:00:00
abstract::Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.04.007
更新日期:2004-07-01 00:00:00
abstract::The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90060-2
更新日期:1992-09-01 00:00:00
abstract::Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00069-X
更新日期:2003-05-01 00:00:00
abstract::Adhesion to the intestinal mucosa is considered to be one of the main selection criteria of lactic acid bacteria for probiotic use. Adhesive probiotics are, for example, considered to provide better antagonism against pathogenic bacteria when compared to non-adhesive strains. Here a new model is described for studying...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.08.012
更新日期:2005-03-01 00:00:00
abstract::In order to simplify diagnostic susceptibility testing of mycobacteria against thiacetazone, hydroxylamine, p-nitrobenzoic acid and picric acid, we modified the standard procedure by using Middlebrook 7H11 agar in a single quadrant petri dish. This plate can be inoculated and read in parallel with routine susceptibili...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90078-8
更新日期:1989-03-01 00:00:00
abstract::Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony sprea...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.02.003
更新日期:2008-05-01 00:00:00
abstract::Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90026-k
更新日期:1992-03-01 00:00:00
abstract::Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.011
更新日期:2007-04-01 00:00:00
abstract::A variety of pathways for carbon and electron flow in the bacterium Escherichia coli and in other enteric bacteria are differentially expressed depending on whether molecular oxygen is present in the cell environment. This review briefly summarizes the metabolic pathways operative during aerobic versus anaerobic cell ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(94)90092-2
更新日期:1994-06-01 00:00:00
abstract::Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.07.005
更新日期:2008-11-01 00:00:00
abstract::Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.07.005
更新日期:2013-11-01 00:00:00
abstract::Bacterial membranes are known to form domains with specific lipid compositions and functions. Recently, using membrane binding fluorescent dyes, lipid spiral structures extending along the long axis of the cell were detected. These spirals were absent when the synthesis of phosphatidylglycerol and cardiolipin was disr...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.07.006
更新日期:2010-11-01 00:00:00
abstract::A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90185-d
更新日期:1991-06-01 00:00:00
abstract::The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.01.003
更新日期:2006-09-01 00:00:00
abstract::The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01342-6
更新日期:2002-09-01 00:00:00
abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80894-1
更新日期:1995-02-01 00:00:00
abstract::The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)81383-0
更新日期:1996-05-01 00:00:00
abstract::Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(92)90117-7
更新日期:1992-07-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80020-5
更新日期:1998-11-01 00:00:00
abstract::The phylogenetic diversity of a sediment microbial community from two ponds having different salinities, 150-200 g/l (M2) and 250-300 g/l (TS38), of an Sfax (Tunisia) solar saltern, was investigated using 16S rRNA clone libraries. The 16S rRNA genes from 135 bacterial clones and 105 archaeal clones were sequenced and ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.05.009
更新日期:2010-09-01 00:00:00
abstract::Three Salmonella strains isolated from a lizard (Gallotia simoni) in the "Isla del Hierro" (Canary Islands, Spain) were serotyped as Salmonella bongori serotype 13,22:z39:-, which has not been described in the Kauffmann-White scheme of Salmonella serovars. In order to shed light on the assignment of those strains to t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.01.006
更新日期:2005-05-01 00:00:00