The level of supercoiling affects the regulation of DNA replication in Escherichia coli.

Abstract:

:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytometry to reveal effects on the synchrony of initiations of DNA replication in single cells. An increase in the level of supercoiling moderately reduced initiation synchrony. In contrast, decreased supercoiling led to pronounced asynchrony. We have excluded the possibility that this asynchrony is caused by changes in the level of the Dam methyltransferase or the DnaA protein. We suggest that the global level of supercoiling influences the topology of oriC and thereby the sequence of events leading to initiation of DNA replication in E. coli.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

von Freiesleben U,Rasmussen KV

doi

10.1016/0923-2508(92)90060-2

subject

Has Abstract

pub_date

1992-09-01 00:00:00

pages

655-63

issue

7

eissn

0923-2508

issn

1769-7123

pii

0923-2508(92)90060-2

journal_volume

143

pub_type

杂志文章
  • Inhibitory effect of 1,2,4-triazole-ciprofloxacin hybrids on Haemophilus parainfluenzae and Haemophilus influenzae biofilm formation in vitro under stationary conditions.

    abstract::Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2016.05.009

    authors: Kosikowska U,Andrzejczuk S,Plech T,Malm A

    更新日期:2016-10-01 00:00:00

  • Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei sp. nov., a novel marine bacterium isolated from the Andaman Sea.

    abstract::A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2014.07.008

    authors: Poddar A,Lepcha RT,Mukherjee D,Bhattacharyya D,Das SK

    更新日期:2014-09-01 00:00:00

  • Identification of Klebsiella oxytoca using a specific PCR assay targeting the polygalacturonase pehX gene.

    abstract::Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/S0923-2508(03)00148-7

    authors: Kovtunovych G,Lytvynenko T,Negrutska V,Lar O,Brisse S,Kozyrovska N

    更新日期:2003-10-01 00:00:00

  • Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes.

    abstract::This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(91)90091-n

    authors: Sibold L,Henriquet M,Possot O,Aubert JP

    更新日期:1991-01-01 00:00:00

  • Protein-based inheritance in Saccharomyces cerevisiae: [URE3] as a prion form of the nitrogen regulatory protein Ure2.

    abstract::The [URE3] element of the yeast Saccharomyces cerevisiae results from the presence of an altered form of the nitrogen regulatory protein Ure2. This altered form acts as an infectious protein (prion). Genes affecting [URE3] initiation and propagation should give valuable information about prion diseases as well as othe...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(01)01239-6

    authors: Edskes HK

    更新日期:2001-09-01 00:00:00

  • A triclosan-ciprofloxacin cross-resistant mutant strain of Staphylococcus aureus displays an alteration in the expression of several cell membrane structural and functional genes.

    abstract::Triclosan is an antimicrobial agent found in many consumer products. Triclosan inhibits the bacterial fatty acid biosynthetic enzyme, enoyl-ACP reductase (FabI). Decreased susceptibility to triclosan correlates with ciprofloxacin resistance in several bacteria. In these bacteria, resistance to both drugs maps to genes...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2007.09.003

    authors: Tkachenko O,Shepard J,Aris VM,Joy A,Bello A,Londono I,Marku J,Soteropoulos P,Peteroy-Kelly MA

    更新日期:2007-10-01 00:00:00

  • Recombinant attenuated Vibrio cholerae strains used as live oral vaccines.

    abstract::Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(90)90128-d

    authors: Kaper JB,Levine MM

    更新日期:1990-09-01 00:00:00

  • New cblA gene participates in regulation of cobalt-dependent transcription of nitrile hydratase genes in Rhodococcus rhodochrous.

    abstract::Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2018.03.006

    authors: Lavrov KV,Shemyakina AO,Grechishnikova EG,Novikov AD,Derbikov DD,Kalinina TI,Yanenko AS

    更新日期:2018-01-01 00:00:00

  • Analysis of a myosin-like protein and the role of calcium in the E. coli cell cycle.

    abstract::For a number of years now, we have argued that current models for the control of initiation of DNA synthesis, chromosomal partitioning and septum formation in Escherichia coli are unsatisfactory. Indeed, we could argue that despite considerable efforts, with the possible exception of dnaA and ftsZ, no genes specifical...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(91)90031-5

    authors: Casaregola S,Chen M,Bouquin N,Norris V,Jacq A,Goldberg M,Margarson S,Tempete M,Mckenna S,Sweetman H

    更新日期:1991-02-01 00:00:00

  • Identification of a methicillin-resistant Staphylococcus aureus inhibitory compound isolated from Serratia marcescens.

    abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2013.06.002

    authors: Kadouri DE,Shanks RM

    更新日期:2013-10-01 00:00:00

  • Sequence heterogeneity in the lacSZ operon of Streptococcus thermophilus and its use in PCR systems for strain differentiation.

    abstract::Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2004.09.005

    authors: Ercolini D,Fusco V,Blaiotta G,Coppola S

    更新日期:2005-03-01 00:00:00

  • Monitoring of Bordetella isolates circulating in Saint Petersburg, Russia between 2001 and 2009.

    abstract::Bordetella isolates in the Saint Petersburg region have been monitored since 1998. Over the past ten years, concomitant with the increase in pertussis whole-cell vaccine coverage, the incidence of whooping cough has decreased. However, this decrease exists only for Bordetella pertussis infections, as the incidence of ...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2010.09.013

    authors: Kurova N,Njamkepo E,Brun D,Tseneva G,Guiso N

    更新日期:2010-12-01 00:00:00

  • Evolutionary processes and environmental factors underlying the genetic diversity and lifestyles of Bacillus cereus group bacteria.

