Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium.

abstract：:The role of protein-tyrosine kinases in bacterial polymyxin resistance was assessed by both genetic and biochemical approaches. Each of the two genes, wzc and etk, encoding protein-tyrosine kinases in Escherichia coli, was knocked out by using the PCR-based method of one-step inactivation of chromosomal genes, and the...

journal_title：Research in microbiology

authors： Lacour S,Doublet P,Obadia B,Cozzone AJ,Grangeasse C

更新日期：2006-09-01 00:00:00

abstract：:In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...

journal_title：Research in microbiology

authors： Levine MM,Hone D,Tacket C,Ferreccio C,Cryz S

更新日期：1990-09-01 00:00:00

abstract：:Cell-free extracts of various strains belonging to Mycobacterium paratuberculosis (Ptb) and "wood-pigeon" (WP) mycobacteria were compared by polyacrylamide gel electrophoresis and the various protein bands obtained were tested for peroxidase enzyme activity. One strain of Mycobacterium avium served as a control. Bacte...

journal_title：Research in microbiology

authors： Thorel MF,Blom-Potar MC,Rastogi N

更新日期：1990-06-01 00:00:00

abstract：:Sequences of the lacSZ operon of 29 Streptococcus thermophilus strains from different dairy products were determined. Differences in sequence among the strains were detected within LacS more often than in the LacZ gene. The sequences were aligned and compared and it was possible to gather the strains into three groups...

journal_title：Research in microbiology

authors： Ercolini D,Fusco V,Blaiotta G,Coppola S

更新日期：2005-03-01 00:00:00

abstract：:Deinococcus radiodurans is a bacterium best known for its extreme resistance to high levels of ionizing radiation. Gene expression profiles of D. radiodurans exposed to 0.3 M NaCl revealed that at least 389 genes were induced and 415 were repressed by twofold or more. A general down-regulation of the central metabolic...

journal_title：Research in microbiology

authors： Im S,Joe M,Kim D,Park DH,Lim S

更新日期：2013-11-01 00:00:00

abstract：:Sporulation histidine kinases, which sense sporulation-specific signals and initiate phosphorelay reactions, are poorly conserved among Bacillus species. We found several putative genes for sporulation histidine kinases in the genome sequence of Paenibacillus polymyxa E681 and assayed the genes for complementation of ...

journal_title：Research in microbiology

authors： Park SY,Park SH,Choi SK

更新日期：2012-05-01 00:00:00

abstract：:The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littor...

journal_title：Research in microbiology

authors： Meunier JR,Grimont PA

更新日期：1993-06-01 00:00:00

abstract：:Rhizobium leguminosarum bv. trifolii synthesizes extracellular polysaccharide (EPS) that is postulated to be a biologically active signalling molecule in clover symbiosis. A group of seven exopolysaccharide-deficient (Exo), non-nitrogen-fixing mutants of R. leguminosarum bv. trifolii strain 24.1 isolated by transposon...

journal_title：Research in microbiology

authors： Janczarek M,Skorupska A

更新日期：2003-07-01 00:00:00

abstract：:A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic stream draining an abandoned copper mine in north Wales. The isolate (PY-F3) was demonstrated to be a heterotroph that catalyzed the oxidation of ferrous iron (but not of sulfur or hydrogen) under aerobic conditions, and the reduction ...

journal_title：Research in microbiology

authors： Jones RM,Johnson DB

更新日期：2015-02-01 00:00:00

abstract：:The major 25-kDa outer membrane protein (Omp25) of Brucella abortus was purified and antigenically characterized by use of monoclonal antibodies (mAbs). Purification was achieved from the sodium dodecyl sulphate-insoluble (SDS-I) cell wall (CW) fraction of vaccine strain B. abortus B19 which was shown by use of mAbs t...

journal_title：Research in microbiology

authors： Cloeckaert A,Zygmunt MS,Bézard G,Dubray G

更新日期：1996-05-01 00:00:00

abstract：:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug developm...

journal_title：Research in microbiology

authors： Schumacher MA,Brennan RG

更新日期：2003-03-01 00:00:00

abstract：:The marine psychrophilic bacterium Moritella viscosa is the causative agent of winter ulcer in farmed Atlantic salmon and cod. In this study, the growth requirements of the pathogen were established. The effects of changes in salinity and temperature on growth, surface features and proteomic regulation were also inves...

journal_title：Research in microbiology

authors： Tunsjø HS,Paulsen SM,Mikkelsen H,L'abée-Lund TM,Skjerve E,Sørum H

更新日期：2007-04-01 00:00:00

abstract：:Plasmids that express an enzymatically active beta-galactosidase in an Escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing Vibrio cholerae chromosomal DNA. Deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb DNA fragment, and the ge...

journal_title：Research in microbiology

authors： Parsot C

更新日期：1992-01-01 00:00:00

abstract：:WhiB is an essential sporulation factor in Streptomyces coelicolor. We report here the molecular genetic characterisation of whiB3, a whiB-like gene in the nonspore-forming Mycobacterium smegmatis mc(2)155. M. smegmatis whiB3 encodes a 96-amino-acid protein with 81% similarity to its M. tuberculosis counterpart identi...

journal_title：Research in microbiology

authors： Hutter B,Dick T

更新日期：1999-06-01 00:00:00

abstract：:Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...

journal_title：Research in microbiology

authors： Ashida H,Danchin A,Yokota A

更新日期：2005-06-01 00:00:00

abstract：:Heterotrophic microaerophilic bacteria, a diverse and ubiquitous group, are specialized for growth in O2 limited environments. Until recently, microaerophilic bacteria have been grouped with obligate aerobic organisms whose metabolic rates slow in response to physiological O2 limitation. In contrast, microaerophilic b...

