Deciphering the molecular basis of multidrug recognition: crystal structures of the Staphylococcus aureus multidrug binding transcription regulator QacR.

Abstract:

:Multidrug transporters and their transcriptional regulators are key components of bacterial multidrug resistance (MDR). How these multidrug binding proteins can recognize such chemically disparate compounds represents a fascinating question from a structural standpoint and an important question in future drug development efforts. The Staphylococcus aureus multidrug binding regulator, QacR, is soluble and recognizes an especially wide range of structurally dissimilar compounds, properties making it an ideal model system for deciphering the molecular basis of multidrug recognition. Recent structures of QacR have afforded the first view of any MDR protein bound to multiple drugs, revealing key structural features of multidrug recognition, including a multisite binding pocket.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Schumacher MA,Brennan RG

doi

10.1016/S0923-2508(02)00013-X

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

69-77

issue

2

eissn

0923-2508

issn

1769-7123

pii

S0923-2508(02)00013-X

journal_volume

154

pub_type

杂志文章,评审
  • Comparative performance of different PCR assays for the identification of Campylobacter jejuni and Campylobacter coli.

    abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2007.11.020

    authors: Debruyne L,Samyn E,De Brandt E,Vandenberg O,Heyndrickx M,Vandamme P

    更新日期:2008-03-01 00:00:00

  • Bacillus subtilis isolated from the human gastrointestinal tract.

    abstract::As part of an ongoing study to determine the true habitat of Bacillus species, we report here the isolation and characterisation of Bacillus subtilis from the human gastrointestinal tract (GIT). Strains were obtained from ileum biopsies as well as from faecal samples and their biotypes defined. 16S rRNA analysis revea...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2008.11.002

    authors: Hong HA,Khaneja R,Tam NM,Cazzato A,Tan S,Urdaci M,Brisson A,Gasbarrini A,Barnes I,Cutting SM

    更新日期:2009-03-01 00:00:00

  • Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level.

    abstract::Bacillus species as a group offer several advantages over other bacteria for protection against root pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. The objectives of this work were to determine the ability of strains of Bacillus to inhibit Fusari...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.03.001

    authors: Cavaglieri L,Orlando J,Rodríguez MI,Chulze S,Etcheverry M

    更新日期:2005-06-01 00:00:00

  • Differentiation of Candida guilliermondii varieties by lectin-like substances from marine algae.

    abstract::Lectin-like substances of 54 marine algae, corresponding to 30 red algae and 24 brown algae, were studied to estimate the agglutination activity against Candida guilliermondii. Extracts of Dictyopteris membranacea, Bifurcaria bifurcata, Halidrys siliquosa, Ascophyllum nodosum, Fucus serratus and F. spiralis algae show...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(89)90013-2

    authors: Fabregas J,Muñoz A,Llovo J,Villa TG

    更新日期:1989-07-01 00:00:00

  • The LPS localization might explain the lack of protection of LPS-specific antibodies in abortion-causing Chlamydia psittaci infections.

    abstract::Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infect...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(94)90078-7

    authors: Salinas J,Sanchez J,Buendia AJ,Souriau A,Rodolakis A,Bernabé A,Cuello F

    更新日期:1994-10-01 00:00:00

  • Cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Lactobacillus plantarum LA 318 recognizes human A and B blood group antigens.

    abstract::Lactobacillus plantarum LA 318 is a potential probiotic strain isolated from normal human intestinal tissue that shows high adhesion to human colonic mucin mediated by the bacterial cell surface glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We report the adhesion mechanism of the lactobacilli is in part due to GAP...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2008.07.005

    authors: Kinoshita H,Wakahara N,Watanabe M,Kawasaki T,Matsuo H,Kawai Y,Kitazawa H,Ohnuma S,Miura K,Horii A,Saito T

    更新日期:2008-11-01 00:00:00

  • Exopolysaccharides produced by the recently described halophilic bacteria Halomonas ventosae and Halomonas anticariensis.

