Abstract:
:The rRNA gene restriction patterns of 89 Vibrio cholerae O1 isolates from different geographic origins were studied. The probe was Escherichia coli 16 + 23S rRNA labelled with "ECL Gene detection system". A total of 17 rRNA gene restriction patterns were observed after BglI cleavage. Four patterns (B1 to B4) were only given by biotype cholerae (14 strains studied). Thirteen patterns (B5 to B17) were only given by biotype El Tor (75 strains studied). There was no correlation between serotypes and rRNA gene restriction patterns. This study provides arguments that (1) strains of biotypes cholerae and El Tor are different clones, (2) a cholera pandemic is not a single world-wide epidemic (due to a single clone) but rather a simultaneous occurrence of several epidemics (several clones involved), and (3) epidemic waves of biotype El Tor could be due to the emergence of new clones.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Koblavi S,Grimont F,Grimont PAdoi
10.1016/0923-2508(90)90059-ysubject
Has Abstractpub_date
1990-07-01 00:00:00pages
645-57issue
6eissn
0923-2508issn
1769-7123pii
0923-2508(90)90059-Yjournal_volume
141pub_type
杂志文章abstract::The development of nascent cell division sites was studied in Escherichia coli strains containing ftsAts and ftsZts mutations in which septal development is arrested after shift to the restrictive temperature. Division sites were studied by measuring positions of plasmolysis bays, a visible marker for periseptal annul...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90047-e
更新日期:1991-02-01 00:00:00
abstract::Sub-MIC antibiotics differentially modulate transcription of subsets of genes by unknown mechanisms. Paradoxically, the RNA polymerase inhibitor rifampicin is able to both upmodulate as well as downmodulate transcription when present at sub-MIC levels. In this study, we analyzed DNA sequences required for transcriptio...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.02.003
更新日期:2013-06-01 00:00:00
abstract::To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2017.11.005
更新日期:2018-01-01 00:00:00
abstract::Production of aerobactin has been reported to be a virulence factor in members of the family Enterobacteriaceae. To investigate the protection afforded by humoral immunity directed towards aerobactin in infectious diseases caused by aerobactin-producing strains, we tested the efficacy of mAbAERO1, a murine monoclonal ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80894-1
更新日期:1995-02-01 00:00:00
abstract::The results of a multiple locus variable number of tandem repeat (VNTR) analysis (MLVA)-based study designed to understand the genetic diversity of soil and manure-borne Pseudomonas aeruginosa isolates, and the relationship between these isolates and a set of clinical and environmental isolates, are hereby reported. F...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.10.004
更新日期:2014-01-01 00:00:00
abstract::5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90048-7
更新日期:1993-03-01 00:00:00
abstract::Bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. In this study, the prevalence of B. cereus bacteriophages in Korean fermented foods was studied using 6 reference strains, and JBP901 was further characterized. In a total of 47 samples, ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.07.001
更新日期:2011-10-01 00:00:00
abstract::This project focused on the effects of aflatoxin B1 (AFB1), a food-contaminating mycotoxin produced by fungi, genus Aspergillus, on the release and genetic expression of some important cytokines, i.e., (interleukin-1 alpha (IL-1 alpha), IL-6, tumor necrosis factor-alpha (TNF alpha)) by human monocytes. Monocytes, prei...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80042-4
更新日期:1999-01-01 00:00:00
abstract::A Gram-negative, short rod, aerobic bacterium, designated W11(T), was isolated from seawater. Heterotrophic growth was observed at 10-45 °C and pH 6-10. Optimal growth was observed at 30-37 °C and pH 7-9. It can grow in the presence of 0.5-12% NaCl (w/v), and the optimal NaCl required for growth was 5-6%. 16S rRNA gen...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.07.008
更新日期:2014-09-01 00:00:00
abstract::Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(03)00113-X
更新日期:2003-07-01 00:00:00
abstract::Microbial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one - the arsenivorans strain of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2019.09.006
更新日期:2020-01-01 00:00:00
abstract::Eighty-one strains of Yersinia enterocolitica biovar 1A representing several serotypes isolated from India, France, Germany and the USA were analyzed using ribotyping, 16S-23S rDNA intergenic spacer length polymorphism analysis (PCR-ribotyping) and gyrB restriction fragment length polymorphism. Ribotyping with BglI, N...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.11.011
更新日期:2007-04-01 00:00:00
abstract::RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2020.06.001
更新日期:2020-01-01 00:00:00
