The LPS localization might explain the lack of protection of LPS-specific antibodies in abortion-causing Chlamydia psittaci infections.

Abstract:

:Four monoclonal antibodies against chlamydial lipopolysaccharide (LPS) were used to study their localization and distribution in the Chlamydia psittaci AB7 abortion-causing strain by immunoelectron microscopy. A non-embedding technique on whole chlamydiae, together with a post-embedding technique on McCoy cells infected with the strain, were performed. Immunogold labelling was observed on the surface of reticular bodies (RB), but not on elementary bodies (EB). Immunolabelling was observed in ultrathin sections on both sides of the external chlamydial membrane, mainly on the inner side of EB and on the outer side of RB. Immunogold density was higher in EB than in RB; however, the absolute number of gold particles was higher in RB than EB, suggesting a loss of immunolabelling during the transformation of RB into EB. Specific labelling of LPS was also found in electrodense and adielectronic vacuoles near the surface of the cytoplasmic membrane of infected McCoy cells. These results suggest that the lack of protection against some chlamydial strains, despite the presence of anti-LPS specific antibodies, is due to the localization of LPS on the inner side of the external membrane of EB.

journal_name

Res Microbiol

journal_title

Research in microbiology

authors

Salinas J,Sanchez J,Buendia AJ,Souriau A,Rodolakis A,Bernabé A,Cuello F

doi

10.1016/0923-2508(94)90078-7

subject

Has Abstract

pub_date

1994-10-01 00:00:00

pages

611-20

issue

8

eissn

0923-2508

issn

1769-7123

pii

0923-2508(94)90078-7

journal_volume

145

pub_type

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