    abstract::We studied exopolysaccharides (EPSs) produced by Halomonas ventosae and Halomonas anticariensis, two novel species of halophilic bacteria. Under optimum environmental and nutritional conditions, H. ventosae strains Al12(T) and Al16 excreted 28.35 mg and 28.95 mg of EPS per 100 ml of culture medium (34.55 and 38.6 mg o...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2006.06.004

    authors: Mata JA,Béjar V,Llamas I,Arias S,Bressollier P,Tallon R,Urdaci MC,Quesada E

    更新日期:2006-11-01 00:00:00

  • Efficient respiration on TMAO requires TorD and TorE auxiliary proteins in Shewanella oneidensis.

    abstract::Respiration on trimethylamine oxide (TMAO) allows bacterial survival under anoxia. In Shewanella oneidensis, Tor is the system involved in TMAO respiration and it is encoded by the torECAD operon. The torA and torC genes encode TorA terminal reductase and the TorC c-type cytochrome, respectively. Sequence analysis sug...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2016.05.004

    authors: Lemaire ON,Honoré FA,Jourlin-Castelli C,Méjean V,Fons M,Iobbi-Nivol C

    更新日期:2016-10-01 00:00:00

  • Comparison of molecular epidemiological tools for Branhamella catarrhalis typing.

    abstract::Twenty-one Branhamella catarrhalis strains selected for their diversity by esterase electrophoretic polymorphism, and belonging to 20 distinct zymotypes, were studied by restriction fragment length polymorphism (RFLP) of total DNA after ethidium bromide staining and of ribosomal DNA regions (ribotyping). The former an...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(91)90191-c

    authors: Denamur E,Picard-Pasquier N,Mura C,Picard B,Orfila J,Krishnamoorthy R

    更新日期:1991-06-01 00:00:00

  • Cloning and characterization of PRB1, a Candida albicans gene encoding a putative novel endoprotease B and factors affecting its expression.

    abstract::Several cDNA fragments corresponding to transcripts differentially expressed under conditions that favor mycelial growth of Candida albicans were identified by the "differential display" technique. One of these was cloned and used as a probe to rescue the full gene from a genomic library of the fungus. The sequence id...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(02)01373-6

    authors: Orozco I,Ortiz L,Elorza MV,Ruiz-Herrera J,Sentandreu R

    更新日期:2002-11-01 00:00:00

  • Colicin N interaction with sensitive Escherichia coli cells: in situ and kinetic approaches.

    abstract::The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sen...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(99)80012-6

    authors: el Kouhen R,Bernadac A,Pagès JM

    更新日期:1998-10-01 00:00:00

  • Probiotic activities of Lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties.

    abstract::The interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. In this study, the probiotic activities of a human isolate of Lactobacillus casei subsp. rhamnosu...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(01)01188-3

    authors: Forestier C,De Champs C,Vatoux C,Joly B

    更新日期:2001-03-01 00:00:00

  • Cross-correlating analyses of mineral-associated microorganisms in an unsaturated packed bed flow-through column test; cell number, activity and EPS.

    abstract::In heap bioleaching and waste-rock dumps, complex microbial communities exist in the flowing and interstitial liquid phases and mineral surface-associated biofilms, often embedded in extracellular polymeric substances (EPS). Microbial activity in the interstitial phase and mineral ore surface facilitates mineral degra...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2020.09.002

    authors: Makaula DX,Huddy RJ,Fagan-Endres MA,Harrison STL

    更新日期:2020-01-01 00:00:00

  • Evaluation of the use of 5-mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl- alpha-arabinofuranosyl-(1-->1')-glycerol in serodiagnosis of Mycobacterium avium-intracellulare complex infection.

    abstract::5-Mycoloyl-beta-arabinofuranosyl-(1-->2)-5-mycoloyl-alpha-ar abinofuranosyl-(1-->1')-glycerol, an antigenic glycolipid from the Mycobacterium avium-intracellulare complex (MAC) was examined for its applicability to the serodiagnosis of MAC infection by ELISA. Serum IgM antibody titres against this glycolipid in health...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(93)90048-7

    authors: Honda I,Kawajiri K,Watanabe M,Toida I,Kawamata K,Minnikin DE

    更新日期:1993-03-01 00:00:00

  • Clinical and field trials with attenuated Salmonella typhi as live oral vaccines and as "carrier" vaccines.

