The bacterial phosphotransferase system as a potential vehicle for the entry of novel antibiotics.


:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the possibility of providing new opportunities for the discovery of important agents. This system offers a vehicle for entry into infecting bacteria and pathways for the initiation of metabolism of such agents. Antimicrobial agents which would use the PTS may be found which are active on both growing and sessile bacterial forms, and due to the lack of a eukaryotic PTS counterpart, such analogues may be expected to be non-toxic to the animal host.


Res Microbiol


Research in microbiology


Parr TR Jr,Saier MH Jr




Has Abstract


1992-06-01 00:00:00














  • Isolation and characterisation of mineral-oxidising "Acidibacillus" spp. from mine sites and geothermal environments in different global locations.

    abstract::Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Holanda R,Hedrich S,Ňancucheo I,Oliveira G,Grail BM,Johnson DB

    更新日期:2016-09-01 00:00:00

  • Homology-based modeling of the Erwinia amylovora type III secretion chaperone DspF used to identify amino acids required for virulence and interaction with the effector DspE.

    abstract::The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Triplett LR,Wedemeyer WJ,Sundin GW

    更新日期:2010-09-01 00:00:00

  • Spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella.

    abstract::Spirochetes have a unique motility system that is characterized by flagellar filaments contained within the outer membrane sheath. Direct evidence using video microscopy has recently been obtained which indicates that these periplasmic flagella (PF) rotate in several spirochetal species. This rotation generates thrust...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Charon NW,Greenberg EP,Koopman MB,Limberger RJ

    更新日期:1992-07-01 00:00:00

  • Digitized fluorescent RFLP analysis (fRFLP) as a universal method for comparing genomes of culturable dsDNA viruses: application to bacteriophages.

    abstract::Identification and classification of bacteriophages remains a cumbersome process even with the use of genotypic approaches, due to the lack of genes present in all phages. Restriction fragment length polymorphism analysis (RFLP) of the viral genome is a universal approach, but RFLP fingerprints obtained on agarose gel...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Merabishvili M,Verhelst R,Glonti T,Chanishvili N,Krylov V,Cuvelier C,Tediashvili M,Vaneechoutte M

    更新日期:2007-09-01 00:00:00

  • Identification of Bacillus subtilis adaptive response genes by subtractive differential hybridization.

    abstract::Subtractive differential hybridization was used to identify genes in Bacillus subtilis that are induced by nutrient limitation. Several transcription units were identified. They exhibited increased transcription when cells were deprived of certain nutrients, such as glucose, ammonium, or phosphate, or when cells were ...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Mueller JP,Mathiopoulos C,Slack FJ,Sonenshein AL

    更新日期:1991-09-01 00:00:00

  • (51)V NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium.

    abstract::(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Žižić M,Miladinović Z,Stanić M,Hadžibrahimović M,Živić M,Zakrzewska J

    更新日期:2016-07-01 00:00:00

  • Gene cloning and characterization of Streptococcus intermedius fimbriae involved in saliva-mediated aggregation.

    abstract::Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Yamaguchi T,Matsumoto M,Sugimoto Y,Soutome S,Oho T

    更新日期:2009-12-01 00:00:00

  • Nucleotide sequence of nifH regions from Methanobacterium ivanovii and Methanosarcina barkeri 227 and characterization of glnB-like genes.

    abstract::This brief note reports the nucleotide sequences of the second nifH region of Methanobacterium ivanovii and of the two nifH regions of Methanosarcina barkeri 227. In the three cases, nifH is followed by two ORF (open reading frames) similar to ORF105 and ORF128 respectively, which were previously found downstream of M...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Sibold L,Henriquet M,Possot O,Aubert JP

    更新日期:1991-01-01 00:00:00

  • Eubacterial diversity of activated biomass from a common effluent treatment plant.

    abstract::A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Kapley A,De Baere T,Purohit HJ

    更新日期:2007-07-01 00:00:00

  • Identification and genomic analysis of temperate Pseudomonas bacteriophage PstS-1 from the Japan trench at a depth of 7000 m.

    abstract::Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Yoshida M,Yoshida-Takashima Y,Nunoura T,Takai K

    更新日期:2015-11-01 00:00:00

  • Multiple fungal enzymes possess cysteine synthase activity in vitro.

    abstract::We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Brzywczy J,Natorff R,Sieńko M,Paszewski A

    更新日期:2007-06-01 00:00:00

  • Breaking free: "protein antibiotics" and phage lysis.

    abstract::Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Bernhardt TG,Wang IN,Struck DK,Young R

    更新日期:2002-10-01 00:00:00

  • From data banks to data bases.

    abstract::The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Danchin A,Médigue C,Gascuel O,Soldano H,Hénaut A

    更新日期:1991-09-01 00:00:00

  • Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor.

