Abstract:
:A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed at 37 and 42 degrees C with oxalate and in the presence of 3-4% NaCl and 2% potassium oxalate. In liquid culture, the doubling time (t(d)) with oxalate was 9 h. Its closest phylogenetic neighbors, as deduced by 16S rDNA-based analysis, were Azospirillum brasilense, Azospirillum doebereinerae and Azospirillum lipoferum, with 99.5, 98.4 and 96.7% sequence similarity, respectively. The strain differed from A. brasilense by its ability to use N-acetylglucosamine, D-glucose and D-mannitol. It may be a variant strain of A. brasilense. Oxalotrophic, N2-fixing species of the genus Azospirillum may be important contributors to soil formation, soil fertility, and retention and/or cycling of elements necessary for plant growth.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Sahin Ndoi
10.1016/j.resmic.2004.10.009subject
Has Abstractpub_date
2005-04-01 00:00:00pages
452-6issue
3eissn
0923-2508issn
1769-7123pii
S0923-2508(04)00271-2journal_volume
156pub_type
杂志文章abstract::Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.10.004
更新日期:2010-01-01 00:00:00
abstract::Growth of mesophilic Aeromonas sp. strains from serogroups O:13, O:33 and O:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (LPS) and virulence of the strains tested, as we had previously reported for strains from serogroup O:34. The effect of osmolarity could be observed when...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80323-9
更新日期:1998-06-01 00:00:00
abstract::Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00069-X
更新日期:2003-05-01 00:00:00
abstract::Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation. ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(99)00120-5
更新日期:1999-11-01 00:00:00
abstract::Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.10.017
更新日期:2013-02-01 00:00:00
abstract::The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.10.004
更新日期:2014-01-01 00:00:00
abstract::The human gastric pathogen Helicobacter pylori possesses an enormous genomic plasticity and diversity that facilitates host adaptation. Despite the ancient association with its human host, this epsilon-proteobacterium can cause gastritis, ulcers and gastric cancer. Here we focus on multiple aspects of the genome level...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2007.09.006
更新日期:2007-12-01 00:00:00
abstract::The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90194-7
更新日期:1993-06-01 00:00:00
abstract::Mycotoxins are toxic secondary metabolites produced by filamentous fungi that have been detected in food commodities, including grapes and wine. A survey was conducted to assess mycotoxin-producing fungi in grapes destined for wine production. The mycotoxigenic capacity of the isolates was tested in culture media. Gra...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.12.005
更新日期:2005-05-01 00:00:00
abstract::Several studies indicate Actinobacillus (Haemophilus) actinomycetemcomitans and Fusobacterium nucleatum as etiologic agents of periodontal disease. Immunosuppressive factors produced by microorganisms probably contribute to the initiation and evolution of this disease. This study evaluated the antiproliferative activi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.05.008
更新日期:2004-11-01 00:00:00
abstract::Lipoic acid is a covalently-bound enzyme cofactor required for central metabolism all three domains of life. In the last 20 years the pathway of lipoic acid synthesis and metabolism has been established in Escherichia coli. Expression of the genes of the lipoic acid biosynthesis pathway was believed to be constitutive...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.04.005
更新日期:2014-07-01 00:00:00
abstract::Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)00168-6
更新日期:2000-07-01 00:00:00
abstract::An aerotolerant, psychrotolerant anaerobe, anNAG3, was isolated from an acidic forest floor solution (in situ pH of 4.5). Cells of anNAG3 stained Gram-positive did not form spores, and were not motile. Cells were ovoid, approximately 1 microm long and 0.7 microm wide, mostly in pairs, and contained a multi-layered cel...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.06.006
更新日期:2004-12-01 00:00:00
abstract::Randomly cloned fragments (in a size range 1 to 2.5 kb) of DNA from Bifidobacterium longum ATCC 15707, B. adolescentis CIP 64.59T, B. bifidum CIP 64.65 and B. animalis ATCC 25527 were used as hybridization probes to characterize strains of these species and distinguish them from closely related Bifidobacterium species...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80271-3
更新日期:1995-01-01 00:00:00