journal_title：Research in microbiology

authors： Ludwig RA

更新日期：2004-03-01 00:00:00

abstract：:A collection of 57 strains of hyperthermophilic Archaea from the order Thermococcales was screened for the presence of plasmids; 9 plasmids present in six of these strains were isolated and characterized in terms of size and cross-hybridization. The Notl macrorestriction patterns of genomic DNA of strains harbouring t...

journal_title：Research in microbiology

authors： Benbouzid-Rollet N,López-García P,Watrin L,Erauso G,Prieur D,Forterre P

更新日期：1997-12-01 00:00:00

abstract：:Growth of mesophilic Aeromonas sp. strains from serogroups O:13, O:33 and O:44 at different temperatures and osmolarity resulted in changes in the lipopolysaccharide (LPS) and virulence of the strains tested, as we had previously reported for strains from serogroup O:34. The effect of osmolarity could be observed when...

journal_title：Research in microbiology

authors： Merino S,Aguilar A,Rubires X,Tomás JM

更新日期：1998-06-01 00:00:00

abstract：:The Escherichia coli mannitol permease (EIIMtl) of the phosphoenolpyruvate-dependent phosphotransferase system is a 68-kDa membrane protein that carries out the concomitant transport and phosphorylation of D-mannitol. Previous studies indicated that there are ca. 6 membrane-spanning helices within the N-terminal half ...

journal_title：Research in microbiology

authors： Briggs CE,Khandekar SS,Jacobson GR

更新日期：1992-02-01 00:00:00

abstract：:The development of nascent cell division sites was studied in Escherichia coli strains containing ftsAts and ftsZts mutations in which septal development is arrested after shift to the restrictive temperature. Division sites were studied by measuring positions of plasmolysis bays, a visible marker for periseptal annul...

journal_title：Research in microbiology

authors： Cook WR,Rothfield LI

更新日期：1991-02-01 00:00:00

abstract：:The insertion of proteins into membranes generally requires the assistance of membrane proteins. A protein, designated Omp85 in Neisseria meningitidis, was shown to be required for the assembly of bacterial outer-membrane proteins. The protein is essential for the viability of the bacteria and is ubiquitous among Gram...

journal_title：Research in microbiology

authors： Voulhoux R,Tommassen J

更新日期：2004-04-01 00:00:00

abstract：:Paralytic shellfish toxins (PSTs) are sodium channel blocking (SCB) toxins, produced by cyanobacteria, as well as by marine dinoflagellates and their associated bacteria, and cause serious health and economic concern worldwide. In a previous study, approximately 70% of the bacteria enriched from PST-contaminated shell...

journal_title：Research in microbiology

authors： Vásquez M,Grüttner C,Möeller B,Moore ER

更新日期：2002-07-01 00:00:00

abstract：:To understand antibiotic resistance in Gram-negative bacteria, a key point is to investigate antibiotic accumulation, which is defined by influx and efflux. Several methods exist to evaluate membrane permeability and efflux pump activity, but they present disadvantages and limitations. An optimized spectrofluorimetric...

journal_title：Research in microbiology

authors： Masi M,Dumont E,Vergalli J,Pajovic J,Réfrégiers M,Pagès JM

更新日期：2018-01-01 00:00:00

abstract：:The Pseudomonas aeruginosa protein AlgR2 (AlgQ) was originally identified as a regulatory protein implicated in alginate production. It also regulates the synthesis of polyphosphate as well as of a variety of secretable virulence factors, upregulating neuraminidase and siderophore synthesis and downregulating rhamnoli...

journal_title：Research in microbiology

authors： Ledgham F,Soscia C,Chakrabarty A,Lazdunski A,Foglino M

更新日期：2003-04-01 00:00:00

abstract：:Members of the genus Cronobacter are responsible for cases of meningitis and bacteremia with high fatality rates in neonates. Macrophage uptake of invading microbes is an innate process, and it has been proposed that macrophage infectivity potentiator (Mip) like proteins enhance the ability of pathogens to survive wit...

journal_title：Research in microbiology

authors： Eshwar AK,Tasara T,Stephan R,Lehner A

更新日期：2015-04-01 00:00:00

abstract：:Explosives such as octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) provide a challenge in terms of bioremediation. In the present study, sheep rumen was studied for its potential to detoxify HMX using analytical chemistry and molecular microbial ecology tools. Results indicated significant loss (p < 0.05) of HM...

journal_title：Research in microbiology

authors： Perumbakkam S,Craig AM

更新日期：2012-09-01 00:00:00

abstract：:Measuring gene expression at the single cell and single molecule level has recently made possible the quantitative measurement of stochasticity of gene expression. This enables identification of the probable sources and roles of noise. Gene expression noise can result in bacterial population heterogeneity, offering sp...

journal_title：Research in microbiology

authors： Bury-Moné S,Sclavi B

更新日期：2017-01-01 00:00:00

abstract：:Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...

journal_title：Research in microbiology

authors： Bassi D,Cappa F,Cocconcelli PS

更新日期：2009-06-01 00:00:00

abstract：:Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...

journal_title：Research in microbiology

authors： Rotem O,Biran D,Ron EZ

更新日期：2013-01-01 00:00:00

abstract：:Ninety-nine strains of Gram-negative black-pigmented anaerobic rods, grown on Todd-Hewitt blood agar plates, were identified and characterized according to a typing scheme including UV fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the ATB 32A kit, and gas-liquid chromato...

journal_title：Research in microbiology

authors： Fournier D,Mouton C

更新日期：1993-07-01 00:00:00