Abstract:
:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs in other bacteria, including Escherichia coli, the enzyme uses acetyl-CoA as a substrate and therefore is functionally a transacetylase. Moreover, the protein is thermolabile and the gene is under regulation of heat shock transcriptional activator σ32. 3. The gene has a SAM-riboswitch, which shuts off transcription by σ-32 as well as by the vegetative σ-70.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Rotem O,Biran D,Ron EZdoi
10.1016/j.resmic.2012.10.005subject
Has Abstractpub_date
2013-01-01 00:00:00pages
12-6issue
1eissn
0923-2508issn
1769-7123pii
S0923-2508(12)00150-7journal_volume
164pub_type
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