Abstract：
:Campylobacter spp. is the most common cause of bacterial diarrhoea in humans worldwide. Therefore, rapid and reliable methods for detection and quantification of this pathogen are required. In this study, we have developed a reverse transcription quantitative real-time PCR (RT-qPCR) for detection and quantification of viable Campylobacter jejuni directly from chicken faecal samples. The results of this method and a DNA-based quantitative real-time PCR (qPCR) method were compared with those of a bacterial culture method. Using bacterial culture and RT-qPCR methods, viable C. jejuni cells could be detected for up to 5 days in both the C. jejuni spiked and the naturally contaminated faecal samples. We found that no RT-qPCR signals were obtained when viable C. jejuni cells could not be counted by the culture method. In contrast, using a DNA-based qPCR method, dead or non-viable Campylobacter cells were detected, and all tested samples were positive, even after 20 days of storage. The developed method for detection and quantification of viable C. jejuni cells directly from chicken faecal samples can be used for further research on the survival of Campylobacter in the environment.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Bui XT,Wolff A,Madsen M,Bang DDdoi
10.1016/j.resmic.2011.10.007subject
Has Abstractpub_date
2012-01-01 00:00:00pages
64-72issue
1eissn
0923-2508issn
1769-7123pii
S0923-2508(11)00173-2journal_volume
163pub_type
杂志文章abstract：:A bacteriolytic enzyme excreted by Pseudomonas aeruginosa Paks I was purified: samples were found to be homogeneous by gel filtration chromatography, ion exchange chromatography using CM-cellulose, immunoelectrophoresis, PAGE and SDS-PAGE. The molecular weight of the lytic enzyme was estimated to be 15,000-19,000. The...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/0923-2508(89)90046-6
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abstract：:Magnetotactic bacteria (MTB) are a group of Gram-negative bacteria synthesizing magnetic crystals that allow them aligning along magnetic field lines. They have diverse morphologies including cocci, rods, vibrio, spirilla, and multicellular magnetotactic prokaryotes (MMPs). MMPs are composed of 10-57 cells with peritr...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.012
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pub_type： 杂志文章
doi：10.1016/s0923-2508(02)01332-3
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pub_type： 杂志文章
doi：10.1016/0923-2508(94)90062-0
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.08.003
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2007.04.004
更新日期：2007-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.09.005
更新日期：2005-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0923-2508(92)90075-y
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pub_type： 杂志文章
doi：10.1016/s0923-2508(00)01172-4
更新日期：2001-01-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.03.003
更新日期：2009-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 临床试验,杂志文章,评审
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pub_type： 历史文章,杂志文章,评审
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更新日期：2015-05-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(01)01194-9
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01342-6
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pub_type： 杂志文章
doi：10.1016/0923-2508(96)81064-3
更新日期：1995-10-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/0923-2508(96)80289-0
更新日期：1995-07-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2003.11.003
更新日期：2004-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2008.10.012
更新日期：2009-03-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/s0923-2508(99)80042-4
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journal_title：Research in microbiology
pub_type： 杂志文章,评审
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2006.11.011
更新日期：2007-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2004.06.006
更新日期：2004-12-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
abstract：:The tricarballylate utilization locus (tcuRABC) of Salmonella enterica serovar Typhimurium is comprised of a 3-gene operon (tcuABC) that encodes functions that allow this bacterium to use tricarballylate as a source of carbon and energy, and the tcuR gene, which encodes a putative LysR-type transcriptional regulator. ...
journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2009.01.001
更新日期：2009-04-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:The naturally transformable bacterium Haemophilus influenzae Rd contains 1471 copies of the DNA uptake signal sequence (USS) 5'-AAGTGCGGT in its genome. Neisseria meningitidis contains 1891 copies of the USS sequence 5'-GCCGTCTGAA. The USSs are often found in the base paired stem of transcription terminators. ...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(99)00130-8
更新日期：1999-11-01 00:00:00
abstract：:Type IV secretory systems are transmembrane bacterial multiprotein complexes. They are pivotal for conjugation, bacterial-induced plant tumour formation, toxin secretion and mammalian pathogen intracellular activity. These systems are involved in the spread of antibiotic resistance genes among bacteria by enabling con...
journal_title：Research in microbiology
pub_type： 杂志文章,评审
doi：10.1016/s0923-2508(02)01313-x
更新日期：2002-05-01 00:00:00
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journal_title：Research in microbiology
pub_type： 杂志文章
doi：10.1016/j.resmic.2014.07.005
更新日期：2014-11-01 00:00:00
abstract：:While the scientific contributions of François Jacob were outstanding, I also consider that his conception of science, and of its place among other forms of knowledge, is also highly original, and important for the future of science in our societies. His contributions to the history and philosophy of science were neit...
journal_title：Research in microbiology
pub_type： 传,历史文章,杂志文章
doi：10.1016/j.resmic.2014.05.001
更新日期：2014-06-01 00:00:00