    abstract::In recent years there has been a resurgence of research to develop new and improved attenuated strains of Salmonella typhi to function as live oral vaccines against typhoid fever and to serve as "carrier" vaccines to express foreign antigens of other pathogens and deliver them to the immune system. Strain Ty21a has se...

    journal_title:Research in microbiology

    pub_type: 临床试验,杂志文章,评审

    doi:10.1016/0923-2508(90)90114-6

    authors: Levine MM,Hone D,Tacket C,Ferreccio C,Cryz S

    更新日期:1990-09-01 00:00:00

  • Cloning and sequence determination of a phi AAU2 gene whose product aborts the phage lytic cycle.

    abstract::This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower eff...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/s0923-2508(98)80078-8

    authors: Le Marrec C,Moreau S,Leret V,Schäffer A,Blanco C,Trautwetter A

    更新日期:1998-03-01 00:00:00

  • Expression, characterization and immunolocalization of translation termination factor eRF3 in the ciliate Euplotes octocarinatus.

    abstract::Class II polypeptide release factor eRF3 stimulates translation termination in a GTP-dependent manner. Recent data show that eRF3 is also involved in cell cycle regulation apart from its function in translation. Here we show that recombinant Eo-eRF3 from the ciliate Euplotes octocarinatus expressed in Escherichia coli...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.08.001

    authors: Chai BF,Wang W,Liang AH

    更新日期:2006-04-01 00:00:00

  • Identification of Listeria species by a semi-nested polymerase chain reaction.

    abstract::Three primers derived from the lap gene were used to distinguish Listeria monocytogenes from L.innocua and other Listeria species. L. monocytogenes and L. innocua yielded a PCR product of 600 and 300 bp, respectively, whereas a typical pattern of three amplimers was observed with L. ivanovii, L. seeligeri and L. welsh...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(96)84020-4

    authors: Manzano M,Cocolin L,Ferroni P,Gasparini V,Narduzzi D,Cantoni C,Comi G

    更新日期:1996-10-01 00:00:00

  • A combination of a SEM technique and X-ray microanalysis for studying the spore germination process of Clostridium tyrobutyricum.

    abstract::Clostridium tyrobutyricum is an anaerobic bacterium responsible for late blowing defects during cheese ripening and it is of scientific interest for biological hydrogen production. A scanning electron microscopy (SEM) coating technique and X-ray microanalysis were developed to analyze the architecture and chemical com...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2009.04.003

    authors: Bassi D,Cappa F,Cocconcelli PS

    更新日期:2009-06-01 00:00:00

  • Life and death of dried prokaryotes.

    abstract::The removal of water through air drying damages membranes, proteins and nucleic acids and is lethal to the majority of organisms. Nevertheless, some vegetative cells of bacteria and cyanobacteria survive extreme desiccation. Understanding the mechanisms of their desiccation tolerance is an important issue in cell biol...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/s0923-2508(01)01279-7

    authors: Billi D,Potts M

    更新日期:2002-01-01 00:00:00

  • rRNA gene restriction patterns of Legionella species: a molecular identification system.

    abstract::A total of 28 species of Legionella could be differentiated by rRNA gene restriction patterns generated after cleavage of total DNA with either EcoRV or HindIII restriction endonucleases, and hybridization of fragments with 32P-labelled Escherichia coli 16 + 23S rRNA. Different species gave different fragment patterns...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(89)90193-9

    authors: Grimont F,Lefèvre M,Ageron E,Grimont PA

    更新日期:1989-11-01 00:00:00

  • Unusual structure of plasmid DNA formed in transformants of Escherichia coli.

    abstract::An unusual structural component of plasmid pUC18 was observed in transformants of Escherichia coli strains. This molecule migrated faster than superhelical topoisomers in agarose gel electrophoresis and was difficult to stain with ethidium bromide. This unusual topology appeared independently of gyrase mutation, and t...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/0923-2508(92)90061-r

    authors: Kato M,Furuno A

    更新日期:1992-09-01 00:00:00

  • Bacterial leaf symbiosis in Ardisia (Myrsinoideae, Primulaceae): molecular evidence for host specificity.

