Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns.

Abstract:

:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only given by biotype cholerae (14 strains studied). Thirteen patterns (B5 to B17) were only given by biotype El Tor (75 strains studied). There was no correlation between serotypes and rRNA gene restriction patterns. This study provides arguments that (1) strains of biotypes cholerae and El Tor are different clones, (2) a cholera pandemic is not a single world-wide epidemic (due to a single clone) but rather a simultaneous occurrence of several epidemics (several clones involved), and (3) epidemic waves of biotype El Tor could be due to the emergence of new clones.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Koblavi S,Grimont F,Grimont PA

doi

10.1016/0923-2508(90)90059-y

subject

Has Abstract

pub_date

1990-07-01 00:00:00

pages

645-57

issue

6

eissn

0923-2508

issn

1769-7123

pii

0923-2508(90)90059-Y

journal_volume

141

pub_type

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