Abstract:
:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytometry to reveal effects on the synchrony of initiations of DNA replication in single cells. An increase in the level of supercoiling moderately reduced initiation synchrony. In contrast, decreased supercoiling led to pronounced asynchrony. We have excluded the possibility that this asynchrony is caused by changes in the level of the Dam methyltransferase or the DnaA protein. We suggest that the global level of supercoiling influences the topology of oriC and thereby the sequence of events leading to initiation of DNA replication in E. coli.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
von Freiesleben U,Rasmussen KVdoi
10.1016/0923-2508(92)90060-2subject
Has Abstractpub_date
1992-09-01 00:00:00pages
655-63issue
7eissn
0923-2508issn
1769-7123pii
0923-2508(92)90060-2journal_volume
143pub_type
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pub_type: 杂志文章,评审
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