Abstract：
:Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity were dependent on the growth phase and culture conditions. The monitoring of urease biogenesis in batch fermentations revealed the onset of enzyme synthesis starting from the mid-exponential growth phase, with a maximum reached during the late exponential phase. Urease activity strongly increased at acidic pH but to a lesser extent when urea and nickel ions were added to the culture medium. When S. thermophilus cells were cultured with pH maintained at a neutral value, urease activity was detectable only in gel with extremely low signals. Evaluation of beta-glucuronidase activity in strain DSM 20617(T) harboring a transcriptional fusion between a DNA fragment containing the putative urease promoter and the gusA reporter evidenced significant expression at neutral pH that strongly increased in an acidic environment. Further experiments carried out on p(ureI)-gusA recombinant strain revealed that expression of ure genes was not affected by carbohydrates, nickel or urea availability. The presence of consistent expression of ure genes at neutral pH and the absence of induction of expression by carbohydrate availability demonstrated that the transcription of ure genes in S. thermophilus is regulated differently compared with that of the closely related S. salivarius. These differences are discussed taking into consideration the different habitats colonized by the two bacterial species.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Mora D,Monnet C,Parini C,Guglielmetti S,Mariani A,Pintus P,Molinari F,Daffonchio D,Manachini PLdoi
10.1016/j.resmic.2005.04.005subject
Has Abstractpub_date
2005-11-01 00:00:00pages
897-903issue
9eissn
0923-2508issn
1769-7123pii
S0923-2508(05)00116-6journal_volume
156pub_type
杂志文章abstract：:Measuring gene expression at the single cell and single molecule level has recently made possible the quantitative measurement of stochasticity of gene expression. This enables identification of the probable sources and roles of noise. Gene expression noise can result in bacterial population heterogeneity, offering sp...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.03.009
更新日期：2017-01-01 00:00:00
abstract：:A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.007
更新日期：2007-03-01 00:00:00
abstract：:The sensitivity of a bacterium to beta-lactam antibiotics depends upon the interplay between 3 independent factors: the sensitivity of the essential penicillin-binding enzyme(s), the quantity and properties of the beta-lactamase(s) and the diffusion barrier that the outer-membrane of Gram-negative bacteria can represe...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90084-n
更新日期：1991-07-01 00:00:00
abstract：:Long polar fimbriae (LPF) are related to type I fimbriae in genetic organization and were first identified in Salmonella enterica serovar Typhimurium. Four lpfA genetic variants designated lpfA(O157/OI-141), lpfA(O157/OI-154), lpfA(O26) and lpfA(O113) have been identified in Shiga toxin-producing Escherichia coli (STE...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.06.009
更新日期：2006-03-01 00:00:00
abstract：:The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90042-6
更新日期：1994-11-01 00:00:00
abstract：:Bacillus laterosporus is a spore-forming bacterium characterized by its ability to produce a canoe-shaped lamellar parasporal inclusion, adjacent to the spore. In some B. laterosporus strains crystalline inclusions of various shapes and sizes, which are released separately from spores during the lysis of the sporangiu...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)84709-7
更新日期：1996-06-01 00:00:00
abstract：:By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(91)90020-b
更新日期：1991-02-01 00:00:00
abstract：:The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90073-j
更新日期：1991-09-01 00:00:00
abstract：:We showed in 1988 that there are two strains of Chlamydia psittaci which infect the koala (Phascolarctos cinereus). In order to further investigate the role of these chlamydial strains in pathogenesis, we have attempted to identify genes of koala type I strain chlamydia which are involved in the immunogenic response. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(97)83872-7
更新日期：1997-06-01 00:00:00
abstract：:Differential immunoproteomics was applied to identify proteins secreted by Metarhizium anisopliae induced by the Rhipicephalus microplus cuticle. In addition, IgG anti-spore surface proteins were used for searching for proteins possibly involved in early stages of fungus versus tick infection. LC-MS/MS of differential...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.09.012
更新日期：2009-12-01 00:00:00
abstract：:Infections caused by Staphylococcus aureus are prevalent. The dramatically reduced discovery of new antibiotics, as well as the persistent emergence of resistant bacteria, represents a major health problem in both hospital and community settings. Using antibiotic enhancers to rescue existing classes of antibiotics is ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.08.002
更新日期：2017-01-01 00:00:00
abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90003-7
更新日期：1992-02-01 00:00:00
abstract：:The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80012-6
更新日期：1998-10-01 00:00:00
abstract：:At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章,评审
doi：10.1016/s0923-2508(00)00116-9
更新日期：2000-03-01 00:00:00
abstract：:Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.05.008
更新日期：2004-11-01 00:00:00
abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.07.005
更新日期：2013-11-01 00:00:00
abstract：:DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus. In t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2017.12.006
更新日期：2018-04-01 00:00:00
abstract：:Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2003.11.001
更新日期：2004-03-01 00:00:00
abstract：:Six thermophilic extremophiles, Anoxybacillus amylolyticus, Geobacillus thermoleovorans, Geobacillus thermoleovorans subspecies stromboliensis, Geobacillus toebii subspecies decanicus, Bacillus thermantarcticus and Thermus oshimai, isolated from different environmental sites, were studied for their heavy metal resista...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.10.012
更新日期：2009-03-01 00:00:00
abstract：:Bacteriophages must destroy the bacterial cell wall to lyse their host and release their progeny into the environment. There are at least two distinct mechanisms by which phages destroy the cell wall. Bacteriophages with large genomes use a holin-endolysin system, while bacteriophages with small genomes encode a singl...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01330-x
更新日期：2002-10-01 00:00:00
abstract：:A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.008
更新日期：2014-09-01 00:00:00
abstract：:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expressio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.02.001
更新日期：2018-04-01 00:00:00
abstract：:Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90026-k
更新日期：1992-03-01 00:00:00
abstract：:As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.11.002
更新日期：2009-03-01 00:00:00
abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81383-0
更新日期：1996-05-01 00:00:00
abstract：:The association between bacteria and leaves in Ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. This intimate interaction suggests that both partners have co-diversified to...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.04.003
更新日期：2011-06-01 00:00:00
abstract：:Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.04.003
更新日期：2009-06-01 00:00:00
abstract：:A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.08.004
更新日期：2005-01-01 00:00:00
abstract：:There are several occasions when enumeration of Escherichia coli cells is needed. These include examination of urine specimens and water or food samples. Present methods rely on growth in more or less selective media (colony-forming units on agar or the most probable number method using liquid media). Unfortunately, n...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)00222-9
更新日期：2000-09-01 00:00:00
abstract：:Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90063-9
更新日期：1994-01-01 00:00:00
