Abstract:
:Most recently published complete bacterial genomes have revealed unexpectedly high numbers of long strict repeats. In this article we discuss the various functional and evolutionary roles of these repeats, focusing in particular on their role in terms of genome stability, gene transfer, and antigenic variation.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Rocha EP,Danchin A,Viari Adoi
10.1016/s0923-2508(99)00120-5subject
Has Abstractpub_date
1999-11-01 00:00:00pages
725-33issue
9-10eissn
0923-2508issn
1769-7123pii
S0923-2508(99)00120-5journal_volume
150pub_type
杂志文章,评审abstract::At the beginning of the 20th century, it was discovered at the Pasteur Institute in Tunis that epidemic typhus is transmitted by the human body louse. The complete genome sequence of its causative agent, Rickettsia prowazekii, was determined at Uppsala University in Sweden at the end of the century. In this mini-revie...
journal_title:Research in microbiology
pub_type: 传,历史文章,杂志文章,评审
doi:10.1016/s0923-2508(00)00116-9
更新日期:2000-03-01 00:00:00
abstract::Urease biogenesis was monitored in the lactic acid bacterium Streptococcus thermophilus during the growth cycle using in-gel detection and a phenol-hypochloride assay. Zymogram analysis, performed in a non-denaturing polyacrylamide gel, enabled visualization of a complex profile of bands whose number and intensity wer...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.04.005
更新日期:2005-11-01 00:00:00
abstract::A total of 176 strains of Salmonella enterica representing 116 serotypes were tested for the presence of the T6 epitope of the alpha-1,2-linked N-acetyl-D-glucosamine residue by reaction with a murine monoclonal antibody T6 specific for this structure in the Salmonella Ra core lipopolysaccharide (LPS). All 20 serotype...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90185-d
更新日期:1991-06-01 00:00:00
abstract::The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2005.12.002
更新日期:2006-05-01 00:00:00
abstract::For nearly one hundred years, researchers have attempted to categorize botulinum neurotoxin-producing clostridia and the toxins that they produce according to biochemical characterizations, serological comparisons, and genetic analyses. Throughout this period the bacteria and their toxins have defied such attempts at ...
journal_title:Research in microbiology
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.resmic.2014.09.007
更新日期:2015-05-01 00:00:00
abstract::Replicon typing is the identification of plasmids by hybridization with specific DNA probes which contain the genes involved in plasmid maintenance. This new method has been used to classify plasmids into replicon (rep) groups which can often be correlated with incompatibility (Inc) groups. We studied 71 multiresistan...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90063-9
更新日期:1994-01-01 00:00:00
abstract::Mrd1 is one of the trans-acting proteins and plays an important role in precursor ribosomal RNA processing. Here we characterized the Mamrd1 gene from Metarhizium acridum and studied its function in growth, conidiation and virulence using RNA interference. The Mamrd1 gene was identified as participating in the process...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.05.004
更新日期:2011-09-01 00:00:00
abstract::Some pathogenic species belonging to the Vibrionaceae family have been shown to regulate virulence through a complicated network of quorum sensing systems. In this study, three kinds of N-acylhomoserine lactone (AHL) signal molecules were detected in Vibrio ichthyoenteri DA3, a pathogen of cultured turbot (Scophthalmu...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.10.004
更新日期:2010-01-01 00:00:00
abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90191-c
更新日期:1991-06-01 00:00:00
abstract::A method which enables selection of protein mutants impaired in their ability to interact with their normal protein partners is presented here. The method is the two-phage two-hybrid assay adapted for mutant selection. In the two-phage assay, the interaction between two proteins enables the formation of a functional h...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.01.001
更新日期:2010-03-01 00:00:00
abstract::Ribosomal DNA (rDNA) polymorphism was compared to enzyme polymorphism and DNA/DNA hybridization data for the intraspecies differentiation of Providencia alcalifaciens. DNA from 27 strains previously classified into two zymotypes A1 and A2 and discriminated by two levels of DNA/DNA hybridization (delta Tm values of 0 t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90006-v
更新日期:1991-11-01 00:00:00
abstract::A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.07.008
更新日期:2014-09-01 00:00:00
abstract::The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90059-y
更新日期:1990-07-01 00:00:00
abstract::Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90013-2
更新日期:1989-07-01 00:00:00
abstract::A gene encoding a sixth chitin synthase (Umchs6, sequence GenBank accession No. ) from the plant pathogenic hemibasidiomycete Ustilago maydis (DC.) Cda. was isolated and characterized. The predicted protein is 1103 amino acids in length with a calculated molecular mass of 123.5 kDa. a2b2 null mutants were obtained by ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2003.11.005
更新日期:2004-03-01 00:00:00
abstract::Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2015.05.001
更新日期:2015-11-01 00:00:00
abstract::Translation termination in eukaryotes is mediated by two polypeptide chain-release factors, eRF1 and eRF3. eRF1 recognizes stop signals, while eRF3 is a ribosome-dependent and eRF1-dependent GTPase. eRF1 forms a stable complex with eRF3 in vivo and in vitro. In the present study, a variety of truncated forms of Euplot...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.07.005
更新日期:2006-11-01 00:00:00
abstract::Vegetative incompatibility limits heterokaryon formation in fungi. It results from genetic differences at specific loci (het loci). Characterization of het genes and, more recently, of incompatibility reaction suppressors has, provided insight into the mechanisms involved. A link between development, vegetative incomp...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(00)00145-5
更新日期:2000-05-01 00:00:00
abstract::Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2003.11.001
更新日期:2004-03-01 00:00:00
abstract::This paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of L. monocyto...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.03.004
更新日期:2019-01-01 00:00:00
abstract::A new type of nitrogen-fixing, oxalate-oxidizing Azospirillum sp. was isolated from the roots of Oxalis pes-caprae. Polyphasic taxonomy was performed, including auxanography using API galleries, physiological tests and 16S rRNA sequence comparison. Optimum growth occurred at 30 degrees C, pH 7.5. Growth was observed a...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2004.10.009
更新日期:2005-04-01 00:00:00
abstract::In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90016-j
更新日期:1990-06-01 00:00:00
abstract::Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.06.010
更新日期:2016-10-01 00:00:00
abstract::Amplified fragment length polymorphism (AFLP), a novel method for molecular typing, was evaluated for its ability to differentiate among a group of highly related Chlamydia psittaci strains isolated from ruminants and belonging to serotype 1. A total set of 12 strains were included in this study, 10 strains inducing a...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80020-5
更新日期:1998-11-01 00:00:00
abstract::A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90046-6
更新日期:1989-02-01 00:00:00
abstract::Pseudomonas mendocina carrying a novel class 1 integron containing an IMP-8 gene was isolated from an inanimate surface in a female ward sanitary facility of the Hospital Infante D. Pedro, Aveiro, Portugal. Hybridization with the integrase gene (intI1) and 16S rDNA revealed that the integron is chromosomally located. ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.03.004
更新日期:2010-05-01 00:00:00
abstract::The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.06.005
更新日期:2016-09-01 00:00:00
abstract::Bacteria of the Bacillus cereus group are environmental Gram-positive spore-forming bacteria ubiquitously distributed. Despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychro...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2016.07.002
更新日期:2017-05-01 00:00:00
abstract::A common effluent treatment plant (CETP) is a biological wastewater treatment facility that receives wastewater from different industries. The activated biomass in the CETP survives on a wide range of chemicals with no fixed wastewater characteristics. We carried out a diversity analysis of this activated biomass usin...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.04.004
更新日期:2007-07-01 00:00:00
abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90193-9
更新日期:1989-11-01 00:00:00