abstract::Mollicute evolution has been marked by significant changes in genome structure and use of their genetic information. These include a reduction in their genome G+C content and the use by most mollicutes of the UGA universal stop codon as tryptophan. More striking is the size reduction in their genome which, for some sp...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.09.007
更新日期:2007-12-01 00:00:00
abstract::The adsorption complex of filamentous phage fd consists of two minor coat proteins, g3p and g6p, and is considered to be not only a structural entity, but also a functional unit to terminate phage assembly. Cells were infected with phage M13am8H1, which cannot assemble because it lacks the major coat protein g8p, alth...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90042-6
更新日期:1994-11-01 00:00:00
abstract::The structure of spiroplasmas in the Chinese mitten crab associated with tremor disease was studied by transmission electron microscopy techniques, including both negative staining and ultrasectioning. A spiral structure, which is a typical form of spiroplasmas, could be detected and the observations showed for the fi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.04.010
更新日期:2004-10-01 00:00:00
abstract::The information collected in national and international libraries on nucleotide and protein sequences cannot be directly treated for proper handling by existing software. Therefore we evaluated the feasibility of constructing a data base for Escherichia coli using the data present in the banks. The knowhow thus acquir...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(91)90073-j
更新日期:1991-09-01 00:00:00
abstract::A new actinomycete strain designated US24 producing antibacterial activities against Gram-positive and Gram-negative bacteria was isolated from Tunisian soil. Culture characteristic studies strongly suggested that the US24 strain belonged to the genus Streptomyces. Analysis of the nucleotide sequence of the 16S rRNA g...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00077-9
更新日期:2003-06-01 00:00:00
abstract::Haemophilus parainfluenzae and Haemophilus influenzae, upper respiratory tract microbiota representatives, are able to colonize natural and artificial surfaces as biofilm. The aim of the present study was to assay the effect of ten 1,2,4-triazole-ciprofloxacin hybrids on planktonic or biofilm-forming haemophili cells ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.009
更新日期:2016-10-01 00:00:00
abstract::A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90046-6
更新日期:1989-02-01 00:00:00
abstract::The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.014
更新日期:2007-04-01 00:00:00
abstract::The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)01151-7
更新日期:2000-12-01 00:00:00
abstract::Infections caused by Staphylococcus aureus are prevalent. The dramatically reduced discovery of new antibiotics, as well as the persistent emergence of resistant bacteria, represents a major health problem in both hospital and community settings. Using antibiotic enhancers to rescue existing classes of antibiotics is ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.08.002
更新日期:2017-01-01 00:00:00
abstract::Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug developm...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/S0923-2508(02)00013-X
更新日期:2003-03-01 00:00:00
abstract::Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)84020-4
更新日期:1996-10-01 00:00:00
abstract::Development of effective strategies for treatment of candidiasis and other fungal diseases has posed a challenge, considering the increase in opportunistic fungal infections in HIV-positive and immunocompromised patients. The in vitro antifungal activity of essential oil from Ocimum gratissimum was investigated in ord...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.04.004
更新日期:2004-09-01 00:00:00
abstract::Long polar fimbriae (LPF) are related to type I fimbriae in genetic organization and were first identified in Salmonella enterica serovar Typhimurium. Four lpfA genetic variants designated lpfA(O157/OI-141), lpfA(O157/OI-154), lpfA(O26) and lpfA(O113) have been identified in Shiga toxin-producing Escherichia coli (STE...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.06.009
更新日期:2006-03-01 00:00:00
abstract::The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80012-6
更新日期:1998-10-01 00:00:00
abstract::For a number of years now, we have argued that current models for the control of initiation of DNA synthesis, chromosomal partitioning and septum formation in Escherichia coli are unsatisfactory. Indeed, we could argue that despite considerable efforts, with the possible exception of dnaA and ftsZ, no genes specifical...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90031-5
更新日期:1991-02-01 00:00:00