Abstract：
:Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling conjugative DNA transfer. When such translocons transport DNA, they require the assistance of multimeric integral inner membrane proteins, the type IV coupling proteins. Its structural prototype is plasmid R388 TrwB protein, responsible for coupling the relaxosome with the DNA transport apparatus during bacterial conjugation. Its monomeric molecular structure is reminiscent of ring helicases and AAA ATPases. The quaternary structure is made up by six equivalent protomers featuring a flattened sphere resembling F1-ATPase, with a central channel traversing the particle, thus connecting cytoplasm and periplasm.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Gomis-Rüth FX,de la Cruz F,Coll Mdoi
10.1016/s0923-2508(02)01313-xsubject
Has Abstractpub_date
2002-05-01 00:00:00pages
199-204issue
4eissn
0923-2508issn
1769-7123pii
S0923-2508(02)01313-Xjournal_volume
153pub_type
杂志文章,评审abstract：:Pathogenic Vibrio cholerae strains were compared by fingerprinting with arbitrarily primed polymerase chain reaction (AP-PCR). They were O1 classical and El Tor strains and recent non-O1 Bengal strains. Ten oligonucleotides from a total of fifty-two tested gave distinctive patterns, and these strains were separated in...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(96)81064-3
更新日期：1995-10-01 00:00:00
abstract：:Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.03.001
更新日期：2014-04-01 00:00:00
abstract：:Monoclonal antibody II-6-18 recognizes a serogroup-1-specific Legionella pneumophila antigenic determinant which has been shown to be virulence-associated. We previously reported the physicochemical characterization by means of a quantitative fluorometric assay of monoclonal antibody II-6-18 binding to L. pneumophila,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90082-2
更新日期：1990-11-01 00:00:00
abstract：:The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01279-7
更新日期：2002-01-01 00:00:00
abstract：:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(90)90059-y
更新日期：1990-07-01 00:00:00
abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2012.10.005
更新日期：2013-01-01 00:00:00
abstract：:The chromosome of Escherichia coli is negatively supercoiled. This favours processes that unwind the two DNA strands, such as DNA replication. In this paper, we have investigated the effect of changed levels of overall chromosomal supercoiling on the initiation of DNA replication. Specifically, we have used flow cytom...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90060-2
更新日期：1992-09-01 00:00:00
abstract：:Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2018.03.006
更新日期：2018-01-01 00:00:00
abstract：:The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.014
更新日期：2007-04-01 00:00:00
abstract：:Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.004
更新日期：2011-09-01 00:00:00
abstract：:Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.008
更新日期：2016-09-01 00:00:00
abstract：:Total genomic DNA isolated by concentrating seawater and mercury-resistant bacteria were hybridized with a mer probe to detect the presence of homologous DNA sequences in marine coastal waters of the Bay of Bengal, India. Coastal water extracts induced with mercury hybridized with the mer operon of transposon Tn501. M...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90026-k
更新日期：1992-03-01 00:00:00
abstract：:Quorum sensing is a cell-density-dependent regulatory mechanism which, in Gram-negative bacteria, usually involves the production and detection of N-acyl homoserine lactones (HSLs). In the last four years HSL-dependent quorum sensing has been identified in members of the Burkholderia cepacia complex, and this mini-rev...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2004.01.006
更新日期：2004-05-01 00:00:00
abstract：:In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.06.002
更新日期：2013-10-01 00:00:00
abstract：:An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.06.006
更新日期：2004-12-01 00:00:00
abstract：:Mesophilic iron and sulfur-oxidizing acidophiles are readily found in acid mine drainage sites and bioleaching operations, but relatively little is known about their activities at suboptimal temperatures and in cold environments. The purpose of this work was to characterize the oxidation of elemental sulfur (S(0)), te...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.08.022
更新日期：2009-12-01 00:00:00
abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.07.005
更新日期：2013-11-01 00:00:00
abstract：:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.10.009
更新日期：2005-04-01 00:00:00
abstract：:Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.05.004
更新日期：2016-10-01 00:00:00
abstract：:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(94)90078-7
更新日期：1994-10-01 00:00:00
abstract：:During the past twenty-nine years, not a single class of antimicrobial agents has been discovered that has led to new approved human drugs. Despite a dramatic increase in the potency of existing classes, the need for new effective antimicrobial agents continues. The bacterial phosphotransferase system (PTS) offers the...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(92)90089-7
更新日期：1992-06-01 00:00:00
abstract：:Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00069-X
更新日期：2003-05-01 00:00:00
abstract：:WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80055-2
更新日期：1999-06-01 00:00:00
abstract：:Sequence analysis of the 16S ribosomal RNA gene was used to study bacterial diversity of a pristine forest soil and of two cultures of the same soil enriched with cellulolytic bacteria. Our analysis revealed high bacterial diversity in the native soil sample, evidencing at least 10 phyla, in which Actinobacteria, Prot...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.12.001
更新日期：2012-04-01 00:00:00
abstract：:The promoter probe mini-Tn5-luxAB-tet was used to create a luxAB transcriptional fusion responding to fluorene in the fluorene utilising bacterium Sphingomonas sp. LB126. The mutant strain, named L-132, was impaired in fluorene utilisation and strongly emitted light upon addition of fluorene to the growth medium. L-13...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(01)01268-2
更新日期：2001-12-01 00:00:00
abstract：:5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(93)90048-7
更新日期：1993-03-01 00:00:00
abstract：:Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80020-5
更新日期：1998-11-01 00:00:00
abstract：:Legume-nodulating rhizobia use N-acyl homoserine lactones (AHLs) to regulate several physiological traits related to the symbiotic plant-microbe interaction. In this work, we show that Sinorhizobium fredii SMH12, Rhizobium etli ISP42 and Rhizobium sullae IS123, three rhizobial strains with different nodulation ranges,...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90086-7
更新日期：1989-10-01 00:00:00
abstract：:A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.04.004
更新日期：2007-07-01 00:00:00
