Abstract:
:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug development efforts. The Staphylococcus aureus multidrug binding regulator, QacR, is soluble and recognizes an especially wide range of structurally dissimilar compounds, properties making it an ideal model system for deciphering the molecular basis of multidrug recognition. Recent structures of QacR have afforded the first view of any MDR protein bound to multiple drugs, revealing key structural features of multidrug recognition, including a multisite binding pocket.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Schumacher MA,Brennan RGdoi
10.1016/S0923-2508(02)00013-Xsubject
Has Abstractpub_date
2003-03-01 00:00:00pages
69-77issue
2eissn
0923-2508issn
1769-7123pii
S0923-2508(02)00013-Xjournal_volume
154pub_type
杂志文章,评审abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2007.11.020
更新日期:2008-03-01 00:00:00
abstract::As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.11.002
更新日期:2009-03-01 00:00:00
abstract::Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.03.001
更新日期:2005-06-01 00:00:00
abstract::Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90013-2
更新日期:1989-07-01 00:00:00
abstract::Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90078-7
更新日期:1994-10-01 00:00:00
abstract::Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.07.005
更新日期:2008-11-01 00:00:00
abstract::We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains Al12(T) and Al16 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg o...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2006.06.004
更新日期:2006-11-01 00:00:00
abstract::Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.004
更新日期:2016-10-01 00:00:00
abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90191-c
更新日期:1991-06-01 00:00:00
abstract::Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(02)01373-6
更新日期:2002-11-01 00:00:00
abstract::The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)80012-6
更新日期:1998-10-01 00:00:00
abstract::The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosu...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(01)01188-3
更新日期:2001-03-01 00:00:00
abstract::In heap bioleaching and waste-rock dumps, complex microbial communities exist in the flowing and interstitial liquid phases and mineral surface-associated biofilms, often embedded in extracellular polymeric substances (EPS). Microbial activity in the interstitial phase and mineral ore surface facilitates mineral degra...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2020.09.002
更新日期:2020-01-01 00:00:00
abstract::5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90048-7
更新日期:1993-03-01 00:00:00
abstract::In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...
journal_title:Research in microbiology
pub_type: 临床试验,杂志文章,评审
doi:10.1016/0923-2508(90)90114-6
更新日期:1990-09-01 00:00:00
abstract::This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80078-8
更新日期:1998-03-01 00:00:00
abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.08.001
更新日期:2006-04-01 00:00:00
abstract::Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)84020-4
更新日期:1996-10-01 00:00:00
abstract::Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.04.003
更新日期:2009-06-01 00:00:00
abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/s0923-2508(01)01279-7
更新日期:2002-01-01 00:00:00
abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90193-9
更新日期:1989-11-01 00:00:00
abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(92)90061-r
更新日期:1992-09-01 00:00:00
abstract::The association between bacteria and leaves in Ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. This intimate interaction suggests that both partners have co-diversified to...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2011.04.003
更新日期:2011-06-01 00:00:00
abstract::Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/j.resmic.2005.01.014
更新日期:2005-06-01 00:00:00
abstract::DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus. In t...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2017.12.006
更新日期:2018-04-01 00:00:00
abstract::Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2008.05.004
更新日期:2008-07-01 00:00:00
abstract::Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2009.03.003
更新日期:2009-04-01 00:00:00
abstract::Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.03.001
更新日期:2014-04-01 00:00:00
abstract::The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.07.006
更新日期:2006-04-01 00:00:00
abstract::Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were func...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.04.011
更新日期:2018-01-01 00:00:00