    abstract::The association between bacteria and leaves in Ardisia has been described as a cyclic and obligate symbiosis in which bacteria are maintained throughout all stages of the plant's life cycle to guarantee normal growth and survival of the host. This intimate interaction suggests that both partners have co-diversified to...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2011.04.003

    authors: Lemaire B,Smets E,Dessein S

    更新日期:2011-06-01 00:00:00

  • Was photosynthetic RuBisCO recruited by acquisitive evolution from RuBisCO-like proteins involved in sulfur metabolism?

    abstract::Genome analyses have revealed that the genomes of non-photosynthetic bacteria including Bacillus subtilis code for proteins similar to the large subunit of RuBisCO (called RuBisCO-like protein (RLP)). This raises a fundamental question as to their functional relationship to photosynthetic RuBisCO. Recently, we identif...

    journal_title:Research in microbiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resmic.2005.01.014

    authors: Ashida H,Danchin A,Yokota A

    更新日期:2005-06-01 00:00:00

  • Regulator DegU is required for multicellular behavior in Lysinibacillus sphaericus.

    abstract::DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus. In t...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2017.12.006

    authors: Hu Y,Cai Q,Tian S,Ge Y,Yuan Z,Hu X

    更新日期:2018-04-01 00:00:00

  • Temperature dependence of mycosubtilin homologue production in Bacillus subtilis ATCC6633.

    abstract::Bacillus subtilis ATCC6633 produces mycosubtilin, a non-ribosomally synthesized lipopeptide of the iturin family which presents antagonistic activities toward various phytopathogens. Different homologues with fatty acid moiety varying from C15 to C17 are usually co-produced, with their biological activities increasing...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2008.05.004

    authors: Fickers P,Leclère V,Guez JS,Béchet M,Coucheney F,Joris B,Jacques P

    更新日期:2008-07-01 00:00:00

  • Subinhibitory fluoroquinolone exposure selects for reduced beta-lactam susceptibility in methicillin-resistant Staphylococcus aureus and alterations in the SOS-mediated response.

    abstract::Growth of fluoroquinolone (FQ)-susceptible methicillin-resistant Staphylococcus aureus (MRSA) in the presence of sub-MIC FQ has been shown to enhance methicillin resistance in traditional nosocomial MRSA isolates. We aimed to confirm this phenomenon in nine community-associated MRSA (CA-MRSA) clinical isolates, and to...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2009.03.003

    authors: Tattevin P,Basuino L,Chambers HF

    更新日期:2009-04-01 00:00:00

  • Novel role of the Candida albicans ferric reductase gene CFL1 in iron acquisition, oxidative stress tolerance, morphogenesis and virulence.

    abstract::Ferric reductase catalyzes the reduction of ferric iron into ferrous iron and plays an essential role in high-affinity iron acquisition. In this study, we found that the cfl1Δ/Δ (orf19.1263) mutant was not defective in iron acquisition. However, deletion of CFL1 increased cellular iron accumulation by elevating surfac...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2014.03.001

    authors: Xu N,Qian K,Dong Y,Chen Y,Yu Q,Zhang B,Xing L,Li M

    更新日期:2014-04-01 00:00:00

  • Real-time PCR for characterizing the stress response of Oenococcus oeni in a wine-like medium.

    abstract::The tolerance of the lactic acid bacterium Oenococcus oeni to hostile wine conditions is essential for the success of malolactic fermentation (MLF). In this study, reverse transcription quantitative PCR (RT-qPCR) was used to quantify the transcript level of 13 genes that could play a role in adaptation of O. oeni in w...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2005.07.006

    authors: Beltramo C,Desroche N,Tourdot-Maréchal R,Grandvalet C,Guzzo J

    更新日期:2006-04-01 00:00:00

  • Expression and deletion analyses of cspE encoding cold-shock protein E in Acinetobacter oleivorans DR1.

    abstract::Six genes encoding cold-shock-like proteins, including cspE, are contained within the genome of Acinetobacter oleivorans DR1. All six genes are similar in size as well as amino acid identity, but appear to be differentially regulated under stressful conditions. Four of these genes (cspA, cspB, cspC and cspE) were func...

    journal_title:Research in microbiology

    pub_type: 杂志文章

    doi:10.1016/j.resmic.2018.04.011

    authors: Kim J,Ha S,Park W

    更新日期:2018-01-01 00:00:00