    abstract::WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identi...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Hutter B,Dick T

    更新日期:1999-06-01 00:00:00

  • Adaptive response to environmental changes in the fish pathogen Moritella viscosa.

    abstract::The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Tunsjø HS,Paulsen SM,Mikkelsen H,L'abée-Lund TM,Skjerve E,Sørum H

    更新日期:2007-04-01 00:00:00

  • Antiproliferative activity of Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum in peripheral blood mononuclear cells.

    abstract::Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: do Vale CH,de Oliveira Fraga LA,Costa AS,Tavares CA,Martins-Filho OA,de Macedo Farias L,Roque de Carvalho MA

    更新日期:2004-11-01 00:00:00

  • Microaerophilic bacteria transduce energy via oxidative metabolic gearing.

    abstract::Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Ludwig RA

    更新日期:2004-03-01 00:00:00

  • Unusual structure of plasmid DNA formed in transformants of Escherichia coli.

    abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Kato M,Furuno A

    更新日期:1992-09-01 00:00:00

  • Alternative nitrogenase and pseudogenes: unique features of the Paenibacillus riograndensis nitrogen fixation system.

    abstract::Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Fernandes Gde C,Trarbach LJ,de Campos SB,Beneduzi A,Passaglia LM

    更新日期:2014-09-01 00:00:00

  • Microbial community profiling of the Chinoike Jigoku ("Blood Pond Hell") hot spring in Beppu, Japan: isolation and characterization of Fe(III)-reducing Sulfolobus sp. strain GA1.

    abstract::Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Masaki Y,Tsutsumi K,Hirano S,Okibe N

    更新日期:2016-09-01 00:00:00

  • Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigens.

    abstract::Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Kinoshita H,Wakahara N,Watanabe M,Kawasaki T,Matsuo H,Kawai Y,Kitazawa H,Ohnuma S,Miura K,Horii A,Saito T

    更新日期:2008-11-01 00:00:00

  • Second-order selection in bacterial evolution: selection acting on mutation and recombination rates in the course of adaptation.

    abstract::The increase in genetic variability of a population can be selected during adaptation, as demonstrated by the selection of mutator alleles. The dynamics of this phenomenon, named second-order selection, can result in an improved adaptability of bacteria through regulation of all facets of mutation and recombination pr...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Tenaillon O,Taddei F,Radmian M,Matic I

    更新日期:2001-01-01 00:00:00

  • Improved methods for producing outer membrane vesicles in Gram-negative bacteria.

    abstract::Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Henry T,Pommier S,Journet L,Bernadac A,Gorvel JP,Lloubès R

    更新日期:2004-07-01 00:00:00

  • Subinhibitory fluoroquinolone exposure selects for reduced beta-lactam susceptibility in methicillin-resistant Staphylococcus aureus and alterations in the SOS-mediated response.

    abstract::Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Tattevin P,Basuino L,Chambers HF

    更新日期:2009-04-01 00:00:00

  • The LPS localization might explain the lack of protection of LPS-specific antibodies in abortion-causing Chlamydia psittaci infections.

    abstract::Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Salinas J,Sanchez J,Buendia AJ,Souriau A,Rodolakis A,Bernabé A,Cuello F

    更新日期:1994-10-01 00:00:00

  • Omp85, an evolutionarily conserved bacterial protein involved in outer-membrane-protein assembly.

    abstract::The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审


    authors: Voulhoux R,Tommassen J

    更新日期:2004-04-01 00:00:00

  • Expression, characterization and immunolocalization of translation termination factor eRF3 in the ciliate Euplotes octocarinatus.

    abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Chai BF,Wang W,Liang AH

    更新日期:2006-04-01 00:00:00

  • Comparison of molecular epidemiological tools for Branhamella catarrhalis typing.

    abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Denamur E,Picard-Pasquier N,Mura C,Picard B,Orfila J,Krishnamoorthy R

    更新日期:1991-06-01 00:00:00

  • Transcriptome analysis of salt-stressed Deinococcus radiodurans and characterization of salt-sensitive mutants.

    abstract::Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Im S,Joe M,Kim D,Park DH,Lim S

    更新日期:2013-11-01 00:00:00

  • Identification of Klebsiella oxytoca using a specific PCR assay targeting the polygalacturonase pehX gene.

    abstract::Bacteria of the genus Klebsiella are important opportunistic pathogens responsible for nosocomial infections that are increasingly resistant to antimicrobial agents. Distinctive identification of the species K. oxytoca, K. pneumoniae, K. planticola, K. ornithinolytica and K. terrigena is difficult based on phenotypic ...

    journal_title:Research in microbiology

    pub_type: 杂志文章


    authors: Kovtunovych G,Lytvynenko T,Negrutska V,Lar O,Brisse S,Kozyrovska N

    更新日期:2003-10-01 00:00:00