abstract::The present mini-review summarizes data in selected fields of basic genetics which were exclusively obtained in agreement with the principles of SOS Chromotest methodology and with Escherichia coli PQ37 sfiA::lacZ as a tester strain. ...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(02)01342-6
更新日期:2002-09-01 00:00:00
abstract::The work described here continues our studies of the effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus. Mitomycin C and the new-generation fluoroquinolone moxifloxacin induced expression of SOS response genes (lexA, recA, sosA, and umuC) in a ciproflo...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.09.013
更新日期:2009-12-01 00:00:00
abstract::rRNA gene restriction patterns (ribotyping) were compared with phage typing, serotyping, enterotoxins and exfoliatin production in the analysis of 26 Staphylococcus aureus strains isolated from two different nosocomial outbreaks. Total DNA was cleaved by EcoRI restriction endonuclease. After agarose gel electrophoresi...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90212-k
更新日期:1993-01-01 00:00:00
abstract::The aim of the present work was to develop polymerase chain reactions (PCRs) based on the conserved nucleotide sequence of the 16S rRNA gene for detection of bacteria of the Helicobacter genus in human antral biopsy samples. The assay for Helicobacter spp was developed by amplifying a 399-bp 16S rRNA gene sequence spe...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/S0923-2508(97)81587-2
更新日期:1997-05-01 00:00:00
abstract::Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.06.002
更新日期:2014-09-01 00:00:00
abstract::The neurotoxin gene from a strain of Clostridium botulinum type A causing infant botulism was cloned as a series of overlapping polymerase chain reaction (PCR) fragments generated using primers designed to conserved regions of published botulinal toxin (BoNT) sequences. Translation of the nucleotide sequence derived f...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90004-l
更新日期:1993-09-01 00:00:00
abstract::Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related p...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.01.008
更新日期:2013-05-01 00:00:00
abstract::In Neisseria gonorrhoeae, all penicillinase-specifying plasmids isolated so far share homology with each other and carry a 60% deleted sequence of TnA. Plasmid pHD131, an element isolated from Haemophilus ducreyi and carrying an intact copy of the ampicillin resistance transposable element, was introduced from Escheri...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90016-j
更新日期:1990-06-01 00:00:00
abstract::When grown in batch cultures in fermentors with 23.4 mM cellobiose, Clostridium cellulolyticum displayed biphasic growth kinetics not associated with sequential substrate consumption and which led to a twofold higher production of biomass than previously reported. In the first growth phase, acetate was the major produ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)00110-2
更新日期:1999-09-01 00:00:00
abstract::Although great strides have been made in the development of recombinant attenuated Vibrio cholerae vaccine strains, the task has not been as simple as once imagined. The initial vaccine candidates proved to be unexpectedly reactogenic but further derivatives, such as CVD103-HgR, are well-tolerated, immunogenic and pro...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(90)90128-d
更新日期:1990-09-01 00:00:00
abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80037-0
更新日期:1999-04-01 00:00:00
abstract::The diversity and temporal dynamics of bacterial communities in pristine snow around two Russian Antarctic stations was investigated. Taxonomic analysis of rDNA libraries revealed that snow communities were dominated by bacteria from a small number of operational taxonomic units (OTUs) that underwent dramatic swings i...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.08.005
更新日期:2013-11-01 00:00:00
abstract::Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01373-6
更新日期:2002-11-01 00:00:00
abstract::The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphot...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)01151-7
更新日期:2000-12-01 00:00:00
abstract::In this study, we identified an antimicrobial compound produced by the Gram-negative bacterium Serratia marcescens. Colonies of S. marcescens inhibited the growth of nine different methicillin-resistant Staphylococcus aureus (MRSA) isolates and several other tested Gram-positive bacterial species, but not Gram-negativ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2013.06.002
更新日期:2013-10-01 00:00:00
abstract::Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.02.003
更新日期:2012-05-01 00:00:00
abstract::Jarosite precipitates formed in iron oxidising bioreactors have been shown to harbour iron-oxidisers. The aim of this study was to develop an iron oxidising bioprocess where microorganisms are retained solely on biogenic jarosite particles. Based on preliminary experiments using a fluidised-bed bioreactor (FBR), the f...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2020.06.004
更新日期:2020-01-01 00:00:00