    abstract::Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resmic.2016.07.002

    authors: Patiño-Navarrete R,Sanchis V

    更新日期:2017-05-01 00:00:00

  • Microbial community profiling of the Chinoike Jigoku ("Blood Pond Hell") hot spring in Beppu, Japan: isolation and characterization of Fe(III)-reducing Sulfolobus sp. strain GA1.

    abstract::Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2016.04.011

    authors: Masaki Y,Tsutsumi K,Hirano S,Okibe N

    更新日期:2016-09-01 00:00:00

  • Mesophilic Aeromonas strains from different serogroups: the influence of growth temperature and osmolarity on lipopolysaccharide and virulence.

    abstract::Growth of mesophilic Aeromonas sp. strains from serogroups O:13, O:33 and O:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (LPS) and virulence of the strains tested, as we had previously reported for strains from serogroup O:34. The effect of osmolarity could be observed when...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(98)80323-9

    authors: Merino S,Aguilar A,Rubires X,Tomás JM

    更新日期:1998-06-01 00:00:00

  • Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium.

    abstract::The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.07.006

    authors: Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

    更新日期:2006-04-01 00:00:00

  • Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy.

    abstract::The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/S0923-2508(97)81587-2

    authors: Germani Y,Dauga C,Duval P,Huerre M,Levy M,Pialoux G,Sansonetti P,Grimont PA

    更新日期:1997-05-01 00:00:00

  • Inhibition of bacterial cell surface extension by various means causes blocking of macromolecular synthesis.

    abstract::It has been suggested that, in rod-shaped bacteria, two sites for peptidoglycan assembly exist: one which is responsible for septum formation and the other, for lateral wall extension. The balance between the activities of these two sites enables bacteria to conserve their own morphology during cell growth. The effect...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/S0923-2508(97)81895-5

    authors: Lleò MM,Canepari P,Fontana R,Satta G

    更新日期:1997-01-01 00:00:00

  • Universal ribotyping method using a chemically labelled oligonucleotide probe mixture.

    abstract::Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/S0923-2508(99)80064-3

    authors: Regnault B,Grimont F,Grimont PA

    更新日期:1997-11-01 00:00:00

  • Chitinolytic activity at low temperature of an Antarctic strain (A3) of Verticillium lecanii.

    abstract::The chitinolytic activity of Verticillium cfr. lecanii A3, a strain isolated from continental Antarctica, was compared to those of two selected strains of Trichoderma harzianum. After 72 h of incubation at 25 degrees C in media containing chitin as the sole carbon source, all strains showed the same enzyme activity (c...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(98)80304-5

    authors: Fenice M,Selbmann L,Di Giambattista R,Federici F

    更新日期:1998-04-01 00:00:00

  • Transcriptome analysis of salt-stressed Deinococcus radiodurans and characterization of salt-sensitive mutants.

    abstract::Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2013.07.005

    authors: Im S,Joe M,Kim D,Park DH,Lim S

    更新日期:2013-11-01 00:00:00

  • Isolation and characterization of a high H2-producing strain Klebsiella oxytoca HP1 from a hot spring.

    abstract::A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2004.08.004

    authors: Minnan L,Jinli H,Xiaobin W,Huijuan X,Jinzao C,Chuannan L,Fengzhang Z,Liangshu X

    更新日期:2005-01-01 00:00:00

  • Selection of species-specific DNA probes which detect strain restriction polymorphism in four Bifidobacterium species.

    abstract::Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(96)80271-3

    authors: Mangin I,Bourget N,Simonet JM,Decaris B

    更新日期:1995-01-01 00:00:00

  • Historical and current perspectives on Clostridium botulinum diversity.

    abstract::For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...

    journal_title:Research in microbiology

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.resmic.2014.09.007

    authors: Smith TJ,Hill KK,Raphael BH

    更新日期:2015-05-01 00:00:00

  • Influence of FkpA variants on survival and replication of Cronobacter spp. in human macrophages.

    abstract::Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive wit...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2015.02.005

    authors: Eshwar AK,Tasara T,Stephan R,Lehner A

    更新日期:2015-04-01 00:00:00

  • Isolation and characterisation of mineral-oxidising "Acidibacillus" spp. from mine sites and geothermal environments in different global locations.

    abstract::Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2016.04.008

    authors: Holanda R,Hedrich S,Ňancucheo I,Oliveira G,Grail BM,Johnson DB

    更新日期:2016-09-01 00:00:00

  • Antibiotic resistance with particular reference to soil microorganisms.

    abstract::Evidence of increasing resistance to antibiotics in soil and other natural isolates highlights the importance of horizontal transfer of resistance genes in facilitating gene flux in bacteria. Horizontal gene transfer in bacteria is favored by the presence of mobile genetic elements and by the organization of bacterial...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(01)01215-3

    authors: Nwosu VC

    更新日期:2001-06-01 00:00:00

  • Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation.

    abstract::The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(00)01163-3

    authors: Tenaillon O,Taddei F,Radmian M,Matic I

    更新日期:2001-01-01 00:00:00

  • Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and Fnr regulons.

    abstract::A variety of pathways for carbon and electron flow in the bacterium Escherichia coli and in other enteric bacteria are differentially expressed depending on whether molecular oxygen is present in the cell environment. This review briefly summarizes the metabolic pathways operative during aerobic versus anaerobic cell ...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/0923-2508(94)90092-2

    authors: Gunsalus RP,Park SJ

    更新日期:1994-06-01 00:00:00

  • Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.

    abstract::The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(90)90059-y

    authors: Koblavi S,Grimont F,Grimont PA

    更新日期:1990-07-01 00:00:00