Abstract:
:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infected with the strain, were performed. Immunogold labelling was observed on the surface of reticular bodies (RB), but not on elementary bodies (EB). Immunolabelling was observed in ultrathin sections on both sides of the external chlamydial membrane, mainly on the inner side of EB and on the outer side of RB. Immunogold density was higher in EB than in RB; however, the absolute number of gold particles was higher in RB than EB, suggesting a loss of immunolabelling during the transformation of RB into EB. Specific labelling of LPS was also found in electrodense and adielectronic vacuoles near the surface of the cytoplasmic membrane of infected McCoy cells. These results suggest that the lack of protection against some chlamydial strains, despite the presence of anti-LPS specific antibodies, is due to the localization of LPS on the inner side of the external membrane of EB.
journal_name
Res Microbioljournal_title
Research in microbiologyauthors
Salinas J,Sanchez J,Buendia AJ,Souriau A,Rodolakis A,Bernabé A,Cuello Fdoi
10.1016/0923-2508(94)90078-7subject
Has Abstractpub_date
1994-10-01 00:00:00pages
611-20issue
8eissn
0923-2508issn
1769-7123pii
0923-2508(94)90078-7journal_volume
145pub_type
杂志文章abstract::Biological nitrogen fixation (BNF) is a tightly regulated process that is carried out by diazotrophic microorganisms. The regulatory mechanisms of BNF-related genes are well characterized in Gram-negative models, but they are poorly understood in Gram-positive bacteria. Paenibacillus riograndensis SBR5(T) is a Gram-po...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2014.06.002
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80281-6
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doi:10.1016/s0923-2508(01)01263-3
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abstract::KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity, which is specifically induced by ethanol and insensitive to glucose repression. In this work, we report the molecular analysis of UAS(E), an element of the KlADH4 promoter which is essential for the induction of KlADH4 in...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(00)00131-5
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abstract::In contrast to bacteria, mitochondria contain only a few ATP binding cassette (ABC) transporters in their inner membrane. The known mitochondrial ABC proteins fall into two major classes that, in the yeast Saccharomyces cerevisiae, are represented by the half-transporter Atm1p and the two closely homologous proteins M...
journal_title:Research in microbiology
pub_type: 杂志文章,评审
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abstract::This paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of L. monocyto...
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2016.05.009
更新日期:2016-10-01 00:00:00
abstract::Random amplified polymorphic DNA (RAPD) analysis was applied to genomic DNA from nineteen yeast strains belonging to the genera Saccharomyces and Zygosaccharomyces. Results obtained with five primers indicated that this technique is a powerful tool for yeast differentiation and identification. The data were consistent...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(96)80565-1
更新日期:1995-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 传,历史文章,杂志文章,评审
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.09.013
更新日期:2010-12-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章,评审
doi:10.1016/0923-2508(92)90117-7
更新日期:1992-07-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80323-9
更新日期:1998-06-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章,评审
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(93)90004-l
更新日期:1993-09-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(99)00110-2
更新日期:1999-09-01 00:00:00
abstract::During an epidemiologic survey, an unidentified strain of Legionella was isolated from water of a thermal spa in France. The strain (Lyon 8420412) had the cultural and biochemical characteristics typical of the genus Legionella. In direct immunofluorescence tests, the strain reacted weakly with fluorescein-conjugated ...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(89)90086-7
更新日期:1989-10-01 00:00:00
abstract::Here we characterize the first step in methionine biosynthesis in Agrobacterium tumefaciens, an α-proteobacterium. We explored the metA gene and its products and found several unique properties. Although the gene was annotated as a homoserine transsuccinylase, based upon sequence similarity to characterized homologs i...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2012.10.005
更新日期:2013-01-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2005.05.012
更新日期:2005-11-01 00:00:00
abstract::Some of the present problems in ribotyping are associated with a lack of uniform reactivity of probes when bacterial DNAs are of phylogenetically diverse origins. To overcome these problems, a set of five oligonucleotides (referred to as OligoMix5) was selected to react with conserved sequences located near both extre...
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pub_type: 杂志文章
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journal_title:Research in microbiology
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90047-e
更新日期:1991-02-01 00:00:00
abstract::The sensitivity and specificity of 7 PCR assays described for the identification of Campylobacter jejuni and Campylobacter coli were examined using alkaline cell lysates from a collection of 100 well characterized reference strains of C. jejuni, C. coli, Campylobacter lari and related Campylobacter, Helicobacter and A...
journal_title:Research in microbiology
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(94)90042-6
更新日期:1994-11-01 00:00:00
abstract::Rhodococcus strains are important biocatalysts used for biotechnological production of acrylamide catalysed by a nitrile hydratase (NHase) containing cobalt. This metalloenzyme is present at high intracellular concentrations representing up to 50% of the soluble proteins in Rhodococcus rhodochrous M8 strain. Cobalt io...
journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2018.03.006
更新日期:2018-01-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/0923-2508(91)90191-c
更新日期:1991-06-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
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更新日期:2014-11-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/j.resmic.2010.07.006
更新日期:2010-11-01 00:00:00
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journal_title:Research in microbiology
pub_type: 杂志文章
doi:10.1016/s0923-2508(98)80304-5
更新日期:1998-04-01 00:00:00
abstract::Helicobacter spp. have been the focus of considerable research because of the role of this genus in gastrointestinal diseases. We infected NIH germ-free mice with Helicobacter trogontum, a recently described intestinal bacterium of rats, in order to study the distribution of this bacterium in the gastrointestinal trac...
journal_title:Research in microbiology
pub_type: 杂志文章
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