Abstract：
:FliA is known to be a sigma factor that regulates bacterial flagella gene expression. Accumulating evidence suggests that FliA is involved in bacterial behavior other than motility. To elucidate the contribution of FliA to Pseudomonas aeruginosa pathophysiology, we analyzed the biological properties and gene expression profiles of a ΔfliA mutant. Transcriptome analysis results demonstrated that the expression levels of flagella biogenesis genes decreased dramatically in the mutant; consequently, the ΔfliA mutant failed to synthesize flagella and exhibited reduced motility. The ΔfliA mutant displayed stronger hemolytic and caseinolytic activities, as well as pyocyanin production. The expression of type 6 secretion system-II genes and interbacterial competition activity was decreased in the ΔfliA mutant. Direct evidence of fliA participation in virulence was obtained from analysis of hypervirulent strain B136-33. Adhesion to and cytotoxicity toward mammalian cells and penetration through cell layers were noted; furthermore, the colonization ability of the fliA::Tn5 mutant in the intestines of laboratory mice was compromised. Notably, the fliA-overexpressing strain displayed phenotypes similar to that of the fliA-defective strain, indicating that optimal FliA levels are critical to bacterial physiology. Our findings indicate that FliA plays diverse roles in P. aeruginosa, not only in flagella biosynthesis, but also in pathophysiology.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Lo YL,Chen CL,Shen L,Chen YC,Wang YH,Lee CC,Wang LC,Chuang CH,Janapatla RP,Chiu CH,Chang HYdoi
10.1016/j.resmic.2018.02.001subject
Has Abstractpub_date
2018-04-01 00:00:00pages
135-144issue
3eissn
0923-2508issn
1769-7123pii
S0923-2508(18)30013-5journal_volume
169pub_type
杂志文章abstract：:(51)V NMR spectroscopy was used for detection and identification of cell-associated vanadate (V(5+)) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal w...
journal_title：Research in microbiology
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journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.014
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journal_title：Research in microbiology
pub_type： 临床试验,杂志文章,评审
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abstract：:We present evidence that there are at least three Aspergillus nidulans enzymes which catalyze in vitro the reaction of O-acetylserine (OAS) with sulfide forming cysteine. This activity is shared by cysteine synthase (CS) encoded by the cysB gene, homocysteine synthase encoded by cysD and by at least one more enzyme. M...
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doi：10.1016/j.resmic.2007.03.002
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pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.010
更新日期：2016-10-01 00:00:00
abstract：:A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
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pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.011
更新日期：2016-09-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.07.006
更新日期：2006-04-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/0923-2508(91)90059-j
更新日期：1991-09-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90024-7
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.005
更新日期：2013-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2005.01.006
更新日期：2005-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.04.003
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abstract：:Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.05.009
更新日期：2016-10-01 00:00:00
abstract：:The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.07.008
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pub_type： 杂志文章
doi：10.1016/j.resmic.2016.08.002
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
abstract：:Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81064-3
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abstract：:A strain of Bacteroides fragilis, isolated from the marmoset Callithrix penicillata, produced protein(s) with bacteriocin activity (fragicilin). Two active fractions (36 and 150 kDa) were isolated by chromatography. The bacteriocin exhibited iso- and heteroantagonism. It remained stable between pH 3 and 10 and at 60 d...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90062-0
更新日期：1994-01-01 00:00:00
abstract：:Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
abstract：:A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.04.004
更新日期：2007-07-01 00:00:00
abstract：:The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90004-l
更新日期：1993-09-01 00:00:00
