Mesophilic Aeromonas strains from different serogroups: the influence of growth temperature and osmolarity on lipopolysaccharide and virulence.

Abstract:

:Growth of mesophilic Aeromonas sp. strains from serogroups O:13, O:33 and O:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (LPS) and virulence of the strains tested, as we had previously reported for strains from serogroup O:34. The effect of osmolarity could be observed when the cells grew at 37 degrees C but not at 20 degrees C. Purified LPS from cells cultivated at 20 degrees C (high or low osmolarity) or at 37 degrees C at high osmolarity was smooth, whereas the LPS extracted from the cells cultivated on low osmolarity was rough. The smooth strains were resistant to the bactericidal activity of non-immune serum, while the rough strains were sensitive and showed better adhesion to Hep-2 cells than the rough strains. Furthermore, the smooth strains were more virulent for fish and mice than the rough strains. For mesophilic Aeromonas sp. strains from serogroups O:1 to O:44, these changes were not observed, except for serogroups O:13, O:33, O:34 and O:44.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Merino S,Aguilar A,Rubires X,Tomás JM

doi

10.1016/s0923-2508(98)80323-9

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

407-16

issue

6

eissn

0923-2508

issn

1769-7123

pii

S0923-2508(98)80323-9

journal_volume

149

pub_type

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