Abstract:
:A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns. When several isolates of a species were tested, the patterns obtained were often identical. However, more than one pattern was often observed when more than one serotype was considered. The method should be useful for the identification of all species of Legionella including those exhibiting immunological cross-reactions.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Grimont F,Lefèvre M,Ageron E,Grimont PAdoi
10.1016/0923-2508(89)90193-9subject
Has Abstractpub_date
1989-11-01 00:00:00pages
615-26issue
9eissn
0923-2508issn
1769-7123pii
0923-2508(89)90193-9journal_volume
140pub_type
杂志文章abstract::When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)00110-2
更新日期:1999-09-01 00:00:00
abstract::Chinoike Jigoku ("Blood Pond Hell") is located in the hot spring town of Beppu on the southern island of Kyushu in Japan, and is the site of a red-colored acidic geothermal pond. This study aimed to investigate the microbial population composition in this extremely acidic environment and to isolate/characterize acidop...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.04.011
更新日期:2016-09-01 00:00:00
abstract::In this review, we synthesise current models and recent comparative genomic studies describing how bacterial species may emerge through adaptation to a new ecological niche and maintain themselves in the same niche over long time periods. We notably consider the impact of genetic exchange with phylogenetically close r...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2015.06.008
更新日期:2015-12-01 00:00:00
abstract::A highly effective chlorpyrifos-degrading bacterium strain Dsp-2 was isolated from the polluted treatment system of a chlorpyrifos manufacturer. This strain was preliminarily identified as Sphingomonas sp. based on its morphological, physiological and biochemical tests as well as 16S rDNA analysis. It utilized chlorpy...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.007
更新日期:2007-03-01 00:00:00
abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::The outer membrane of gram-negative bacteria acts as a barrier against harmful lipophilic compounds and larger molecules unable to diffuse freely through the porins. However, outer membrane proteins together with the Tol-Pal and TonB systems have been exploited for the entry of macromolecules such as bacteriocins and ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01226-8
更新日期:2001-07-01 00:00:00
abstract::A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.07.008
更新日期:2014-09-01 00:00:00
abstract::Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are widely distributed in the natural environment and play crucial roles in the nitrification process and the removal of nitrogen (N). Although planktonic microbial community plays an important role in river biogeochemical cycles, few studies have attempted to address...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.08.003
更新日期:2014-10-01 00:00:00
abstract::Nisin production by Lactococcus lactis subsp. lactis has been investigated using lactose as carbon source. Whether or not continuous cultures were lactose-limited, maximum nisin titre was observed at an intermediate mu value with a sharp peak of activity between 0.2 and 0.3/h. The maximum specific growth rate obtained...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90075-y
更新日期:1992-11-01 00:00:00
abstract::A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2015.01.003
更新日期:2015-02-01 00:00:00
abstract::A hydrogen-producing bacterial strain was newly isolated from a hot spring and identified as Klebsiella oxytoca HP1 by 16S rRNA gene sequence analysis and detection by BioMerieux Vitek. Important parameters, including substrates, starting pH of culture, temperature and oxygen concentration for batch ferment hydrogen p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.08.004
更新日期:2005-01-01 00:00:00
abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01279-7
更新日期:2002-01-01 00:00:00
abstract::Staphylococcus xylosus is a commensal species commonly found on the skin of mammals, but also currently used as starter culture for meat fermentation. Most strains of this species colonize by forming a biofilm on abiotic surfaces. We show here that the majority of S. xylosus strains also exhibit extensive colony sprea...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.02.003
更新日期:2008-05-01 00:00:00
abstract::ATP binding cassette (ABC) systems constitute one of the most abundant superfamilies of proteins. They are involved not only in the transport of a wide variety of substances, but also in many cellular processes and in their regulation. In this paper, we made a comparative analysis of the properties of ABC systems and ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01194-9
更新日期:2001-04-01 00:00:00
abstract::Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.01.008
更新日期:2013-05-01 00:00:00
abstract::Species of Alicyclobacillus, Bacillus and Thermus genera were selected in order to study the possible presence of the (ADP-ribosyl)ation system. These bacteria are thermophilic, aerobic, and were isolated from different geothermal sources. Both activity and expression of (ADP-ribosyl)ating proteins were tested in cell...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.01.004
更新日期:2006-07-01 00:00:00
abstract::A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90046-6
更新日期:1989-02-01 00:00:00
abstract::Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80271-3
更新日期:1995-01-01 00:00:00
abstract::The structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of the fruit tree pathogen Erwinia amylovora, was modeled based on predicted structural homology to characterized T3SS chaperones. This model guided the selection of 11 amino acid residues that were individually mutated to alani...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.05.015
更新日期:2010-09-01 00:00:00
abstract::In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90016-j
更新日期:1990-06-01 00:00:00
abstract::Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)81064-3
更新日期:1995-10-01 00:00:00
abstract::The tricarballylate utilization locus (tcuRABC) of Salmonella enterica serovar Typhimurium is comprised of a 3-gene operon (tcuABC) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcuR gene, which encodes a putative LysR-type transcriptional regulator. ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.01.001
更新日期:2009-04-01 00:00:00
abstract::Streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. To identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. The cloned gene cluster encoding S. intermedius fimbriae, (saliva...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.014
更新日期:2009-12-01 00:00:00
abstract::Microbial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one - the arsenivorans strain of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.09.006
更新日期:2020-01-01 00:00:00
abstract::Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01313-x
更新日期:2002-05-01 00:00:00
abstract::The presence of viral dsRNA (L and M fractions) in the cryophilic yeast Saccharomyces bayanus var. uvarum is documented here for the first time. Sixty-eight strains of different origins were analyzed. Most of them did not carry dsRNA; the L fraction was found in seven strains, while 11 strains had both L and M fractio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.07.008
更新日期:2007-10-01 00:00:00
abstract::The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2007.09.006
更新日期:2007-12-01 00:00:00
abstract::Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.03.006
更新日期:2018-01-01 00:00:00
abstract::Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.10.017
更新日期:2013-02-01 00:00:00
abstract::By employing flow cytometry, the DNA content and cell size of individual bacterial cells may be determined rapidly and with high precision. Also, the number of DNA replication origins in Escherichia coli cells can be measured after treating the cells with rifampicin together with the cell division inhibitor cephalexin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90020-b
更新日期:1991-02-01 00:00:00