Abstract：
:Sequences of the most conserved proteins encoded by transposable (pro)phages were used to search recently sequenced Firmicute genomes for candidate transposable prophages. One complete Mu-like prophage, SglyMu-1, was identified, in four copies, in the Syntrophobotulus glycolicus DSM 8271 chromosome sequence. Related prophages were also found in partially assembled genomic sequences of other Firmicutes and newly sequenced Proteobacteria genomes, opening the road to the use of Mu-like derived genetic tools in Gram(+) bacteria. SglyMu-1 appears to carry a host variation system related to the DGR tropism switching retroelements, first characterized in Bordetella phages BPP-1, BIP-1 and BMP-1. Transposable phages are thus thriving among Firmicutes and can harness either of two host variation systems, the fiber genes inversion and reverse transcriptase-mediated site-directed, adenine-specific mutagenesis.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Toussaint Adoi
10.1016/j.resmic.2013.01.008subject
Has Abstractpub_date
2013-05-01 00:00:00pages
281-7issue
4eissn
0923-2508issn
1769-7123pii
S0923-2508(13)00009-0journal_volume
164pub_type
杂志文章abstract：:Soil is one of the most complex and challenging environments for microbiologists. In fact, although it contains the largest microbial diversity on the planet, the majority of these microbes are still uncharacterized and represent an enormous unexplored reservoir of genetic and metabolic diversity. Metagenomics, the st...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
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journal_title：Research in microbiology
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pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
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abstract：:Viruses play important roles in aquatic ecosystems, but deep-sea bacteriophages remain largely unexplored. A temperate bacteriophage (termed vB_PstS-1) was identified from the psychrotolerant gammaproteobacterium Pseudomonas stutzeri 1-1-1b, which was isolated from hadopelagic water (depth of 7000 m) of the Japan Tren...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2015.05.001
更新日期：2015-11-01 00:00:00
abstract：:Recent studies suggest that viruses are the most numerous entities in the biosphere; bacteriophages, the viruses that infect Eubacteria and Archaea, constitute a substantial fraction of this population. In spite of their ubiquity, the vast majority of phages in the environment have never been studied and nothing is kn...
journal_title：Research in microbiology
pub_type： 杂志文章
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
abstract：:We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains Al12(T) and Al16 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg o...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.06.004
更新日期：2006-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.07.005
更新日期：2013-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01378-5
更新日期：2002-12-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2005.01.014
更新日期：2005-06-01 00:00:00
abstract：:DNA-DNA hybridization showed that the Brucella strains recently isolated from marine mammals belong to the monospecific genus Brucella (more than 77% DNA relatedness). Ribotyping (HindIII rDNA restriction patterns) showed that they may represent a separate subgroup (marine type) specifically associated with marine mam...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01145-1
更新日期：2000-11-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.02.003
更新日期：2008-05-01 00:00:00
abstract：:Both Neisseria meningitidis and Streptococcus pneumoniae are naturally transformable species and are known to be freely recombining in the wild. Large multilocus sequence typing (MLST) datasets have been generated for these species. Here we outline an approach which exploits these data sets in order to quantify the ex...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(00)00168-6
更新日期：2000-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.08.002
更新日期：2003-11-01 00:00:00
abstract：:In order to simplify diagnostic susceptibility testing of mycobacteria against thiacetazone, hydroxylamine, p-nitrobenzoic acid and picric acid, we modified the standard procedure by using Middlebrook 7H11 agar in a single quadrant petri dish. This plate can be inoculated and read in parallel with routine susceptibili...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90078-8
更新日期：1989-03-01 00:00:00
abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2017.11.005
更新日期：2018-01-01 00:00:00
abstract：:The mechanism by which TrwB acts as a DNA transporter in bacterial conjugation is analyzed. Based on a parallelism between TrwB and F(1)-ATPase, TrwB would use the energy derived from ATP hydrolysis to pump DNA through its central channel, in a manner similar to that used by F(1)-ATPase to produce a rotary movement of...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/j.resmic.2005.12.002
更新日期：2006-05-01 00:00:00
abstract：:Association with a surface in a structure known as biofilm is the prevailing microbial lifestyle. Here we show the kinetics of biofilm formation of Escherichia coli W3110 in static cultures growing under aerobic or anaerobic conditions. Aerobically growing cells in LB medium started to produce detectable amounts of bi...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.03.004
更新日期：2004-09-01 00:00:00
abstract：:The tricarballylate utilization locus (tcuRABC) of Salmonella enterica serovar Typhimurium is comprised of a 3-gene operon (tcuABC) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcuR gene, which encodes a putative LysR-type transcriptional regulator. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.01.001
更新日期：2009-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01151-7
更新日期：2000-12-01 00:00:00
abstract：:The expression of the feruloyl esterase gene faeA, the alpha-glucuronidase gene aguA, the endoxylanase gene xlnB, and the beta-xylosidase gene xlnD from Aspergillus niger on xylose was studied in a wild-type strain and in a CreA mutant. A decrease in expression of all four genes was observed with increasing xylose con...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80053-9
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2017.09.005
更新日期：2018-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.05.008
更新日期：2004-11-01 00:00:00
abstract：:Evidence of increasing resistance to antibiotics in soil and other natural isolates highlights the importance of horizontal transfer of resistance genes in facilitating gene flux in bacteria. Horizontal gene transfer in bacteria is favored by the presence of mobile genetic elements and by the organization of bacterial...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01215-3
更新日期：2001-06-01 00:00:00
abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/S0923-2508(03)00113-X
更新日期：2003-07-01 00:00:00
abstract：:In the genus Pseudomonas, two main lineages and 19 phylogenetic groups or subgroups have been defined based on DNA sequence studies of 16S rRNA, gyrB and rpoD genes. In the present study, 33 strains previously classified as Pseudomonas putida were phylogenetically affiliated with their closest relatives in the genus b...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2013.01.009
更新日期：2013-05-01 00:00:00
abstract：:The understanding of biofilm formation by bioleaching microorganisms is of great importance for influencing mineral dissolution rates and to prevent acid mine drainage (AMD). Thermo-acidophilic archaea such as Acidianus, Sulfolobus and Metallosphaera are of special interest due to their ability to perform leaching at ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.06.005
更新日期：2016-09-01 00:00:00
abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(92)90031-i
更新日期：1992-01-01 00:00:00
abstract：:Eight strains of acidophilic bacteria, isolated from mine-impacted and geothermal sites from different parts of the world, were shown to form a distinct clade (proposed genus "Acidibacillus") within the phylum Firmicutes, well separated from the acidophilic genera Sulfobacillus and Alicyclobacillus. Two of the strains...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2016.04.008
更新日期：2016-09-01 00:00:00
